Novel role of Golgi apparatus for non-cell autonomous events
Project/Area Number |
20H00467
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
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Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
Shimizu Shigeomi 東京医科歯科大学, 難治疾患研究所, 教授 (70271020)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Project Status |
Completed (Fiscal Year 2022)
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Budget Amount *help |
¥45,630,000 (Direct Cost: ¥35,100,000、Indirect Cost: ¥10,530,000)
Fiscal Year 2022: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2021: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2020: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
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Keywords | GOMED / ゴルジ体 / オートファジー / Flad / Ulk1 / PI4P / タンパク質分解 / 細胞生物 / 小胞輸送 |
Outline of Research at the Start |
ゴルジ体はこれまで、「分子に修飾を加えることによって、細胞内外の適切な運搬場所を示す」オルガネラであると理解されてきました。しかしながら、私たちは、ゴルジ体の新たな機能として、蛋白質分解を行なっていることを発見しました。これは、ゴルジ体に新たな役割があることを意味しています。本研究では、このゴルジ体の新たな機能に関して、その実態や生物学的な役割を明らかにします。
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Outline of Final Research Achievements |
GOMED is a cellular function in which trans-Golgi membranes are greatly deformed to encapsulate proteins to be degraded and subsequently fuse with lysosomes to degrade their contents. In the "Elucidation of GOMED execution mechanism," we identified the Wipi3 molecule as the executor molecule and found that phosphorylation of Ulk1 is important in the initial reaction. In "Visualization of GOMED," we developed a new GOMED analysis method, the FLAD method. In "Physiological Functions of GOMED," we found that GOMED contributes to the maintenance of cerebellar cell functions.
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Academic Significance and Societal Importance of the Research Achievements |
ゴルジ体はこれまで、小胞体から運ばれてきた分子に、糖鎖修飾、リン酸化修飾などの適切な翻訳後修飾を加えることによって、各分子を細胞外、細胞膜、リソソームなどに適切に運搬するオルガネラとして考えられてきた。しかしながら、研究代表者は、Golgi-mediated degradation pathway (GOMED)と命名した細胞機能により、ゴルジ体が、周囲の環境に応じて、細胞外分泌の分泌量や細胞膜レセプターの種類や量を巧妙に調節していることを見出した。
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Report
(5 results)
Research Products
(20 results)
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[Journal Article] Absence of ULK1 decreases AMPK activity in the kidney, leading to chronic kidney disease progression2023
Author(s)
Tomoki Yanagi, Hiroaki Kikuchi, Koichiro Susa, Naohiro Takahashi, Naohiro Takahashi, Hiroki Bamba, Takefumi Suzuki, Yuta Nakano, Tamami Fujiki, Yutaro Mori, Fumiaki Ando, Shintaro Mandai, Takayasu Mori, Koh Takeuchi, Shinya Honda, Satoru Torii, Shigeomi Shimizu, Tatemitsu Rai, Shinichi Uchida, Eisei Sohara
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Journal Title
Genes to Cells
Volume: 28
Issue: 1
Pages: 5-14
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Nickel particles are present in Crohn's disease tissue and exacerbate intestinal inflammation in IBD susceptible mice.2022
Author(s)
Hiroki Matsuda, Yoichi Nibe-Shirakihara, Akiko Tamura, Emi Aonuma, Satoko Arakawa, Kana Otsubo, Yasuhiro Nemoto, Takashi Nagaishi, Kiichiro Tsuchiya, Shigeomi Shimizu, Averil Ma, Mamoru Watanabe, Motohiro Uo, Ryuichi Okamoto, Shigeru Oshima.
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Journal Title
Biochemical and Biophysical Research Communications.
Volume: 592
Pages: 74-80
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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