The analysis for differentiation and function of human cytotoxic T lymphocytes under exposure to asbestos

• Project/Area Number: 20890270
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Hygiene
• Research Institution: Kawasaki Medical School
• Principal Investigator: KUMAGAI Naoko Kawasaki Medical School, 医学部, 助教 (00509276)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 細胞傷害性T細胞 / 石綿 / 悪性中皮腫 / IFN-γ / Granzyme B / CTL / CD8

Research Abstract

The effect of asbestos-exposure on CTL was investigated. Exposure to CB suppressed an increase in CD8^+ cell-number, cytotoxicity, increases in intracellular granzyme B and IFN-γ levels, and differentiation from naive cells to CTL. CB-exposure suppressed the productions of IL-10, IFN-γ, and TNF-α, but not IL-2. These results deny the possible contribution of IL-10, representative suppressive cytokine, to the suppressed induction of CTL, and indicate the potential of asbestos to inhibit the induction of CTL with decreases in IFN-γ and TNF-α, representative promoting cytokines. These suggest that inhaled asbestos might promote the generation of tumor disease by suppression of tumor-immunity.

Research Products

• [Journal Article] Impairment in cytotoxicity and expression of NK-cell activating receptors on human NK cells following exposure to asbestos fibers. (2009)
  • Author(s): Nishimura Y, Miura Y, Maeda M, Kumagai N, Murakami S, Hayashi H, Fukuoka K, Nakano T, Otsuki T
  • Journal Title: Int J Immunopathol Pharmacol Vol.22,No.3 Pages: 579-590
  • Peer Reviewed
• [Journal Article] Decrease in phosphorylation of ERK following decreased expression of NK-cell activating receptors in human NK cell line exposed to asbestos. (2009)
  • Author(s): Nishimura Y, Maeda M, Kumagai N, Hayashi H, Miura Y, Otsuki T
  • Journal Title: Int J Immunopathol Pharmacol Vol.22,No.4 Pages: 879-888
  • Peer Reviewed
• [Journal Article] Cytokine alteration and speculated immunological pathophysiology in silicosis and asbestos-related diseases. (2009)
  • Author(s): Murakami S, Nishimura Y, Maeda M, Kumagai N, Hayashi H, Chen Y, Kusaka K, Kishimoto T, Otsuki t
  • Journal Title: Environ Health Prev Med Vol.14,No.4 Pages: 216-222
  • NAID: 10026958932
  • Peer Reviewed
• [Journal Article] Is soluble CD40 ligand an indicator of immunopathological disturbance in silicosis patients? (2009)
  • Author(s): Hayashi H, Nishimura Y, Maeda M, Murakami S, Kumagai N, ChenY, Kusaka M, Uragami K, Fujimoto W, OtsukiT
  • Journal Title: Kawasaki Med J Vol.35,No.2 Pages: 129-138
  • NAID: 110007150517
  • Peer Reviewed
• [Journal Article] Soluble interleukin-2 receptor as an indicator of immunological disturbance found in silicosis patients. (2009)
  • Author(s): Hayashi, H, Maeda, M, Murakami, S, Kumagai, N, Chen, Y, Hatayama, T, Katoh, M, Miyahara, N, Yamamoto, S, Yoshida, Y, Nishimura, Y, Kusaka, M, Fujimoto, W, Otsuki, T
  • Journal Title: Int J Immunopathol Pharmacol Vol.22,No.1 Pages: 53-62
  • Peer Reviewed
• [Journal Article] Impairment in cytotoxicity and expression of NK-cell activating receptors on human NK cells following exposure to asbestos fibers (2009)
  • Author(s): Yasumitsu Nishimura
  • Journal Title: International Journal of Immunopatholoy and Pharmacology 22 Pages: 579-590
  • Peer Reviewed
• [Journal Article] Decrease in phosphorylation of ERK following decreased expression of NK-cell activating receptors in human NK cell line exposed to asbestos (2009)
  • Author(s): Yasumitsu Nishimura
  • Journal Title: International Journal of Immunopatholoy and Pharmacology 22 Pages: 879-888
  • Peer Reviewed
• [Journal Article] Is soluble CD40 ligand an indicator of immunopathological disturbance in silicosis patients? (2009)
  • Author(s): Hiroaki Hayashi
  • Journal Title: Kawasaki Medical Journal 35 Pages: 129-138
  • Peer Reviewed
• [Journal Article] Cytokine alteration and speculated immunological pathophysiology in silicosis and asbestos-related diseases (2009)
  • Author(s): Shuko Murakami
  • Journal Title: Environmental Health and Preventive Medicine 14 Pages: 216-222
  • Peer Reviewed
• [Journal Article] Soluble interleukin-2 receptor as an indicator of immunological disturbance found in silicosis patients. (2009)
  • Author(s): Hiroaki Hayashi
  • Journal Title: International Journal of Immunopatholoy and Pharmacology 22 Pages: 53-62
  • Peer Reviewed
• [Journal Article] 珪肺症例CD4+25+分画における活性化T細胞の混入と制御性T細胞早期細胞死の可能性 (2009)
  • Author(s): 林宏明
  • Journal Title: 日本職業・環境アレルギー学会雑誌 15 Pages: 43-50
  • Peer Reviewed
• [Journal Article] 新しい環境中物質, 繊維・粒子状物質による生体影響へのアプローチ-シンポジウム「日本臨床環境医学の近未来」によせて- (2009)
  • Author(s): 熊谷直子
  • Journal Title: 臨床環境医学 17 Pages: 83-94
  • Peer Reviewed
• [Journal Article] 新しい環境中物質, 繊維・粒子状物質による生体影響へのアプローチ-シンポジウム「日本臨床環境医学の近未来」によせて. (2008)
  • Author(s): 熊谷直子, 西村泰光, 前田恵, 村上周子, 林宏明, 陳瑩, 平野靖史郎, 大槻剛巳
  • Journal Title: 臨床環境医学 Vol.17 Pages: 83-94
  • Peer Reviewed
• [Journal Article] Immunological changes in mesothelioma patients and their experimental detection. (2008)
  • Author(s): Maeda M, Miura Y, Nishimura Y, Murakami S, Hayashi H, Kumagai N, Hatayama T, Katoh M, Miyahara N, Yamamoto S, Fukuoka K, KishimotoT, Nakano T
  • Journal Title: Clin Med: Circ Resp Pulm Med Vol.2 Pages: 11-17
  • Peer Reviewed
• [Journal Article] アスベスト関連疾患の発症機構ー悪性中脾腫を中心としてー. (2008)
  • Author(s): 西村泰光, 前田恵, 村上周子, 熊谷直子, 林宏明, 大槻剛巳
  • Journal Title: Surgery Frontier Vol.15,No.2 Pages: 8-13
• [Journal Article] 珪肺症例CD4+CD25+分画における活性化T細胞の混入と制御性T細胞の早期細胞死の可能性. (2008)
  • Author(s): 林宏明, 三浦由恵, 村上周子, 熊谷直子, 西村泰光, 草加勝康, 大槻剛巳
  • Journal Title: 日本職業・環境アレルギー誌 Vol.15,No.2 Pages: 43-50
  • Peer Reviewed
• [Journal Article] Lifestyle and T-helper 1 and 2 related cytokines in healthy volunteers. (2008)
  • Author(s): Miura Y, Ishibashi T, Tatsukawa T, Maeda M, Murakami S, Nisimura Y, Kumagai N, Hayashi H, Chen Y, Hyodo Y, Hatta E, Kojima S, Fujii M, Morimoto K, Otsuki T
  • Journal Title: KAWASAKI MED J Vol.34,No.3 Pages: 171-177
  • NAID: 110006936082
  • Peer Reviewed
• [Journal Article] Immunological changes in mesothelioma patients and their experimental detection. (2008)
  • Author(s): Megumi Maeda
  • Journal Title: Clinical Medicine : Circulatory, Respiratory and Pulmonary 2 Pages: 11-17
  • Peer Reviewed
• [Journal Article] Lifestyle and T-helper 1 and 2 related cytokines in healthy volunteers. (2008)
  • Author(s): Yoshie Miura
  • Journal Title: Kawasaki Medical Journal 34 Pages: 171-177
  • Peer Reviewed
• [Journal Article] アスベスト関連疾患の発症機構-悪性中脾腫を中心として- (2008)
  • Author(s): 西村泰光
  • Journal Title: Surgery Frontier 15 Pages: 8-13
• [Presentation] Suppressive effects of asbestos-exposure on the differentiation into human cytotoxic T lymphocytes. (2010)
  • Author(s): Kumagai N, Nishimura Y, Maeda M, Hayashi H, Otsuki T
  • Organizer: 49th Annual Meeting of the Society of Toxicology
  • Place of Presentation: Salt Lake City, Utah
  • Year and Date: 2010-03-10
• [Presentation] Suppressive effects of asbestos-exposure on the differentiation into human cytotoxic T lymphocytes (2010)
  • Author(s): 熊谷直子
  • Organizer: 49^<TH> Annual Meeting of The Society of Toxicology
  • Place of Presentation: Salt Lake City, Utah
  • Year and Date: 2010-03-10
• [Presentation] Analysis for effect of asbestos-exposure on production of cytokines related with differentiation into human CTL. (2009)
  • Author(s): Kumagai N, Nishimura Y, maeda M, Hayashi H, 0tsuki T
  • Organizer: 第39回日本免疫学会学術大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-04
• [Presentation] Analysis for effect of asbestos-exposure on production of cytokines related with differentiation into human CTL (2009)
  • Author(s): 熊谷直子
  • Organizer: 第39回日本免疫学会学術大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-04
• [Presentation] 石綿曝露によるCTL分化抑制に関わるサイトカイン産生について. (2009)
  • Author(s): 熊谷直子, 西村泰光, 前田恵, 林宏明, 大槻剛巳
  • Organizer: 第16回日本免疫毒性学会学術大会
  • Place of Presentation: 旭川市民文化会館
  • Year and Date: 2009-08-28
• [Presentation] 石綿曝露によるCTL分化抑制に関わるサイトカイン産生について (2009)
  • Author(s): 熊谷直子
  • Organizer: 第16回日本免疫毒性学会学術大会
  • Place of Presentation: 旭川市民文化会館
  • Year and Date: 2009-08-28
• [Presentation] 石綿曝露によるヒト細胞傷害性T細胞分化の抑制. (2009)
  • Author(s): 熊谷直子, 西村泰光, 前田恵, 村上周子, 林宏明, 陳瑩, 大槻剛巳
  • Organizer: 第79回日本衛生学会学術総会
  • Place of Presentation: 北里大学白金キャンパス
  • Year and Date: 2009-03-30
• [Presentation] 石綿曝露によるヒト細胞傷害性T細胞分化の抑制 (2009)
  • Author(s): 熊谷直子
  • Organizer: 第79回日本衛生学会学術総会
  • Place of Presentation: 東京, 北里大学白金キャンパス
  • Year and Date: 2009-03-30
• [Presentation] The analysis for function of human cytotoxic T lymphocytes induced under exposure to asbestos. (2008)
  • Author(s): Kumagai N, Nishimura Y, Maeda M, Murakami S, Hayashi H, Chen Y, Otsuki Takemi
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-12-01
• [Presentation] The analysis for function of human cytotoxic T lymphocytes induced under exposure to asbestos (2008)
  • Author(s): Naoko Kumagai
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都, 国立京都国際会館
  • Year and Date: 2008-12-01
• [Presentation] ヒト細胞傷害性T細胞の機能に及ぼす石綿曝露の影響. (2008)
  • Author(s): 熊谷直子, 西村泰光, 前田恵, 村上周子, 林宏明, Chen Ying, 大槻剛巳
  • Organizer: 第15回日本免疫毒性学会学術大会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2008-09-12
• [Presentation] ヒト細胞傷害性T細胞の機能に及ぼす石綿曝露の影響 (2008)
  • Author(s): 熊谷直子
  • Organizer: 第15回日本免疫毒性学会学術大会
  • Place of Presentation: 東京, タワーホール船堀
  • Year and Date: 2008-09-12
• [Book] Immunological effects of Asbestos. In Asbestos: Risks, Environment and Impact., Eds: Antonio Soto and Gael Salazar (2009)
  • Author(s): Otsuki T, Maeda M, Miura Y, Hayashi H, Murakami S, Kumagai N, Nishimura Y
  • Publisher: Nova Science Publishers
• [Book] Immunological effects of Asbestos. In Asbestos : Risks, Environment and Impact (2009)
  • Author(s): Takemi Otsuki
  • Total Pages: 9
  • Publisher: Nova Science Publishers, Inc