| Budget Amount *help |
¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000)
Fiscal Year 2023: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2022: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2021: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2020: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Outline of Final Research Achievements |
High-speed AFM (HS-AFM) is a unique microscope that allows simultaneous observation of the shape and motion of biomolecules in action. However, many life phenomena cannot be observed with the conventional temporal resolution of HS-AFM. Here, in order to observe a wider range of life phenomena with HS-AFM, we significantly improved the response speed of the Z-scanner, amplitude-detector for cantilever oscillation and cantilever, which had limited the temporal resolution of the HS-AFM. Using the HS-AFM containing the devices developed here, we succeeded in observing the process of assembly of translation factors into ribosomal stalk complexes and the long-range cleavage of DNA by CRISPR-Cas3 while reeling DNA, and thus obtained new biologically meaningful findings.
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