| Project/Area Number |
20F20402
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 38040:Bioorganic chemistry-related
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
西川 義文 帯広畜産大学, 原虫病研究センター, 教授 (90431395)
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| Co-Investigator(Kenkyū-buntansha) |
ARIEFTA NANANG 帯広畜産大学, 原虫病研究センター, 外国人特別研究員
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| Project Period (FY) |
2020-11-13 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2022: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2021: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2020: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Endophytes / Fungi / Ainu / Anti-Toxoplasma / Antimalaria / Cytotoxicity / 内生菌 / カビ / トキソプラズマ / マラリア / 細胞毒性 |
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| Outline of Research at the Start |
本研究はインドネシア産および日本産植物の内生菌を利用し、インドネシアのヒト及び家畜動物で感染が蔓延しているトキソプラズマ原虫を含む原虫感染症を対象にした大規模な抗原虫活性のスクリーニングを実施する。有望な菌株については、産生化合物の同定を行い、作用機序の解明を進める。植物の内生菌から創薬シーズが発見されれば新たな生物資源価値を提供することができ、原虫病に対する創薬等への応用に必要な学問・技術分野の基盤の育成につながることが期待される。
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| Outline of Annual Research Achievements |
The potent fungi from the screening processes will be applied to the isolation guided by anti-Toxoplasma and antimalaria activities (in vitro). These research steps are intended to confirm and trace the active agent(s) responsible for the anti-Toxoplasma and antimalaria activities. Firstly, each moldy media from scale-up fermentation (600 grams rice) will be extracted using methanol. The dried methanol extract will be suspended in distilled water and partitioned with hexane, chloroform, and ethyl acetate. Each fraction will be evaluated for anti-Toxoplasma activity and antimalaria. The active fraction(s) will be applied to various chromatography methods for further separations. Every step of column chromatography will be guided by anti-Toxoplasma and antimalarial activities, and only the active fractions will be further separated. LC-MS/MS profiling and molecular networking analyses for active fractions will be conducted to generate the compound's map, trace, and visualize the active components' cluster. The structures of isolated active compounds will be determined by spectroscopic methods, including nuclear magnetic resonance (NMR), high-resolution mass spectroscopy (HR-MS), and chemical derivatization. Anti-Toxoplasma and antimalaria effects on growth and cytotoxicity of isolated active compounds will also be evaluated in vitro. The efficacy of the most promising bioactive compounds will be evaluated using the mouse model. Furthermore, the potent fungi will be identified based on the ITS rDNA region.
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| Research Progress Status |
令和4年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和4年度が最終年度であるため、記入しない。
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