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Strategy for dementia prevention based on the gut microbiota-brain axis on cognitive flexibility

Research Project

Project/Area Number 20H00575
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
Research InstitutionKyodo Milk Industry Co., Ltd.

Principal Investigator

Matsumoto Mitsuharu  協同乳業株式会社研究所, 研究所, 主幹研究員 (50505972)

Co-Investigator(Kenkyū-buntansha) 坪井 貴司  東京大学, 大学院総合文化研究科, 教授 (80415231)
掛山 正心  早稲田大学, 人間科学学術院, 教授 (30353535)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥45,370,000 (Direct Cost: ¥34,900,000、Indirect Cost: ¥10,470,000)
Fiscal Year 2022: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2021: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2020: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Keywords認知的柔軟性 / 腸内細菌叢 / 腸脳相関 / ホルモン / ポリアミン / 腸内細菌 / 脳腸相関 / 認知症 / GLP-1 / 腸内環境
Outline of Research at the Start

認知症の発症初期に低下する「認知的柔軟性」の維持に有用な食品に繋がる知見を、腸内細菌叢由来ポリアミン等の代謝産物と消化管ホルモンを介した脳腸軸(腸内細菌叢-ホルモン-脳システム)の分子基盤解明から得ることを目標とする。(1)マウス認知行動解析試験にて、認知的柔軟性に影響を与える腸内代謝産物とホルモンを決定する。(2)in vitro, in situ, in vivo試験、イメージング技術を用いて、それらのホルモン内分泌および脳神経系への作用を解析し、腸内細菌叢-ホルモン-脳システムの分子基盤を明らかにする。(3)それらの知見を認知症モデルマウスで検証し、食品による認知症予防戦略を提示する。

Outline of Final Research Achievements

This study aimed to calrify the relationship between intestinal microbiome and cognitive flexibility, which declines in the early stages of brain aging, through the intestinal microbiome-gut-brain axis.We developed a novel cognitive flexibility test protocol using touchscreen operant system. Using this protocol, we demonstrated that dysbiosis treated by streptomycin induces a decline in cognitive flexibility. We also found that treatment with a mixture of Bifidobacterium animalis subsp. lactis and arginine, which promote the production of intestinal bacterial polyamine, improves cognitive flexibility in mice. Concerning the intestinal microbiome-derived polyamine-related gut-brain axis mechanisms, we found that gut microbiome-derived polyamines are involved in the secretion of GLP-1 and cytokines, which appear to be important factors in the gut-brain axis, and the brain regions where the acquisition of cognitive flexibility causes changes in gene expression.

Academic Significance and Societal Importance of the Research Achievements

これまであまり研究対象とされて来なかった認知症発症前の可逆的ステージにおいて急激に低下する「認知的柔軟性」を標的とし、それをマウスで定量的に評価できる方法を構築した点は、この分野の今後の研究の発展に大きく貢献できるであろう。
また、認知的柔軟性に腸内細菌叢が関与することを世界に先駆け確認し、その低下を抑制できる食品候補(ビフィズス菌とアルギニンの併用投与)の提示に至った点は、超高齢社会に突入した我が国における認知症予防戦略上、高い意義を有する。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Comments on the Screening Results   Annual Research Report
  • Research Products

    (20 results)

All 2023 2022 2021 2020

All Journal Article (7 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] 腸内細菌叢由来ポリアミンが健康長寿の延伸に関与する2023

    • Author(s)
      松本光晴
    • Journal Title

      Microbiome Science

      Volume: 2 Pages: 37-43

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Bifidobacterium animalis subsp. lactis and arginine mixture intake improves cognitive flexibility in mice2023

    • Author(s)
      Kayo Ikuta, Daisuke Joho, Masaki Kakeyama, Mitsuharu Matsumoto
    • Journal Title

      Frontiers in Nutrition

      Volume: in press

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impact of intestinal microbiota on cognitive flexibility by a novel touch screen operant system task in mice2022

    • Author(s)
      Tamada H, Ikuta K, Makino Y, Joho D, Suzuki T, Kakeyama M, Matsumoto M
    • Journal Title

      Frontiers in Neuroscience

      Volume: 16 Pages: 882339-882339

    • DOI

      10.3389/fnins.2022.882339

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of red genetically encoded biosensor for visualization of intracellular glucose dynamics2022

    • Author(s)
      Mita Marie、Sugawara Izumi、Harada Kazuki、Ito Motoki、Takizawa Mai、Ishida Kentaro、Ueda Hiroshi、Kitaguchi Tetsuya、Tsuboi Takashi
    • Journal Title

      Cell Chemical Biology

      Volume: 29 Issue: 1 Pages: 98-108.e4

    • DOI

      10.1016/j.chembiol.2021.06.002

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells2022

    • Author(s)
      Osuga Yuri、Harada Kazuki、Tsuboi Takashi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 588 Pages: 118-124

    • DOI

      10.1016/j.bbrc.2021.12.043

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 腸内細菌叢と宿主の共益的なポリアミン代謝は大腸における上皮増殖とマクロファージ分化を調節する2021

    • Author(s)
      中村篤央,松本光晴,長谷耕二
    • Journal Title

      消化器病サイエンス

      Volume: 5 Pages: 233-236

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Symbiotic polyamine metabolism regulates epithelial proliferation and macrophage differentiation in the colon2021

    • Author(s)
      Nakamura Atsuo、Kurihara Shin、Takahashi Daisuke、Ohashi Wakana、Nakamura Yutaka、Kimura Shunsuke、Onuki Masayoshi、Kume Aiko、Sasazawa Yukiko、Furusawa Yukihiro、Obata Yuuki、Fukuda Shinji、Saiki Shinji、Matsumoto Mitsuharu、Hase Koji
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 2105-2105

    • DOI

      10.1038/s41467-021-22212-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 腸内細菌由来 Putrescine が腸管透過性に及ぼす影響2023

    • Author(s)
      中村篤央,生田かよ,久米愛子,栗原新,長谷耕二,松本光晴
    • Organizer
      日本ポリアミン学会第13回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 腸内ポリアミン産生誘導物質は菌叢撹乱マウスの認知的柔軟性の低下を改善する2023

    • Author(s)
      生田かよ,掛山正心,松本光晴
    • Organizer
      第77回 日本栄養・食糧学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 腸内マイクロバイオーム由来ポリアミンの生理機能2022

    • Author(s)
      松本光晴
    • Organizer
      第26回腸内細菌学会(シンポジウム)
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Effects of polyamines produced by intestinal bacteria on intestinal permeability.2022

    • Author(s)
      Nakamura A, Ikuta K, Kume A, Kurihara S, Hase K, Matsumoto M
    • Organizer
      International Human Microbiome Consortium 9th Congress 2022 KOBE
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウスの加齢に伴う認知的柔軟性の低下を捉えるタッチパネル装置定量評価系の確立2022

    • Author(s)
      生田かよ,松本光晴,掛山正心
    • Organizer
      第22回日本抗加齢医学会総会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 腸内細菌叢は生理活性物質ポリアミンの主要な供給源2022

    • Author(s)
      生田 かよ, 中村 篤央, 松本 光晴
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 健康寿命延伸を目指した腸内細菌叢の代謝産物を標的とする機能性食品開発2021

    • Author(s)
      松本光晴
    • Organizer
      Visionary 農芸化学100 シンポジウム 食・腸内細菌・健康研究領域 第3回シンポジウム
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Functions of polyamines produced by intestinal microbiome.2021

    • Author(s)
      Mitsuharu Matsumoto
    • Organizer
      The 11th Asian Conference for Lactic Acid Bacteria. Symposium 5. Microbial Metabolite and Cross-talk of Gut microbiota
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 腸内細菌叢と消化管内分泌細胞との機能連関2021

    • Author(s)
      坪井貴司
    • Organizer
      第74回日本自律神経学会総会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 腸内細菌叢と宿主の共役的なポリアミン代謝は大腸の上皮増殖とマクロファージ分化を調節する2021

    • Author(s)
      中村篤央,栗原新,松本光晴,長谷耕二
    • Organizer
      日本農芸化学会 2021年度大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 腸内ポリアミン産生誘導が若齢マウスの学習記憶に与える影響2020

    • Author(s)
      生田かよ,玉田葉月,松本光晴,掛山正心
    • Organizer
      第74回 日本栄養・食料学会大会
    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] ディスバイオーシスに伴う認知機能低下の症状を予防又は処置するための組成物および組合せ2023

    • Inventor(s)
      生田かよ, 松本光晴, 掛山正心
    • Industrial Property Rights Holder
      生田かよ, 松本光晴, 掛山正心
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2023-061138
    • Filing Date
      2023
    • Related Report
      2022 Annual Research Report
  • [Patent(Industrial Property Rights)] 中高齢の対象の認知的柔軟性を高めるための組成物および組合せ2022

    • Inventor(s)
      生田かよ, 松本光晴, 掛山正心
    • Industrial Property Rights Holder
      生田かよ, 松本光晴, 掛山正心
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2022-134149
    • Filing Date
      2022
    • Related Report
      2022 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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