SIK3および新規睡眠制御分子による睡眠制御シグナルカスケードの解明

• Project/Area Number: 20H00567
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
• Research Institution: University of Tsukuba
• Principal Investigator: 船戸 弘正 筑波大学, 国際統合睡眠医科学研究機構, 客員教授 (90363118)
• Co-Investigator(Kenkyū-buntansha): 三好 千香 東邦大学, 医学部, 博士研究員 (60613437) ; 成清 公弥 東邦大学, 医学部, 助教 (70599836) ; 山形 朋子 東邦大学, 医学部, 助教 (90584433)
• Project Period (FY): 2020-04-01 – 2025-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000); Fiscal Year 2023: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2022: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2021: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2020: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 睡眠 / 遺伝子改変マウス / リン酸化酵素 / 細胞内シグナル / 睡眠覚醒 / りん酸化酵素

Outline of Research at the Start

睡眠は動物に普遍的に認められ心身の健康に必須である。しかし、睡眠制御の分子機構は明らかになっていない。研究代表者らは新しい睡眠制御分子としてリン酸化酵素SIK3を同定しSIK3が睡眠必要量を規定する分子機構を構成していることを明らかにした。SIK1およびSIK2も同様に役割を持つことを明らかにしつつある。本研究ではSIK3がその下流分子と協働して睡眠必要量を決める仕組みを最新の知見と技術を用いて明らかにする。

Outline of Annual Research Achievements

睡眠覚醒制御の実行回路を構成する神経細胞の働きを変化させる細胞内の仕組みは明らかではない。研究代表者らは順遺伝学的アプローチにより新しい睡眠制御分子、特に睡眠要求を調整する役割を持つ分子としてリン酸化酵素SIK3を同定した。Sleepy変異型のSIK3を発現すると睡眠が増大する。睡眠制御におけるSIK3の基質の同定が課題となっていた。睡眠の表現型から機能獲得型変異と想定されていたが、精製した蛋白質を用いるin vitro kinase アッセイでは機能獲得型の変化は認められなかった。培養細胞を用いたHDAC4を介した転写アッセイでは機能獲得型変化を示したことから、睡眠の変化もHDAC4を介したものと理解できる。HDAC4の睡眠における役割の一端を前年度に公表したが、さらに検討を続けておりClass II HDACの代償性や発現部位の違いも考慮し、発生期や末梢組織の影響を除外することで睡眠制御の分子機構が徐々に明らかになってきた。睡眠覚醒行動に密接な関係を持つ概日リズム行動についてもHDAC4が関与することを、HDAC4ヘテロ欠損マウスを用いることで示すことができた。HDAC４ヘテロ欠損マウスは恒暗条件での概日リズム長が野生型マウスよりも短縮していた。SIK3欠損とは逆向きの変化であり、SIK3がHDAC4をリン酸化することでHDAC4の核内への移行を阻害しHDAC4の転写抑制を制限することで統一的に理解できる。HDAC4ヘテロ欠損マウスは暗期初期の覚醒開始時間に野生型マウスとの差異を示さなかったが、明期側への前倒しは光のマスク効果のために検出できにくいためだろう。このように、HDAC4が睡眠及び概日リズムにおいて重要な役割を果たしていると示すことができた。

Research Progress Status

令和5年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和5年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] 北京生命科学研究所(中国)
• [Int'l Joint Research] NIBS(中国)
• [Journal Article] Whole Brain Mapping of Orexin Receptor mRNA Expression Visualized by Branched In Situ Hybridization Chain Reaction (2024)
  • Author(s): Tsuneoka Yousuke、Funato Hiromasa
  • Journal Title: eneuro Volume: 11 Pages: ENEURO.0474-23.2024
  • DOI: 10.1523/eneuro.0474-23.2024
  • Peer Reviewed / Open Access
• [Journal Article] Microglia modulate sleep/wakefulness under baseline conditions and under acute social defeat stress in adult mice (2023)
  • Author(s): Miyanishi Kazuya、Hotta-Hirashima Noriko、Miyoshi Chika、Hayakawa Satsuki、Kakizaki Miyo、Kanno Satomi、Ikkyu Aya、Funato Hiromasa、Yanagisawa Masashi
  • Journal Title: Neuroscience Research Volume: in press Pages: 8-19
  • DOI: 10.1016/j.neures.2023.11.010
  • Peer Reviewed / Open Access
• [Journal Article] A phosphorylation-deficient mutant of Sik3, a homolog of Sleepy, alters circadian sleep regulation by PDF neurons in Drosophila (2023)
  • Author(s): Kobayashi Riho、Nakane Shin、Tomita Jun、Funato Hiromasa、Yanagisawa Masashi、Kume Kazuhiko
  • Journal Title: Frontiers in Neuroscience Volume: 17 Pages: 1-10
  • DOI: 10.3389/fnins.2023.1181555
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of TRPM8-expressing neurons in the adult mouse hypothalamus (2023)
  • Author(s): Tsuneoka Yousuke、Nishikawa Taichi、Furube Eriko、Okamoto Kaho、Yoshimura Ryoichi、Funato Hiromasa、Miyata Seiji
  • Journal Title: Neuroscience Letters Volume: 814 Pages: 137463-137463
  • DOI: 10.1016/j.neulet.2023.137463
  • Peer Reviewed
• [Journal Article] Face validation and pharmacologic analysis of Sik3 mutant mouse as a possible model of idiopathic hypersomnia (2023)
  • Author(s): Elhosainy Asmaa、Suzuki-Abe Haruka、Kaushik Mahesh K.、Kim Staci J.、Saitoh Tsuyoshi、Ishikawa Yukiko、Hotta-Hirashima Noriko、Miyoshi Chika、Funato Hiromasa、Yanagisawa Masashi
  • Journal Title: European Journal of Pharmacology Volume: 954 Pages: 175877-175877
  • DOI: 10.1016/j.ejphar.2023.175877
  • Peer Reviewed / Open Access
• [Journal Article] SIK3-HDAC4 in the suprachiasmatic nucleus regulates the timing of arousal at the dark onset and circadian period in mice (2023)
  • Author(s): Asano Fuyuki、Kim Staci J.、Fujiyama Tomoyuki、Miyoshi Chika、Hotta-Hirashima Noriko、Asama Nodoka、Iwasaki Kanako、Kakizaki Miyo、Mizuno Seiya、Mieda Michihiro、Sugiyama Fumihiro、Takahashi Satoru、Shi Shoi、Hirano Arisa、Funato Hiromasa、Yanagisawa Masashi
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 120 Issue: 11 Pages: 1-11
  • DOI: 10.1073/pnas.2218209120
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex (2023)
  • Author(s): Oda Satoko、Funato Hiromasa
  • Journal Title: Frontiers in Neuroanatomy Volume: 17 Pages: 1111008-1111008
  • DOI: 10.3389/fnana.2023.1111008
  • Peer Reviewed / Open Access
• [Journal Article] A signalling pathway for transcriptional regulation of sleep amount in mice (2022)
  • Author(s): Zhou Rui、Wang Guodong、Li Qi、Chen Zhiyu、Chen Lin、Shi Bihan、Wang Haiyan、Wang Xia、Li Huaiye、Cai Tao、Li Bin、Wang Fengchao、Funato Hiromasa、Yanagisawa Masashi、Zhang Eric Erquan、Liu Qinghua
  • Journal Title: Nature Volume: 612 Issue: 7940 Pages: 519-527
  • DOI: 10.1038/s41586-022-05510-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Kinase signalling in excitatory neurons regulates sleep quantity and depth (2022)
  • Author(s): Kim Staci J.、Miyoshi Chika、Yanagisawa Masashi、Funato Hiromasa、et., al.
  • Journal Title: Nature Volume: 612 Issue: 7940 Pages: 512-518
  • DOI: 10.1038/s41586-022-05450-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization (2022)
  • Author(s): Tsuneoka Yousuke、Atsumi Yusuke、Makanae Aki、Yashiro Mitsuru、Funato Hiromasa
  • Journal Title: Frontiers in Molecular Neuroscience Volume: 15 Pages: 976349-976349
  • DOI: 10.3389/fnmol.2022.976349
  • Peer Reviewed / Open Access
• [Journal Article] IκBζ controls IL-17-triggered gene expression program in intestinal epithelial cells that restricts colonization of SFB and prevents Th17-associated pathologies (2022)
  • Author(s): Yamazaki Soh、Inohara Naohiro、Ohmuraya Masaki、Tsuneoka Yousuke、Yagita Hideo、Katagiri Takaharu、Nishina Takashi、Mikami Tetuo、Funato Hiromasa、Araki Kimi、Nakano Hiroyasu
  • Journal Title: Mucosal Immunology Volume: 15 Issue: 6 Pages: 1321-1337
  • DOI: 10.1038/s41385-022-00554-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] OX2R-selective orexin agonism is sufficient to ameliorate cataplexy and sleep/wake fragmentation without inducing drug-seeking behavior in mouse model of nalcolepsy (2022)
  • Author(s): Yamamoto H, Nagumo Y, Ishikawa Y, Irukayama-Tomobe Y, Namekawa Y, Nemoto T, Tanaka H, Takahashi G, Tokuda A, Saitoh T, Nagase H, Funato H, Yanagisawa M.
  • Journal Title: PLos One Volume: 17 Issue: 7 Pages: e0271901-e0271901
  • DOI: 10.1371/journal.pone.0271901
  • Peer Reviewed / Open Access
• [Journal Article] Hunt for mammalian sleep-regulating genes (2022)
  • Author(s): Funato Hiromasa、Yanagisawa Masashi
  • Journal Title: Brain Science Advances Volume: 8 Issue: 3 Pages: 173-182
  • DOI: 10.26599/bsa.2022.9050012
  • Peer Reviewed / Open Access
• [Journal Article] Mice Lacking Cerebellar Cortex and Related Structures Show a Decrease in Slow-Wave Activity With Normal Non-REM Sleep Amount and Sleep Homeostasis (2022)
  • Author(s): Fujiyama Tomoyuki、Takenaka Henri、Asano Fuyuki、Miyanishi Kazuya、Hotta-Hirashima Noriko、Ishikawa Yukiko、Kanno Satomi、Seoane-Collazo Patricia、Miwa Hideki、Hoshino Mikio、Yanagisawa Masashi、Funato Hiromasa
  • Journal Title: Frontiers in Behavioral Neuroscience Volume: 16 Pages: 910461-910461
  • DOI: 10.3389/fnbeh.2022.910461
  • Peer Reviewed / Open Access
• [Journal Article] Metabolomic and pharmacologic analyses of brain substances associated with sleep pressure in mice (2022)
  • Author(s): Suzuki-Abe Haruka、Sonomura Kazuhiro、Nakata Shinya、Miyanishi Kazuya、Mahmoud Asmaa、Hotta-Hirashima Noriko、Miyoshi Chika、Sato Taka-Aki、Funato Hiromasa、Yanagisawa Masashi
  • Journal Title: Neuroscience Research Volume: 177 Pages: 16-24
  • DOI: 10.1016/j.neures.2021.11.008
  • Peer Reviewed / Open Access
• [Journal Article] Protocol for sleep analysis in the brain of genetically modified adult mice (2021)
  • Author(s): Kanako Iwasaki, Noriko Hotta-Hirashima, Hiromasa Funato, Masashi Yanagisawa
  • Journal Title: STAR Protocols Volume: 2 Issue: 4 Pages: 100982-100982
  • DOI: 10.1016/j.xpro.2021.100982
  • Peer Reviewed / Open Access
• [Journal Article] The Role of Reproductive Hormones in Sex Differences in Sleep Homeostasis and Arousal Response in Mice (2021)
  • Author(s): Choi Jinhwan、Kim Staci J.、Fujiyama Tomoyuki、Miyoshi Chika、Park Minjeong、Suzuki-Abe Haruka、Yanagisawa Masashi、Funato Hiromasa
  • Journal Title: Frontiers in Neuroscience Volume: 15 Pages: 739236-739236
  • DOI: 10.3389/fnins.2021.739236
  • Peer Reviewed / Open Access
• [Journal Article] Physical contact in parent-infant relationship and its effect on fostering a feeling of safety (2021)
  • Author(s): Yoshida Sachine、Funato Hiromasa
  • Journal Title: iScience Volume: 24 Issue: 7 Pages: 102721-102721
  • DOI: 10.1016/j.isci.2021.102721
  • Peer Reviewed / Open Access
• [Journal Article] Two novel mouse models mimicking minor deletions in 22q11.2 deletion syndrome revealed the contribution of each deleted region to psychiatric disorders (2021)
  • Author(s): Saito Ryo、Miyoshi Chika、Koebis Michinori、Kushima Itaru、Nakao Kazuki、Mori Daisuke、Ozaki Norio、Funato Hiromasa、Yanagisawa Masashi、Aiba Atsu
  • Journal Title: Molecular Brain Volume: 14 Issue: 1 Pages: 68-68
  • DOI: 10.1186/s13041-021-00778-7
  • Peer Reviewed / Open Access
• [Journal Article] Cellular Composition of the Preoptic Area Regulating Sleep, Parental, and Sexual Behavior (2021)
  • Author(s): Tsuneoka Yousuke、Funato Hiromasa
  • Journal Title: Frontiers in Neuroscience Volume: 15 Pages: 1-15
  • DOI: 10.3389/fnins.2021.649159
  • Peer Reviewed / Open Access
• [Journal Article] Induction of Mutant Sik3Sleepy Allele in Neurons in Late Infancy Increases Sleep Need (2021)
  • Author(s): Iwasaki Kanako, Fujiyama Tomoyuki, Nakata Shinya, Park Minjeong, Miyoshi Chika, Hotta-Hirashima Noriko, Ikkyu Aya, Kakizaki Miyo, Sugiyama Fumihiro, Mizuno Seiya, Abe Manabu, Sakimura Kenji, Takahashi Satoru, Funato Hiromasa, Yanagisawa Masashi
  • Journal Title: The Journal of Neuroscience Volume: 41 Issue: 12 Pages: 2733-2746
  • DOI: 10.1523/jneurosci.1004-20.2020
  • Peer Reviewed / Open Access
• [Journal Article] Generation of a p16 Reporter Mouse and Its Use to Characterize and Target p16high Cells In Vivo. (2020)
  • Author(s): Omori S, Wang TW, Johmura Y, ..., Sakamoto T (15番目), ..., Nakanishi M.
  • Journal Title: Cell Metab Volume: 32 Issue: 5 Pages: 814-828
  • DOI: 10.1016/j.cmet.2020.09.006
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sleep Architecture in Mice Is Shaped by the Transcription Factor AP-2β (2020)
  • Author(s): Nakai Ayaka、Fujiyama Tomoyuki、Nagata Nanae、Kashiwagi Mitsuaki、Ikkyu Aya、Takagi Marina、Tatsuzawa Chika、Tanaka Kaeko、Kakizaki Miyo、Kanuka Mika、Kawano Taizo、Mizuno Seiya、Sugiyama Fumihiro、Takahashi Satoru、Funato Hiromasa、Sakurai Takeshi、Yanagisawa Masashi、Hayashi Yu
  • Journal Title: Genetics Volume: 216 Issue: 3 Pages: 753-764
  • DOI: 10.1534/genetics.120.303435
  • Peer Reviewed
• [Journal Article] Hypnotic effect of thalidomide is independent of teratogenic ubiquitin/proteasome pathway (2020)
  • Author(s): Hirose Yuki、Kitazono Tomohiro、Sezaki Maiko、Abe Manabu、Sakimura Kenji、Funato Hiromasa、Handa Hiroshi、Vogt Kaspar E.、Yanagisawa Masashi
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 117 Issue: 37 Pages: 23106-23112
  • DOI: 10.1073/pnas.1917701117
  • Peer Reviewed
• [Journal Article] Ablation of Ventral Midbrain/Pons GABA Neurons Induces Mania-like Behaviors with Altered Sleep Homeostasis and Dopamine D2R-mediated Sleep Reduction (2020)
  • Author(s): Honda Takato, Takata Yohko, Cherasse Yoan, Mizuno Seiya, Sugiyama Fumihiro, Takahashi Satoru, Funato Hiromasa, Yanagisawa Masashi, Lazarus Michael, Oishi Yo
  • Journal Title: iScience Volume: 23 Issue: 6 Pages: 101240-101240
  • DOI: 10.1016/j.isci.2020.101240
  • NAID: 120007163338
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Loss of the conserved PKA sites of SIK1 and SIK2 increases sleep need (2020)
  • Author(s): Park Minjeong, Miyoshi Chika, Fujiyama Tomoyuki, Kakizaki Miyo, Ikkyu Aya, Honda Takato, Choi Jinhwan, Asano Fuyuki, Mizuno Seiya, Takahashi Satoru, Yanagisawa Masashi, Funato Hiromasa
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 8676-8676
  • DOI: 10.1038/s41598-020-65647-0
  • NAID: 120007163335
  • Peer Reviewed / Open Access
• [Journal Article] Modified in situ Hybridization Chain Reaction Using Short Hairpin DNAs (2020)
  • Author(s): Tsuneoka Yousuke、Funato Hiromasa
  • Journal Title: Frontiers in Molecular Neuroscience Volume: 13 Pages: 75-75
  • DOI: 10.3389/fnmol.2020.00075
  • Peer Reviewed / Open Access
• [Presentation] SIK3 variants regulate sleep (2024)
  • Author(s): Hiromasa Funato
  • Organizer: 第101回日本生理学会
  • Invited
• [Presentation] Kinase signalling pathway regulates NREM sleep (2024)
  • Author(s): Hiromasa Funato
  • Organizer: Gordon Research Conference "Sleep Regulation and Function"
  • Int'l Joint Research
• [Presentation] 睡眠の質と量を制御する細胞内シグナル伝達 (2023)
  • Author(s): 船戸弘正
  • Organizer: 第34回 高遠分子細胞生物学シンポジウム
  • Invited
• [Presentation] 食欲と睡眠欲の中枢 (2023)
  • Author(s): 船戸弘正
  • Organizer: 第64回日本心身医学会総会
  • Invited
• [Presentation] Branched HCR法によって明らかになったマウス脳内オレキシン受容体mRNAの分布 (2023)
  • Author(s): 恒岡洋右, 船戸弘正
  • Organizer: 日本解剖学会
• [Presentation] 睡眠量の規定における出生後の神経細胞での転写因子TFAP2Bの役割 (2023)
  • Author(s): 中井 彩加 、柏木 光昭、藤山 知之、岩崎 加奈子、船戸 弘正、柳沢 正史、櫻井 武、林 悠
  • Organizer: 第100回日本生理学会
• [Presentation] Kinase signaling pathway regulating non-REM sleep (2023)
  • Author(s): 船戸弘正
  • Organizer: 第100回日本生理学会
• [Presentation] Social buffering of isolation by somatosensory stimulation in mouse pups (2022)
  • Author(s): Sachine Yoshida, Hiromasa Funato
  • Organizer: Society for Neuroscience 2022
• [Presentation] Sik3 regulates circadian rhythms and sleep need through distinct neurons and brain regions. (2022)
  • Author(s): Fuyuki Asano, Tomoyuki Fujiyama, Chika Miyoshi, Tomohiro Kitazono, Staci J. Kim, Kanako Iwasaki, Noriko Hotta-Hirashima, Miyo Kakizaki, Aya Ikkyu, Satomi Kanno, Shinya Nakata, Nodoka Asama, Takumi Tsukamoto, Arisa Hirano, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: Society for Neuroscience 2022
• [Presentation] Sleep/wake behavior of mice lacking PKA phosphorylation sites in SIK3 (2022)
  • Author(s): Minjeong Park, Takato Honda, Kanako Iwasaki, Chika Miyoshi, Tomoyuki Fujiyama, Miyo Kakizaki, Aya Ikkyu, Seiya Mizuno, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: Society for Neuroscience 2022
• [Presentation] Involvement of microglia in sleep/wake regulation in baseline conditions and under acute social defeat stress in mice (2022)
  • Author(s): Kazuya Miyanishi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: Society for Neuroscience 2022
• [Presentation] SIK3 kinase activity has a crucial role in sleep homeostatic regulation (2022)
  • Author(s): Shinya Nakata, Haruna Abe-Komiya, Tomoyuki Fujiyama, Chika Miyoshi, Aya Ikkyu, Fuyuki Asano, Kanako Iwasaki, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: Society for Neuroscience 2022
• [Presentation] Regulation of sleep amount by SIK3 in hypothalamic nuclei (2022)
  • Author(s): Kanako Iwasaki, Tomoyuki Fujiyama, Shinya Nakata, Minjeong Park, Chika Miyoshi, Noriko Hotta-Hirashima, Aya Ikkyu, Miyo Kakizaki, Yukiko Ishikawa, Fumihiro Sugiyama, Seiya Mizuno, Manabu Abe, Kenji Sakimura, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: Society for Neuroscience 2022
• [Presentation] 高感度超多重ISH法から観る内側視索前野のオス性行動における機能 (2022)
  • Author(s): 恒岡洋右、船戸弘正
  • Organizer: 第48回日本神経内分泌学会
• [Presentation] Neural ensembles activities underlying consolation-like behavior in mice (2022)
  • Author(s): Ryo Saito, Deependra Kumar, Hiromasa Funato, Masashi Yanagisawa, Aiba A.
  • Organizer: 日本神経学学会
• [Presentation] Molecular mechanisms for sleep/wake regulation mediated by SIK3 kinase activity (2022)
  • Author(s): Shinya Nakata, Haruna Abe-Komiya, Tomoyuki Fujiyama, Chika Miyoshi, Aya Ikkyu, Fuyuki Asano, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 日本神経学学会
• [Presentation] Involvement of microglia in sleep/wake regulation in baseline conditions and under acute social defeat stress in mice (2022)
  • Author(s): Kazuya Miyanishi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 日本神経学学会
• [Presentation] Sleep/wake behavior of mice lacking PKA phosphorylation site in SIK3 (2022)
  • Author(s): Minjeong Park, Takato Honda, Chika Miyoshi, Tomoyuki Fujiyama, Miyo Kakizaki, Aya Ikkyu, Seiya Mizuno, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 日本神経学学会
• [Presentation] Changes in sleep/wake by acute disruption in Hdac4 signaling (2022)
  • Author(s): Staci J. Kim, Shinya Nakata, Nodoka Aasama, Asmaa Elhosainy, Noriko Hotta-Hirashima, Aya Ikkyu, Miyo Kakizaki, Satomi Kanno, Tomoyuki Fujiyama, Seiya Mizuno, Satoru Takahashi, Chika Miyoshi, Hiromasa Funato, Masasyi Yanagisawa
  • Organizer: 日本神経学学会
• [Presentation] Neuroanatomical analysis of how the gain of function mutation in Sik3 increases sleep (2022)
  • Author(s): Kanako Iwasaki, Tomoyuki Fujiyama, Shinya Nakata, Minjeong Park, Chika Miyoshi, Noriko Hotta-Hirashima, Aya Ikkyu, Miyo Kakizaki, Yukiko Ishikawa, Fumihiro Sugiyama, Seiya Mizuno, Manabu Abe, Kenji Sakimura, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 日本神経学学会
• [Presentation] The role of Sleepy2 family in sleep/wake regulation in mice (2022)
  • Author(s): Takumi Tsukamoto, Staci J. Kim, Nodoka Asama, Fuyuki Asano, Tomoyuki Fujiyama, Aya Ikkyu, Noriko Hotta-Hirashima, Kumi Ebihara, Chika Miyoshi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 日本神経学学会
• [Presentation] ノンレム睡眠を制御する細胞内シグナル伝達系の解明 (2021)
  • Author(s): 船戸弘正
  • Organizer: 第46回睡眠学会
  • Invited
• [Presentation] Diversity of protein kinases that regulate sleep (2021)
  • Author(s): 船戸弘正
  • Organizer: 第44回神経科学学会
  • Invited
• [Presentation] Let the forward genetics guide us (2021)
  • Author(s): Hiromasa Funato
  • Organizer: 9th Annual IIIS symposium
  • Invited
• [Presentation] Physiological changes in parents and infants induced by physical contact (2020)
  • Author(s): Sachine Yoshida, Hiromasa Funato
  • Organizer: 第126回解剖学会第98回生理学会合同大会
• [Presentation] Intracellular signals in the regulation of sleep/wakefulness (2020)
  • Author(s): Hiromasa Funato
  • Organizer: 第126回解剖学会第98回生理学会合同大会
  • Invited
• [Presentation] Analysis of the molecular basis of sleep need through substrate screening for SIK3 (2020)
  • Author(s): Tomohiro Kitazono, Noriko-Hirashima Hotta, Kanako Iwasaki, Tomoyuki Fujiyama, Chika Miyoshi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第43回分子生物学会年会
• [Presentation] Sleep/wake behavior of mouse expressing a constitutive active form of SIK3 (2020)
  • Author(s): Shinya Nakata, Tomoyuki Fujiyama, Chika Miyoshi, Fuyuki Asano, Haruna Komiya, Aya Ikkyu, Mana Yamada, Juan Calros Neira Amlanza, Seiya Mizuno, Fumihiro Sugiyama, Satoru Takahashi, Hiromasa Funato, Masashi Yanagisawa
  • Organizer: 第43回分子生物学会年会
• [Presentation] オレキシン受容体作動薬による不眠症治療について (2020)
  • Author(s): 船戸弘正
  • Organizer: 日本病院薬剤師会関東ブロック第50回学術大会
  • Invited
• [Presentation] ショウジョウバエを用いた新規睡眠調節遺伝子HDAC4の機能解析 (2020)
  • Author(s): 羽鳥聖七, 小林里帆, 中川寛之, 冨田淳, 船戸弘正, 柳沢正史, 粂和彦
  • Organizer: Neuro2020
• [Presentation] 非選択的陽イオンチャネルNalcn遺伝子改変マウスを用いたレム睡眠制御機構の解析 (2020)
  • Author(s): 藤山知之, 管野里美, 堀田範子, 柿崎美代, 一久綾, 山田麻奈, 岩崎加奈子, 浅野冬樹, 望月文子, 水野聖哉, 阿部学, 三好千香, 上田壮志, 崎村建司, 高橋智, 船戸弘正, 柳沢正史
  • Organizer: Neuro2020
• [Presentation] SIK family regulates sleep and circadian behavior (2020)
  • Author(s): 船戸弘正
  • Organizer: Neuro2020
• [Presentation] Physiological responses in human infants during hugs by their parents (2020)
  • Author(s): 吉田さちね、川原圭博、笹谷拓也、清野健、小林洋、船戸弘正
  • Organizer: Neuro2020
• [Presentation] 若齢期に神経細胞特異的にSik3Sleepy変異を誘導したマウスは過眠を呈する (2020)
  • Author(s): 岩崎加奈子, 藤山知之, 中田慎也, パクミンジョン, 三好千香, 堀田範子, 一久綾, 柿﨑美代, 杉山文博, 水野聖哉, 阿部学, 﨑村建司, 高橋智, 船戸弘正, 柳沢正史
  • Organizer: Neuro2020
• [Presentation] マウスの睡眠制御におけるAP-2転写因子の役割の解析 (2020)
  • Author(s): 中井彩加、永田奈々恵、藤山知之、柏木光昭、一久綾、髙木眞莉奈、竜沢千加、田中可恵子、鹿糠実香、河野泰三、柿﨑美代、水野聖哉、杉山文博、高橋智、船戸弘正、柳沢正史、林悠
  • Organizer: Neuro2020
• [Presentation] Sleepy2 StoA変異マウスの作製 (2020)
  • Author(s): 浅間のどか, Staci J Kim, 藤山知之, 浅野冬樹, 三好千香, 堀田範子, 柿﨑美代, 一久綾, 管野里美, 中田慎也, 水野聖哉, 高橋智, 船戸弘正, 柳沢正史
  • Organizer: Neuro2020
• [Presentation] SIK3は睡眠要求と概日リズムを異なる神経細胞群を介して制御する (2020)
  • Author(s): 浅野冬樹, 藤山知之, 三好千香, 堀田範子, 柿崎美代, 一久綾, 管野里美, 中田慎也, 浅間のどか, 水野聖哉, 杉山文博, 高橋智, 船戸弘正, 柳沢正史
  • Organizer: Neuro2020
• [Presentation] Measurement of heart rate variability during a hug in parents and infants (2020)
  • Author(s): Sachine Yoshida, Yoshihiro Kawahara, Takuya Sasatani, Ken Kiyono, Yo Kobayashi, Hiromasa Funato
  • Organizer: International Congress of Infant Studies
  • Int'l Joint Research
• [Book] 睡眠学 (2020)
  • Author(s): 柳沢正史 船戸弘正
  • Total Pages: 712
  • Publisher: 朝倉書店
  • ISBN: 9784254301205
• [Book] ブレインサイエンスレビュー２０２ (2020)
  • Author(s): 船戸弘正
  • Total Pages: 346
  • Publisher: クバプロ
  • ISBN: 9784878051647
• [Patent(Industrial Property Rights)] 自家蛍光消光装置 (2021)
  • Inventor(s): 恒岡洋右 船戸弘正
  • Industrial Property Rights Holder: 恒岡洋右 船戸弘正
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2021-008882
  • Filing Date: 2021
• [Patent(Industrial Property Rights)] Hybridization chain reactionを安定に誘導する短鎖ヘアピンDNA配列とその精製法 (2020)
  • Inventor(s): 恒岡洋右 船戸弘正
  • Industrial Property Rights Holder: 恒岡洋右 船戸弘正
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2020-102648
  • Filing Date: 2020