| Project/Area Number |
20890292
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
TANEGASHIMA Kosuke Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 主任研究員 (20507678)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
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| Keywords | 先天異常学 / 神経管閉鎖障害 / 発生・分化 / 脳・神経 / ノックアウトマウス / WGEF / シグナル伝達 / ES細胞 / 相同組み替え |
|---|
| Research Abstract |
Abnormal morphogenesis of the neural tube leads to neural tube defects including spina bifida and anencephaly. We found that a novel guanine nucleotide exchanging factor, WGEF mediates Wnt-PCP pathway, and regulates morphogenic movements of neural tube in the amphibian embryo. In this study, I attempted to generate a conditional knockout mouse of WGEF gene as a mammalian model of neural tube defects. So far, a conditional knockout allele on the WGEF locus was introduced by homologous recombination in the ES cells. Using this ES cells, chimera mice carrying WGEF conditional knockout cells have been generated.
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