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Analysis of Anthropocene with biological archives: A case study in Lake Biwa

Research Project

Project/Area Number 20H00208
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 17:Earth and planetary science and related fields
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

Ohkouchi Naohiko  国立研究開発法人海洋研究開発機構, 海洋機能利用部門, 部門長 (00281832)

Co-Investigator(Kenkyū-buntansha) 高津 文人  国立研究開発法人国立環境研究所, 地域環境保全領域, 室長 (30514327)
小川 奈々子  国立研究開発法人海洋研究開発機構, 海洋機能利用部門(生物地球化学センター), グループリーダー (80359174)
石川 尚人  国立研究開発法人海洋研究開発機構, 海洋機能利用部門(生物地球化学センター), 副主任研究員 (80609389)
木庭 啓介  京都大学, 生態学研究センター, 教授 (90311745)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥43,810,000 (Direct Cost: ¥33,700,000、Indirect Cost: ¥10,110,000)
Fiscal Year 2022: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2021: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2020: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Keywords富栄養化 / 琵琶湖 / アミノ酸 / 窒素同位体比 / アーカイブ試料 / 同位体比 / 人新世 / 生物アーカイブ
Outline of Research at the Start

地表面に降り注ぐ太陽エネルギーの一部は,食物連鎖を通じて生物界を流れている。食物連鎖は,自然界における重要な物質循環プロセスであるがゆえ,その詳細な解明は地球表層の環境科学にとって必須の知見である。しかし,人新世に人類活動の影響を大きく受けた水界環境の実状には不明な点が数多く残されている。本研究では,アミノ酸の窒素・炭素同位体比を用いる方法論を最適化し,人類活動の影響を大きく受けた琵琶湖で20世紀半ば以降採取されてきた生物アーカイブ試料に適用し,食物連鎖と人類活動の詳細な解明を目指す。世界の研究機関に保存されている生物アーカイブを,環境変動の記録媒体として有効利用する道筋をつけるものでもある。

Outline of Final Research Achievements

In this study, two new methodologies for analyzing nitrogen isotope ratios of amino acids in living organisms were established and applied to various fish species formerly collected in Lake Biwa and preserved for a long time by formalin fixation. The difference in nitrogen isotope ratios of phenylalanine and methionine was clearly larger than the value (~-5‰) considered to be 100% of the aquatic resources by previous studies, and was considered to be caused by the different distribution of 15N to each amino acid in each species. In this study, we also estimated the trophic levels of various fish species in Lake Biwa using nitrogen isotope ratios of amino acids, and obtained results that corroborate previous ecological observations. We also established a method for measuring carbon isotope ratios of the 16 amino acids that make up proteins.

Academic Significance and Societal Importance of the Research Achievements

本研究は,20世紀半ば以降の琵琶湖を例に,複雑な食物連鎖とその人類活動による影響の解明を目的としている。人類活動を重要なgeological forceとみなす人新世の理解に,特にホルマリン固定された生物アーカイブ試料というこれまでほとんど利用されてこなかった材料に着目する点に学術的意義がある。世界中に存在する生物アーカイブがもし利用可能であることが明らかになれば,巨大なアーカイブの潜在的可能性を大きく広げることになり,富栄養化がローカルな生態系に及ぼす影響という人類が直面する社会問題の原因解明に大きなインパクトを与えるだろう。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Comments on the Screening Results   Annual Research Report
  • Research Products

    (22 results)

All 2022 2021 2020 Other

All Journal Article (10 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 10 results,  Open Access: 10 results) Presentation (10 results) (of which Int'l Joint Research: 8 results,  Invited: 4 results) Remarks (2 results)

  • [Journal Article] Compound-Specific Nitrogen Isotope Analysis of Amino Acids in Eye Lenses as a New Tool to Reconstruct the Geographic and Trophic Histories of Fish2022

    • Author(s)
      Harada Y, Ito S, Ogawa NO, Yoshikawa C, Ishikawa NF, Yoneda M and Ohkouchi N
    • Journal Title

      Front. Mar. Sci

      Volume: 8:796532 Pages: 796532-796532

    • DOI

      10.3389/fmars.2021.796532

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Integrative assessment for amino acid nitrogen isotopic composition in biological tissue samples determined by GC/C/IRMS, LC x EA/IRMS, and LC x GC/C/IRMS.2022

    • Author(s)
      Ishikawa, N. F., Ogawa, N. O., Sun, Y., Chikaraishi, Y., Takano, Y., and Ohkouchi, N.
    • Journal Title

      Limnology and Oceanography, Method

      Volume: 20 Issue: 9 Pages: 531-542

    • DOI

      10.1002/lom3.10502

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Abyssal fauna, benthic microbes, and organic matter quality across a range of trophic conditions in the western Pacific ocean2021

    • Author(s)
      Nomaki Hidetaka、Rastelli Eugenio、Alves Andreia、Suga Hisami、Ramos Sandra、Kitahashi Tomo、Tsuchiya Masashi、Ogawa Nanako O.、Matsui Yohei、Seike Koji、Miyamoto Norio、Corinaldesi Cinzia、Manea Elisabetta、Ohkouchi Naohiko、Danovaro Roberto、Nunoura Takuro、Amaro Teresa
    • Journal Title

      Progress in Oceanography

      Volume: 195 Pages: 102591-102591

    • DOI

      10.1016/j.pocean.2021.102591

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Influences of ocean currents on the diets of demersal fish communities in the western North Pacific revealed by their muscle carbon and nitrogen isotopic compositions2021

    • Author(s)
      Naoto F Ishikawa, Nanako O Ogawa, Yoshito Chikaraishi, Moto-omi Yamaguchi, Katsunori Fujikura, Yosuke Miyairi, Yusuke Yokoyama, Toshi Nagata, Naohiko Ohkouchi
    • Journal Title

      Frontiers in Marine Science

      Volume: 8 Pages: 465-465

    • DOI

      10.3389/fmars.2021.641282

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Analytical development of seamless procedures on cation-exchange chromatography and ion-pair chromatography with high-precision mass spectrometry for short-chain peptides.2021

    • Author(s)
      Takano, Y., Oba, Y., Furota, S., Naraoka, H., Ogawa, N., Blattmann, T. M. and Ohkouchi, N.
    • Journal Title

      Inter. J. Mass Spectrom.

      Volume: 462 Pages: 116529-116529

    • DOI

      10.1016/j.ijms.2021.116529

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Trophic hierarchy of coastal marine fish communities viewed via compound-specific isotopic analysis of amino acids.2020

    • Author(s)
      Xing, D., Choi, B., Takizawa, Y., Fan, R., Sugaya, S., Tsuchiya, M., Ohkouchi, N., and Chikaraishi, Y.
    • Journal Title

      Marine Ecology Progress Series

      Volume: 652 Pages: 137-142

    • DOI

      10.3354/meps13475

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] New insights into isotopic fractionation for central metabolism and biosynthesis in organisms: based on 15N/14N and 13C/12.C of amino acids from four consumer-diet combinations.2020

    • Author(s)
      Takizawa, Y., Takano, Y., Choi, B., Dharampal, P. S., Steffan, S. A., Ogawa, N. O., Ohkouchi, N., Wada, E., and Chikaraishi, Y.
    • Journal Title

      Progress in Earth and Planetary Science

      Volume: 7 Issue: 1 Pages: 1-13

    • DOI

      10.1186/s40645-020-00364-w

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A method for stable carbon isotope measurement of underivatized individual amino acids by multi‐dimensional high‐performance liquid chromatography and elemental analyzer/isotope ratio mass spectrometry2020

    • Author(s)
      Sun Yuchen、Ishikawa Naoto F.、Ogawa Nanako O.、Kawahata Hodaka、Takano Yoshinori、Ohkouchi Naohiko
    • Journal Title

      Rapid Communications in Mass Spectrometry

      Volume: 34 Issue: 20

    • DOI

      10.1002/rcm.8885

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Combined use of radiocarbon and stable carbon isotopes for the source mixing model in a stream food web.2020

    • Author(s)
      Ishikawa, N. F., Finlay, J. C., Uno, H., Ogawa, N. O., Ohkouchi, N., Tayasu, I., and Power, M. E.
    • Journal Title

      Limnology and Oceanography Letters

      Volume: 65 Pages: 2688-2696

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Tracking long-distance migration of marine fishes using compound-specific stable isotope analysis of amino acids2020

    • Author(s)
      Matsubayashi J., Osada Y., Tadokoro K., Abe Y., Yamaguchi A., Shirai K., Honda K., Yoshikawa C., Ogawa N. O., Ohkouchi N., Ishikawa N. F., Nagata T., Miyamoto H., Nishino S., Tayasu I.
    • Journal Title

      Ecology Letters

      Volume: なし Issue: 5 Pages: 881-890

    • DOI

      10.1111/ele.13496

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 同位体生態学 ver. 2.1:アミノ酸窒素同位体比,さらにその先へ2022

    • Author(s)
      大河内直彦
    • Organizer
      同位体環境学シンポジウム
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Amino acid nitrogen isotope ratio as a tool for ecological study2022

    • Author(s)
      N. Ohkouchi
    • Organizer
      8th China National Symposium of Stable Isotope Ecology
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Development and applications of a liquid chromatography method for the compound- and enantiomer-specific stable carbon isotope analysis of amino acids in environmental samples2022

    • Author(s)
      Y. Sun, N. F. Ishikawa, N. O.O gawa, Y.Takano, N. Ohkouchi
    • Organizer
      Ocean Sciences Meeting 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microbial Reworking of Organic Matter in Marine Sediments Revealed by Carbon Isotopic Compositions of Individual Amino Acids2022

    • Author(s)
      Y. Sun, N. F. Ishikawa, N. O. Ogawa, J. Kuroda, Y.Takano, N. Ohkouchi
    • Organizer
      日本地球化学会 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development and applications of a liquid chromatography method for the compound- and enantiomer-specific stable carbon isotope analysis of amino acids in environmental samples2021

    • Author(s)
      Yuchen Sun, Naoto F. Ishikawa, Thomas M. Blattmann, Nanako O. Ogawa, Hodaka Kawahata, Yoshinori Takano, Naohiko Ohkouchi
    • Organizer
      Ocean Science Meeting 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 水晶体の同位体分析による魚類の回遊経路復元手法の開発2021

    • Author(s)
      原田洋太,伊藤進一,小川奈々子,吉川千里,石川尚人,大河内直彦
    • Organizer
      JpGU 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nitrogen isotope mapping in the North Pacific using a marine nitrogen isotope model2021

    • Author(s)
      Chisato Yoshikawa, Masahito Shigemitsu, Akitomo Yamamoto, Akira Oka, Naohiko Ohkouchi
    • Organizer
      JpGU 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sub-microgram sulfur isotope analysis for the biological, geological, and extraterrestrial samples by sensitivity-improved EA/IRMS2021

    • Author(s)
      Nanako O. Ogawa, Toshihiro Yoshimura, Takeru Kochi, Junichiro Kuroda, Yoshinori Takano, Naohiko Ohkouch
    • Organizer
      JpGU 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 同位体で解く生態系2021

    • Author(s)
      大河内直彦
    • Organizer
      西アジア考古学会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] High-precision compound-specific carbon isotopic analysis of underivatized amino acids using a multidimensional-HPLC and nano-EA/IRMS2020

    • Author(s)
      Yuchen Sun, Naoto F. Ishikawa, Nanako O.Ogawa, Hodaka Kawahata, Yoshinori Takano, Naohiko Ohkouchi
    • Organizer
      Eauropean Geoscience Union 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Remarks]

    • URL

      https://www.jamstec.go.jp/biogeochem/

    • Related Report
      2022 Annual Research Report
  • [Remarks] 海洋研究開発機構 生物地球化学センター 大河内直彦

    • URL

      https://www.jamstec.go.jp/biogeochem/member/ohkouchi/index.html

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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