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Detection of T790M Gefitinib Resistance Mutation in EGFR using the BEAMing method

Research Project

Project/Area Number 20890299
Research Category

Grant-in-Aid for Young Scientists (Start-up)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionResearch Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses

Principal Investigator

TANIGUCHI Kazuya  Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses, 研究所, 研究員 (70463289)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
Keywords非小細胞肺癌 / ゲフィチニブ / EGFR / 抗癌剤耐性 / 遺伝子変異 / 遺伝子診断 / 分子標的薬
Research Abstract

Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs), such as gefitinib or erlotinib, are effective therapies for non-small cell lung cancer (NSCLC) patients with primary EGFR mutations. However, most patients who initially respond to treatment with these drugs will develop resistance to EGFR TKIs. A secondary EGFR T790M mutation is reported to correlate with acquired resistance. We examined the T790M mutation in primary tumors with BEAMing (beads, emulsion, amplification, magnetic), which is the most sensitive technique to detect minor mutated alleles. Currently, we can detect point mutation at the level of 1/10,000 using an improved version of the BEAMing. We detected T790M mutant alleles in NSCLCs in 20 out of 270 NSCLCs with primary EGFR mutations. The detection of small fraction of T790M mutant alleles may be useful for predicting resistance of NSCLCs to TKIs.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (13 results)

All 2009 2008

All Journal Article (1 results) Presentation (12 results)

  • [Journal Article] Intratumor heterogeneity of epidermal growth factor receptor mutations in lung cancer and its correlation to the response to gefitinib2008

    • Author(s)
      Taniguchi K, Okami J, Kodama K, Higashiyama M, Kato K
    • Journal Title

      Cancer Sci 99(5)

      Pages: 929-35

    • NAID

      10024005970

    • Related Report
      2009 Final Research Report
  • [Presentation] Detection of EGFR Gene T790M mutation in Non-Small Cell Lung Cancer using an improved version of the BEAMing technology2009

    • Author(s)
      Kimiyoshi Nishitani, Kazuya Taniguchi, Jiro Okami, Ken Kodama, Masahiko Higashiyama, kikuya kato
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-01
    • Related Report
      2009 Final Research Report
  • [Presentation] Detection of EGFR Gene T790M mutation in Non-Small Cell Lung Cancer using an improved version of the BEAMing technology.2009

    • Author(s)
      Kimiyoshi Nishitani, Kazuya Taniguchi, Jiro Okami, Ken Kodama, Masahiko Higashiyama, kikuya kato
    • Organizer
      第68回日本癌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2009-10-01
    • Related Report
      2009 Annual Research Report
  • [Presentation] Intratumor heterogeneity of epidermal growth factor receptor mutations in lung cancer and its correlation of response to gefitinib2009

    • Author(s)
      Jiro Okami, Kazuya Taniguchi, Masahiko Higashiyama, Jun Maeda, Kazuyuki Oda, Naoki Orita, Kyoko Koizumi, Ken Kodama
    • Organizer
      第13回世界肺癌学会
    • Place of Presentation
      サンフランシスコ
    • Year and Date
      2009-07-31
    • Related Report
      2009 Final Research Report
  • [Presentation] Intratumor heterogeneity of epidermal growth factor receptor mutations in lung cancer and its correlation of response to gefitinib.2009

    • Author(s)
      Jiro Okami, Kazuya Taniguchi, Masahiko Higashiyama, Jun Maeda, Kazuyuki Oda, Naoki Orita, Kyoko Koizumi, Ken Kodama, Kikuya Kato
    • Organizer
      IASLC2009(世界肺癌学会2009)
    • Place of Presentation
      サンフランシスコ
    • Year and Date
      2009-07-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] Genetic and epigenetic aberrations in human multiple hepatocellular carcinoma2009

    • Author(s)
      Kazuya Taniguchi, Terumasa Yamada, Yo Sasaki, Kikuya Kato
    • Organizer
      第100回米国癌学会総会
    • Place of Presentation
      デンバー
    • Year and Date
      2009-04-18
    • Related Report
      2009 Final Research Report
  • [Presentation] Genetic and epigenetic aberrations in human multiple hepato cellular carcinoma.2009

    • Author(s)
      Kazuya Taniguchi, Terumasa Yamada, Yo Sasaki, Kikuya Kato.
    • Organizer
      AACR(米癌学会、第100回総会)
    • Place of Presentation
      コロラド、デンバー
    • Year and Date
      2009-04-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] Evaluation of BEAMing, a sensitive method for mutation detection, and application for diagnosis of drug resistance2008

    • Author(s)
      Moriyama Satomi, Kazuya Taniguchi, Jiro Okami, Ken Kodama, Masahiko Higashiyama, Kikuya Kato
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-28
    • Related Report
      2009 Final Research Report
  • [Presentation] Epigenetic and genetic analysis of multiple hepatocellular carcinoma2008

    • Author(s)
      Kazuya Taniguchi, Kenya Tabuchi, Kazunori Maekawa, Terumasa Yamada, Yo Sasaki, Kikuya Kato
    • Organizer
      第67回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-28
    • Related Report
      2009 Final Research Report
  • [Presentation] Evaluation of BEAMing, a sensitive method for mutation detection, and application for diagnosis of drug resistance2008

    • Author(s)
      Moriyama S, Taniguchi K, et al.
    • Organizer
      第67回 日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 肺癌におけるEGFR遺伝子変異の組織内多様性とその抗癌剤感受性への影響2008

    • Author(s)
      谷口一也、岡見次郎、東山聖彦、児玉憲、加藤菊也
    • Organizer
      第28回分子腫瘍マーカー研究会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-10-27
    • Related Report
      2009 Final Research Report
  • [Presentation] 肺癌におけるEGFR遺伝子変異の組織内多様性とその抗癌剤感受性への影響2008

    • Author(s)
      谷口一也, 他4名
    • Organizer
      第28回分子腫瘍マーカー研究会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2008-10-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Multipoint microsampling for analysis of genetic heterogeneity within cancer tissues2008

    • Author(s)
      Kazuya Taniguchi, Jiro Okami, Ken Kodama, Masahiko Higashiyama, Kikuya Kato
    • Organizer
      第99回米国癌学会総会
    • Place of Presentation
      サンディエゴ
    • Year and Date
      2008-04-16
    • Related Report
      2009 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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