| Budget Amount *help |
¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000)
Fiscal Year 2022: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2021: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2020: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
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| Outline of Final Research Achievements |
This study applied advanced technologies to analyze the nano-scale structure and molecular distribution within synapses and established a model which explains the relationship between molecular dynamics and the function of spine synapses. We further established a method measuring characteristic synapse structure in the neural circuit of living animal models. From the nano-scale structural analysis of spine synapses in culture neurons, we could provide a quantitative model explaining the contribution of spine head curvature in synapse stabilization. Furthermore, the expected spine morphological changes could be analyzed both in culture preparations and in vivo by taking advantage of tissue-clearing technology and expansion microscopy. The obtained imaging data support the existence of common features of neurons in two different environments.
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