| Project/Area Number |
20F20384
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 45040:Ecology and environment-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
福井 学 (2020-2021) 北海道大学, 低温科学研究所, 教授 (60305414)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI JACKSON・MAKOTO 北海道大学, 低温科学研究所, 博士研究員 (60976207)
TSUJI JACKSON 北海道大学, 低温科学研究所, 外国人特別研究員
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| Project Period (FY) |
2020-11-13 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2022: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2021: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2020: ¥400,000 (Direct Cost: ¥400,000)
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| Keywords | Photosynthesis / Phototrophy / Iron cycling / Metagenomics / Evolution / Chloroflexi / Chloroflexota / Genomics / 光合成 / 微生物 / 進化 |
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| Outline of Research at the Start |
酸素非発生型から酸素発生型光合成への進化は未だ解けない謎である。本研究では、この光合成進化の謎の解明に挑戦する。酸素発生型光合成生物は光合成の中心的な機能である光化学系IとIIを有するが、酸素非発生型はそのどちらかを有する。上記進化を解明する上で鍵を握る新規微生物は嫌気性であるものの、未だ単離培養されていない。そこで、上記微生物の単離培養を目指す。さらに、我が国の部分循環湖の嫌気水層からも同様の微生物の探索を行い、メタゲノム解析並び培養実験等から系統学的および代謝生理学的多様性を明らかにする。以上により、酸素非発生型から酸素発生型光合成への進化プロセスの解明を目指す。
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| Outline of Annual Research Achievements |
In FY2022, we combined field data, bioinformatics, and laboratory experiments to gain new insights into the ecology and physiology of "Candidatus Chlorohelix allophototropha" strain L227-S17, a highly novel phototrophic bacterium. In the field, we revisited Oze National Park (Fukushima-ken) and detected a second L227-17-like population in iron-rich sediment samples. These results demonstrate that L227-S17 relatives are reoccurring and diverse in a local field site and let us plan to cultivate these organisms in future. Although we did not detect L227-S17 relatives in metagenomes we sequenced from an iron-rich lake (Lake Mizugaki, Yamanshi-ken), we recovered novel genomes bins with potential for iron cycling that shed new light on nutrient cycling in iron-rich ecosystems. We are continuing to analyze our Lake Harutori dataset from FY2021. Using bioinformatics, we probed the global diversity of L227-S17 relatives by searching >160,000 publicly available DNA/RNA sequencing datasets. Genome bins recovered from this survey greatly expand the known diversity of the L227-S17 clade and provide a valuable dataset to explore the evolution of photosynthesis. In the laboratory, we obtained compelling preliminary data that the L227-S17 culture performs light-driven iron(II) oxidation, a poorly studied metabolism thought to have been key to nutrient cycling on the early Earth. Altogether, our ecophysiological study of "Ca. Chx. allophototropha" strain L227-S17 provides an important baseline dataset for probing the ancient iron cycle and history of photosynthesis on Earth.
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| Research Progress Status |
令和4年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和4年度が最終年度であるため、記入しない。
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