| Budget Amount *help |
¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000)
Fiscal Year 2023: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2022: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2021: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2020: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we developed an atomic force microscopy (AFM) technique for high-resolution observation of living cell surfaces. In this technique, cells are cultured on a silicon nitride thin film with many pores of several microns in diameter, and the cell basal surface exposed through the pores is observed by AFM. This suppresses cell membrane fluctuation and molecular diffusion within the cell membrane, enabling molecular-scale (< 10 nm) observation. Furthermore, by comparing the surface structures of live and chemically fixed cells at the nanoscale using this technique, it was found that the commonly used chemical fixation method results in aggregation of proteins, which significantly alters the cell surface structure at the molecular scale. Other findings include the effect of MET receptors on cell surface structure.
Translated with DeepL.com (free version)
|
