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Fluid dynamical study of inertial focusing phenomena for application to cell separation

Research Project

Project/Area Number 20H02072
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 19010:Fluid engineering-related
Research InstitutionKansai University

Principal Investigator

Sugihara-Seki Masako  関西大学, システム理工学部, 教授 (80150225)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2023: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2022: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2021: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywords慣性集束現象 / 変形性 / 粘弾性 / Segre-Silberberg効果 / 粒子変形 / 慣性集中現象 / 細胞分離 / マイクロ流体デバイス / 流体 / 慣性
Outline of Research at the Start

最先端医療を含む広範な医学・生物の分野において,細胞集団から特定の細胞を選別する操作は不可欠となっている.流路形状や流動条件を適切に選ぶことにより,細胞の大きさや変形性の違いに応じて細胞の種類ごとに流路断面を流れる位置を制御することができるマイクロ流体デバイスは,細胞の分離・選別の目的のために極めて有望である.本研究では,細胞のように変形性を持つ粒子が粘弾性をもつ高分子水溶液に浮遊し,微小流路内を流れる場合に,流体から受ける揚力および流路断面における粒子分布を流体力学に基づき実験と数値解析によって調べ,粒子の分離・選別へ応用することを目指す.

Outline of Final Research Achievements

Particles suspended in a laminar flow through a narrow channel are known to migrate toward specific positions in the channel cross-section due to the presence of the inertial lift force. As the focusing position and focusing distance of particles depend on their size, shape and deformability, this phenomenon can be applied to the separation of suspended cells using suitably designed microfluidic devices. As a basis for the design of microfluidic devices, this study investigated the inertial migration of particles flowing through straight channels experimentally and numerically, focusing on the effect of deformability of suspended particles and the viscoelastic property of the suspending fluid.

Academic Significance and Societal Importance of the Research Achievements

細胞の混合液から特定の細胞を分離・検出する操作は,癌などの臨床診断を含む医学や生物学など広範囲な分野で不可欠となっている.現状は大掛かりな装置が必要であるのに対し,流れを利用した分離手法は小型で安価なマイクロ流体デバイスを用いるもので,細胞への負荷が小さいなど多くの利点を有することから,現在世界中で活発に研究が行われている.本研究では,有効なデバイス開発のための基礎研究として,細胞の特性である変形性や流体の粘弾性に着目して,流れ中の浮遊粒子の挙動を実験と数値解析により流体力学的観点から詳細に調べた.

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (54 results)

All 2023 2022 2021 2020 Other

All Journal Article (12 results) (of which Peer Reviewed: 11 results,  Open Access: 5 results) Presentation (38 results) (of which Int'l Joint Research: 14 results,  Invited: 7 results) Remarks (4 results)

  • [Journal Article] Margination of platelet-sized particles in red blood cell suspensions flowing through a Y-shaped confluence microchannel2023

    • Author(s)
      Noguchi Akira、Tange Yuki、Itano Tomoaki、Sugihara-Seki Masako
    • Journal Title

      Fluid Dynamics Research

      Volume: 55 Issue: 3 Pages: 035506-035506

    • DOI

      10.1088/1873-7005/acdf7c

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Numerical reproduction of the spiral wave visualized experimentally in a wide-gap spherical Couette flow2023

    • Author(s)
      Kazuki Yoshikawa; Tomoaki Itano; Masako Sugihara-Seki
    • Journal Title

      Physics of Fluids

      Volume: 35 Issue: 3 Pages: 034110-034110

    • DOI

      10.1063/5.0144365

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inertial Focusing of Red Blood Cells Suspended in Square Capillary Tube Flows2022

    • Author(s)
      Tanaka Saori、Sugihara-Seki Masako
    • Journal Title

      Journal of the Physical Society of Japan

      Volume: 91 Issue: 8 Pages: 083401-083401

    • DOI

      10.7566/jpsj.91.083401

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rheological characterization and flow reconstruction of polyvinylpyrrolidone aqueous solutions by means of velocity profiling-based rheometry2022

    • Author(s)
      Ohie Kohei、Yoshida Taiki、Tasaka Yuji、Sugihara-Seki Masako、Murai Yuichi
    • Journal Title

      Experiments in Fluids

      Volume: 63 Issue: 8 Pages: 135-135

    • DOI

      10.1007/s00348-022-03489-4

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inertial migration of red blood cells under a Newtonian fluid in a circular channel2022

    • Author(s)
      Takeishi Naoki、Yamashita Hiroshi、Omori Toshihiro、Yokoyama Naoto、Wada Shigeo、Sugihara-Seki Masako
    • Journal Title

      Journal of Fluid Mechanics

      Volume: 952 Pages: 952-952

    • DOI

      10.1017/jfm.2022.936

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Variation of focusing patterns of laterally migrating particles in a square-tube flow due to non-Newtonian elastic force2021

    • Author(s)
      Yokoyama Naoto、Yamashita Hiroshi、Higashi Kento、Miki Yuta、Itano Tomoaki、Sugihara-Seki Masako
    • Journal Title

      Physical Review Fluids

      Volume: 6 Issue: 7

    • DOI

      10.1103/physrevfluids.6.l072301

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Axial and Nonaxial Migration of Red Blood Cells in a Microtube2021

    • Author(s)
      Takeishi Naoki、Yamashita Hiroshi、Omori Toshihiro、Yokoyama Naoto、Sugihara-Seki Masako
    • Journal Title

      Micromachines

      Volume: 12 Issue: 10 Pages: 1162-1162

    • DOI

      10.3390/mi12101162

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Margination of Platelet-Sized Particles in the Red Blood Cell Suspension Flow through Square Microchannels2021

    • Author(s)
      Sugihara-Seki Masako、Takinouchi Nozomi
    • Journal Title

      Micromachines

      Volume: 12 Issue: 10 Pages: 1175-1175

    • DOI

      10.3390/mi12101175

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pattern Transition on Inertial Focusing of Neutrally Buoyant Particles Suspended in Rectangular Duct Flows2021

    • Author(s)
      Yamashita Hiroshi、Akinaga Takeshi、Sugihara-Seki Masako
    • Journal Title

      Micromachines

      Volume: 12 Issue: 10 Pages: 1242-1242

    • DOI

      10.3390/mi12101242

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 微小正方形管内血漿流れに浮遊する赤血球の断面内分布2021

    • Author(s)
      田中沙織、板野智昭、関眞佐子
    • Journal Title

      ながれ

      Volume: 40 Pages: 386-389

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 正方形管内流れのSEGRE-SILBERBERG 効果2021

    • Author(s)
      関眞佐子
    • Journal Title

      B&R

      Volume: 35 Pages: 69-74

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of margination of platelet-sized particles in red blood cell suspensions flowing through Y-shaped bifurcating microchannels2020

    • Author(s)
      Sugihara-Seki Masako, Onozawa Tenki, Takinouchi Nozomi, Itano Tomoaki, Seki Junji
    • Journal Title

      Biorheology

      Volume: 57 Issue: 2-4 Pages: 101-116

    • DOI

      10.3233/bir-201010

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] Effect of particle size on inertial particle focusing in square duct flows2023

    • Author(s)
      Hiroshi Yamashita, Takeshi Akinaga, Masako Sugihara-Seki
    • Organizer
      The 11th International Conference on Multiphase Flow
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Inertial focusing of red blood cells in square tube flows of blood plasma2023

    • Author(s)
      Masako Sugihara-Seki, Saori Tanaka
    • Organizer
      The 11th International Conference on Multiphase Flow
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 微細管を流れる血球の流体力学2023

    • Author(s)
      関眞佐子
    • Organizer
      第46回日本バイオレオロジー学会年会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 微小正方形管内流れ中のヒドロゲル粒子の断面内分布2023

    • Author(s)
      西山朋宏,佐井一総,湊遥香,鈴木大介,板野智昭,関眞佐子
    • Organizer
      第46回日本バイオレオロジー学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 正方形管内流れ中の扁平楕円体運動の解析2023

    • Author(s)
      藤澤勇亮,山下博士,板野智昭,関眞佐子
    • Organizer
      第46回日本バイオレオロジー学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Inertial focusing of spherical particles suspended in square tube flows of viscoelastic fluids2023

    • Author(s)
      Yoneyama, D., Miki, Y., Yamashita, H., Yokoyama, N., Itano, T. and Sugihara-Seki, M.
    • Organizer
      AJKFluids 2023 (ASME-JSME-KSME Fluid Engineering Division)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Numerical analysis of cellular flows spanning single red blood cell dynamics to bulk suspension rheology2023

    • Author(s)
      Takeishi, N., Rosti, M.E., Yokoyama, N., Sugihara-Seki, M. and Brant, L.
    • Organizer
      AJKFluids 2023 (ASME-JSME-KSME Fluid Engineering Division)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 管内層流に浮遊する粒子の慣性集束現象2023

    • Author(s)
      関眞佐子
    • Organizer
      混相流シンポジウム2023
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] ダクト内流れにおける超弾性体粒子の平衡位置に対する変形の影響2023

    • Author(s)
      廣畑佑真, 杉山和靖, 関眞佐子, 丹下祐希
    • Organizer
      混相流シンポジウム2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] 矩形管内層流に浮遊する剛体球粒子に働く力と粒子集束位置の数値解析2023

    • Author(s)
      政拓馬、山下博士、秋永剛、板野智昭、関眞佐子
    • Organizer
      日本流体力学会年会2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] 正方形管流れに浮遊する超弾性体粒子の慣性集束現象2023

    • Author(s)
      丹下祐希、関眞佐子、杉山和靖、廣畑佑真
    • Organizer
      日本流体力学会年会2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] 矩形管ながれによる慣性粒子の分離2023

    • Author(s)
      秋永剛、山下博士、関眞佐子
    • Organizer
      日本流体力学会年会2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] Margination of platelet-sized particles in red blood cell suspensions flowing through a Y-shaped microchannel2023

    • Author(s)
      Sugihara-Seki, M.
    • Organizer
      3rd World Congress of ESCHM-ISCH-ISB
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of medium elasticity on the inertial fucusing of spherical particles in square tube flows2023

    • Author(s)
      Yoneyama, D., Miki, Y., Yamashita, H., Yokoyama, N., Tagawa, Y., Itano, T., Sugihara-Seki, M.
    • Organizer
      76th Annual Meeting of the Americal Physical Society, Division of Fluid Dynamics
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development lengths for the margination of platelet-sized particles after the confluence and bifurcation of Y-shaped microchannels2023

    • Author(s)
      Nagaishi, K., Noguchi, A., Onozawa, T., Itano, T., Sugihara-Seki, M.
    • Organizer
      76th Annual Meeting of the Americal Physical Society, Division of Fluid Dynamics
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ニュートン流体と粘弾性流体中の慣性集束現象2023

    • Author(s)
      関眞佐子
    • Organizer
      第71回レオロジー討論会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 微小正方形管内流れに浮遊する赤血球の管断面内分布2022

    • Author(s)
      関 眞佐子, 田中沙織, 佐井一総, 板野智昭
    • Organizer
      第45回日本バイオレオロジー学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 慣性効果による赤血球の円管路断面内の移動に関する数値解析2022

    • Author(s)
      武石直樹,山下博士,横山直人,関 眞佐子
    • Organizer
      第66回理論応用力学講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Axial and nonaxial migration of red blood cells under a Newtonian fluid in microchannels2022

    • Author(s)
      Takeishi, N., Yamashita, H., Omori, T., Yokoyama, N., Sugihara-Seki, M.
    • Organizer
      9th World Congress of Biomechanics
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 山下博士,秋永剛,関眞佐子:正方形管内サスペンション流れで見られる粒子集中現象とそのパターン変化2022

    • Author(s)
      山下博士,秋永剛,関眞佐子
    • Organizer
      日本流体力学会中四国・九州支部第29 回講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 微小矩形管内層流に浮遊するゲル粒子の断面内分布2022

    • Author(s)
      佐井一総,湊遥香,鈴木大介,板野智昭,関眞佐子
    • Organizer
      日本流体力学会年会2022
    • Related Report
      2022 Annual Research Report
  • [Presentation] 粘弾性流体の正方形管内流れに浮遊する球形粒子の管断面内分布2022

    • Author(s)
      米山大輔,三木祐太,山下博士,横山直人,田川義之,板野智昭,関眞佐子
    • Organizer
      日本流体力学会年会2022
    • Related Report
      2022 Annual Research Report
  • [Presentation] 正方形管内粘弾性流体流れ中の粒子に作用する揚力の数値解析2022

    • Author(s)
      東健人,山下博士,横山直人,板野智昭,関眞佐子
    • Organizer
      日本流体力学会年会2022
    • Related Report
      2022 Annual Research Report
  • [Presentation] 円管路内の赤血球の慣性移動に関する数値解析2022

    • Author(s)
      武石直樹,山下博士,大森俊宏,横山直人,和田成生,関眞佐子
    • Organizer
      日本流体力学会年会2022
    • Related Report
      2022 Annual Research Report
  • [Presentation] 赤血球サスペンションの合流流れにおける血小板模擬粒子の分布2022

    • Author(s)
      野口慧,長石弘大,板野智昭,関眞佐子
    • Organizer
      日本流体力学会年会2022
    • Related Report
      2022 Annual Research Report
  • [Presentation] 微小長方形流路内赤血球流れ中の血小板模擬粒子の断面内分布の進展と流路幅の影響2022

    • Author(s)
      長石弘大,野口慧,板野智昭,関眞佐子
    • Organizer
      日本流体力学会年会2022
    • Related Report
      2022 Annual Research Report
  • [Presentation] 微小血管内の血液流れと血球運動2021

    • Author(s)
      関眞佐子
    • Organizer
      京都大学数理解析研究所 Tutorial Seminar 「生物の創るパターンとダイナミクス:基礎からの展開」
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Lateral migration of red blood cells in capillary tube flows2021

    • Author(s)
      Sugihara-Seki Masako
    • Organizer
      Biofluid Symposium (RIMS Research Project Biofluids 2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Inertial focusing of red blood cells suspended in blood plasma flowing through square tubes2021

    • Author(s)
      Sugihara-Seki Masako, Tanaka Saori
    • Organizer
      2nd Joint Meeting of ESCHM-ISCH-ISB
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Inertial focusing of spherical particles suspended in square channel flows2021

    • Author(s)
      Sugihara-Seki Masako, Yamashita Hiroshi
    • Organizer
      25th International Congress of Theoretical and Applied Mechanics
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Inertial focusing of rigid particles and red blood cells in straight square tube flows2021

    • Author(s)
      Sugihara-Seki Masako
    • Organizer
      Inertial Microfluidics: Challenges and Perspectives
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Comprehensive evaluation for viscoelasticity of polyvinylpyrrolidone aqueous solutions by utilizing novel map representation based on ultrasonic spinning rheometry2021

    • Author(s)
      Ohie Kohei, Yoshida Taiki, Tasaka Yuji, Sugihara-Seki Masako, Murai Yuichi
    • Organizer
      13th InternationalSymposium on Ultrasonic Doppler Methods for Fluid Mechanics and Fluid Engineering
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 微小正方形管内流れにおける赤血球の断面内分布-硬化度の影響-2020

    • Author(s)
      田中沙織,板野智昭,関眞佐子
    • Organizer
      日本バイオレオロジー学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 長方形管内サスペンション流れで見られるTubular Pinch 効果2020

    • Author(s)
      山下博士,関眞佐子
    • Organizer
      日本流体力学会年会2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] 微小正方形管内の粘弾性流体流れに浮遊する球形粒子の断面内分布2020

    • Author(s)
      三木祐太,山下博士,横山直人,板野智昭,関 眞佐子
    • Organizer
      日本流体力学会年会2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] 矩形管内粘弾性流体流れ中の粒子に作用する揚力の数値解析2020

    • Author(s)
      東健人, 山下博士, 横山直人,板野智昭, 関眞佐子
    • Organizer
      日本流体力学会年会2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] 微小正方形管流れに浮遊する赤血球の断面内分布-硬化度依存性-2020

    • Author(s)
      田中沙織,板野智昭,関眞佐子
    • Organizer
      日本流体力学会年会2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] Inertial focusing patterns and their transition for a neutrally buoyant sphere suspended in rectangular duct flow2020

    • Author(s)
      Yamashita Hiroshi & Sugihara-Seki Masako
    • Organizer
      73rd Annual Meeting of the APS Division of Fluid Dynamics
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 関西大学学術情報システム

    • URL

      https://gakujo.kansai-u.ac.jp/

    • Related Report
      2023 Annual Research Report
  • [Remarks] 学術情報システム

    • URL

      https://gakujo.kansai-u.ac.jp/search/index.jsp

    • Related Report
      2022 Annual Research Report
  • [Remarks] 流体物理研究室ホームページ

    • URL

      http://fluid.phys.kansai-u.ac.jp/

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
  • [Remarks] 関西大学学術リポジトリ

    • URL

      https://kansai-u.repo.nii.ac.jp/?page_id=13

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2025-01-30  

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