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Dynamic monitoring of microvessel models in time and space

Research Project

Project/Area Number 20F20806
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Review Section Basic Section 28050:Nano/micro-systems-related
Research InstitutionThe University of Tokyo

Principal Investigator

松永 行子 (津田行子) (2021-2022)  東京大学, 生産技術研究所, 准教授 (00533663)

Co-Investigator(Kenkyū-buntansha) CACHEUX JEAN  東京大学, 生産技術研究所, 外国人特別研究員
Host Researcher 松永 行子 (津田行子) (2020)  東京大学, 生産技術研究所, 准教授 (00533663)
Foreign Research Fellow CACHEUX JEAN  東京大学, 生産技術研究所, 外国人特別研究員
Project Period (FY) 2020-11-13 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2022: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2021: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2020: ¥300,000 (Direct Cost: ¥300,000)
Keywordsporosity / tissue microenvironment / microdevice / microvessel / permeability / collagen / microfluidics / collgen gel
Outline of Research at the Start

The purpose of the proposed research is to analyze how integrity of a MV (Micro-Vess el) can be maintained or can dysfunction and lead to adverse modulation in permeabil ity. To do so, we aim to use microfluidics and develop an all-in-one solution to observe, stimulate, and characterize perfusable 3D MV in vitro in space and time.

Outline of Annual Research Achievements

Firstly, we went further instrumenting the device with a pressure sensor embedded into an air cavity. We also have developped an 1D analytical model to predict the diffusion of the pressure gradient according to the permeability and elasticity of collagen gel. This unique approach allowed us to show a drop in the permeability and enhanced strain-stiffening of native collagen gels under compression vs. tension, both effects being essentially lost after chemical cross-linking. Further, we report the control of the permeability of native collagen gels using sinusoidal fluid injection, an effect explained by the asymmetric response in tension and compression.

Secondly, we compare the transport of macromolecules through endothelial tissues at mechanical rest or with intraluminal pressure, and correlate these data with electron microscopy of endothelial junctions. Upon application of an intraluminal pressure of 100 Pa, we demonstrate that the flow through the tissue increases by 2.35 times. This increase is associated with a 25% expansion of microvessel diameter, which leads to tissue remodeling and thinning of the paracellular junctions. We recapitulate these data with the deformable monopore model, in which the increase in paracellular transport is explained by the augmentation of the diffusion rate across thinned junctions under mechanical stress.

Research Progress Status

令和4年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和4年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (20 results)

All 2023 2022 2021 Other

All Int'l Joint Research (3 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Open Access: 3 results,  Peer Reviewed: 1 results) Presentation (10 results) (of which Int'l Joint Research: 4 results,  Invited: 8 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Int'l Joint Research] CNRS(フランス)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] LAAS-CNRS(フランス)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] LAAS-CNRS(フランス)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Endothelial tissue remodeling induced by intraluminal pressure enhances paracellular solute transport2023

    • Author(s)
      J. Cacheux, A. Bancaud, D. Alcaide, J.I. Suehiro, Y. Akimoto, H. Sakurai, Y.T. Matsunaga
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2023.01.24.525266

    • Related Report
      2022 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Solute transport in the brain tissue: what are the key biophysical parameters tying in vivo and in vitro studies together?2023

    • Author(s)
      D. Alcaide, J. Cacheux, A. Bancaud, R. Muramatsu, Y. T. Matsunaga
    • Journal Title

      Biomaterials Science

      Volume: - Issue: 10 Pages: 3450-3460

    • DOI

      10.1039/d3bm00027c

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Asymmetry of tensile vs. compressive elasticity and permeability contributes to the regulation of exchanges in collagen gels2023

    • Author(s)
      J. Cacheux, J. Ordonez-Miranda, A. Bancaud, L. Jalabert, M. Nomura, Y.T. Matsunaga
    • Journal Title

      arXiv preprint

      Volume: -

    • Related Report
      2022 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Presentation] Cell-to-cell crosstalk analysis by synthetic approach using microvascular systems2023

    • Author(s)
      松永行子
    • Organizer
      第34回日本臨床微生物学会総会・学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Visualizing angiogenesis and vascular permeability using microvessel model2022

    • Author(s)
      Yukiko T Matsunaga
    • Organizer
      The 22nd International Vascular Biology Meeting: IVBM2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 毛細血管の健康デザイン2022

    • Author(s)
      松永行子
    • Organizer
      第31回日本形成外科学会基礎学術集会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] がん-血管微小環境模倣デバイスによる時空間解析手法の開発2022

    • Author(s)
      松永行子
    • Organizer
      第31回日本がん転移学会学術集会・総会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Cell induced collagen matrix remodeling monitored by interstitial flow metrology2022

    • Author(s)
      Jean Cacheux, Aurelien Bancaud, Jose Ordonez-Miranda, Laurent Jalabert, Yukiko T. Matsunaga
    • Organizer
      Microphysiological systems from organoids to organs-on-chip
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microfluidic-based vascularized microphysiological systems for vascular normalization as therapeutic strategies2021

    • Author(s)
      Yukiko T. Matsunaga
    • Organizer
      MRM2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] マイクロ流体デバイス技術による血管制御機構の理解と展望2021

    • Author(s)
      松永行子
    • Organizer
      第29回日本血管生物医学会学術集会 CVMW2021
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Organ-on-a-chip による三次元微小血管のマルチスケール解析2021

    • Author(s)
      松永行子
    • Organizer
      第43回日本バイオマテリアル学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] マイクロ流体デバイスを用いた構成論的アプローチによる微小血管制御機構の理解2021

    • Author(s)
      松永行子
    • Organizer
      第94回日本生化学学会大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] がん微小環境模倣デバイスによる血管内浸潤挙動の可視化2021

    • Author(s)
      松永行子
    • Organizer
      第80回日本癌学会学術総会 International Sessions 薬剤早期開発の超速化
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] Lab homepage

    • URL

      http://www.matlab.iis.u-tokyo.ac.jp/journals.html

    • Related Report
      2022 Annual Research Report
  • [Remarks] Matsunaga lab, IIS, UTokyo website

    • URL

      http://www.matlab.iis.u-tokyo.ac.jp

    • Related Report
      2020 Annual Research Report
  • [Remarks] LIMMS website

    • URL

      https://limms-tokyo.org

    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] Device and process to measure permeability2022

    • Inventor(s)
      J.Cacheux,A.Bancaud,Y.Matsunaga
    • Industrial Property Rights Holder
      J.Cacheux,A.Bancaud,Y.Matsunaga
    • Industrial Property Rights Type
      特許
    • Filing Date
      2022
    • Related Report
      2021 Annual Research Report
    • Overseas

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Published: 2020-11-16   Modified: 2024-12-25  

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