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Development of nitrogen removal technology in a single sewage treatment plant aeration tank with accelerated aerobic denitrification reaction

Research Project

Project/Area Number 20H02290
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 22060:Environmental systems for civil engineering-related
Research InstitutionIchinoseki National College of Technology (2021-2023)
Nagaoka National College of Technology (2020)

Principal Investigator

Araki Nobuo  一関工業高等専門学校, その他部局等, 校長 (30193072)

Co-Investigator(Kenkyū-buntansha) 川上 周司  長岡工業高等専門学校, 環境都市工学科, 准教授 (00610461)
青木 仁孝  国立研究開発法人国立環境研究所, 地域環境保全領域, 研究員 (80775809)
押木 守  北海道大学, 工学研究院, 准教授 (90540865)
渡利 高大  長岡技術科学大学, 工学研究科, 助教 (90800540)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2023: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2022: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2021: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywords好気性脱窒細菌 / 従属栄養硝化脱窒反応 / 都市下水処理 / 窒素除去 / 脱窒素反応 / 好気性脱窒素細菌 / 好気性脱窒素反応 / 好気性脱窒素 / 好気性脱窒反応 / 全窒素除去 / エアレーションタンク / 脱窒素細菌群
Outline of Research at the Start

都市下水の処理水の放流は自然水系の富栄養化現象の一因となっている。閉鎖性水域を抱える一部地域ではこれを防止するために嫌気好気法などの高度処理が適用されている。しかし、通常の活性汚泥法でも運転条件によっては高度処理と同等の窒素除去性能を示している場合もある。本研究は好気性脱窒細菌の定着と能力を最大限に引き出す運転条件を明らかにし、標準活性汚泥法のエアレーションタンク単槽でも現在の高度処理と同等の窒素除去能力を達成する技術を開発する。

Outline of Final Research Achievements

In this study, the effect of DO concentration on aerobic denitrification bacteria was investigated. Since the DO concentration cannot be controlled by the shaking culture method reported previously, an apparatus to maintain a constant dissolved oxygen concentration was constructed in this study and aerobic denitrification tests were conducted under different DO concentration conditions. Aerobic denitrification proceeded simultaneously with nitrification under all aerobic conditions. The results of the bacterial flora analysis based on rRNA genes showed that bacteria of the genera Paracoccus and Pseudomonas predominated as the DO concentration increased, revealing a competitive relationship in a complex microbial system. A tracer test using stable isotope 15N-labelled nitric acid on the sludge confirmed the formation of N2, confirming an aerobic denitrification reaction.

Academic Significance and Societal Importance of the Research Achievements

これまで報告されていた好気性脱窒反応の多くは振盪培養法によってDOを制御しており、細菌群が対数増殖期に入ると十分なDOを確保できず、嫌気的な脱窒反応も混在していたことが予想され、多くの研究者の疑念を払拭できずにいた。本研究では、DOを維持する装置を作成し、5mg/Lといった高いDO条件下でも好気性脱窒反応を確認した。またDO濃度の違いにより優占する細菌群が異なることも示した。このことは実現場で起きている好気性脱窒のメカニズム解明を後押しする重要な知見であり、今後好気性脱窒反応を卓越させた脱窒処理技術の開発に大きく寄与するものと思われる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (27 results)

All 2024 2023 2022 2021

All Journal Article (18 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 18 results,  Open Access: 13 results) Presentation (9 results)

  • [Journal Article] Collaborative metabolisms of urea and cyanate degradation in marine anammox bacterial culture2024

    • Author(s)
      Oshiki Mamoru、Morimoto Emi、Kobayashi Kanae、Satoh Hisashi、Okabe Satoshi
    • Journal Title

      ISME Communications

      Volume: 4 Issue: 1 Pages: 1-14

    • DOI

      10.1093/ismeco/ycad007

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] EFFECTS OF DIFFERENT DISSOLVED OXYGEN CONDITIONS ON AEROBIC DENITRIFYING BACTERIA IN A COMPLEX MICROBIAL COMMUNITY2023

    • Author(s)
      上村 光輝, 川上 周司, 押木 守, 青木 仁孝, 土田 勝範, 渡利 高大, 荒木 信夫
    • Journal Title

      Japanese Journal of JSCE

      Volume: 79 Issue: 25 Pages: n/a

    • DOI

      10.2208/jscejj.23-25034

    • ISSN
      2436-6021
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Denitrification in low oxic environments increases the accumulation of nitrogen oxide intermediates and modulates the evolutionary potential of microbial populations2023

    • Author(s)
      Takahashi Kohei、Oshiki Mamoru、Ruan Chujin、Morinaga Kana、Toyofuku Masanori、Nomura Nobuhiko、Johnson David R.
    • Journal Title

      Environmental Microbiology Reports

      Volume: 16 Issue: 1 Pages: 0-0

    • DOI

      10.1111/1758-2229.13221

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Enhanced sulfide removal by gas stripping in a novel reactor for anaerobic wastewater treatment2023

    • Author(s)
      Onodera Takashi、Takemura Yasuyuki、Aoki Masataka、Syutsubo Kazuaki
    • Journal Title

      Water Science & Technology

      Volume: 87 Issue: 9 Pages: 2223-2232

    • DOI

      10.2166/wst.2023.120

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and physiological properties of methanogenic archaea that degrade tetramethylammonium hydroxide2023

    • Author(s)
      Iguchi Akinori、Takemura Yasuyuki、Danshita Tsuyoshi、Kurihara Takuya、Aoki Masataka、Hori Saori、Shigematsu Toru、Syutsubo Kazuaki
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 107 Issue: 9 Pages: 3047-3056

    • DOI

      10.1007/s00253-023-12488-2

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative detection and reduction of potentially pathogenic bacterial groups of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex, and Mycobacterium in wastewater treatment facilities2023

    • Author(s)
      Aoki Masataka、Takemura Yasuyuki、Kawakami Shuji、Yoochatchaval Wilasinee、Tran P. Thao、Tomioka Noriko、Ebie Yoshitaka、Syutsubo Kazuaki
    • Journal Title

      PLOS ONE

      Volume: 18 Issue: 9 Pages: e0291742-e0291742

    • DOI

      10.1371/journal.pone.0291742

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and cultivation of anaerobic bacterial scavengers of dead cells2023

    • Author(s)
      Hirakata Yuga、Mei Ran、Morinaga Kana、Katayama Taiki、Tamaki Hideyuki、Meng Xian-ying、Watari Takahiro、Yamaguchi Takashi、Hatamoto Masashi、Nobu Masaru K
    • Journal Title

      The ISME Journal

      Volume: 17 Issue: 12 Pages: 2279-2289

    • DOI

      10.1038/s41396-023-01538-2

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sewage-derived substances and dead microbial cells cause gel biofilm formation in membrane bioreactors treating real sewage2023

    • Author(s)
      Toru Miwa, Yuya Takimoto, Yujiro Tokuda, Takahiro Watari, Takashi Yamaguchi, Masashi Hatamoto
    • Journal Title

      Chemical Engineering Journal

      Volume: 472 Pages: 144882-144882

    • DOI

      10.1016/j.cej.2023.144882

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Growth of nitrite‐oxidizing Nitrospira and ammonia‐oxidizing Nitrosomonas in marine recirculating trickling biofilter reactors2022

    • Author(s)
      Oshiki Mamoru、Netsu Hirotoshi、Kuroda Kyohei、Narihiro Takashi、Fujii Naoki、Kindaichi Tomonori、Suzuki Yoshiyuki、Watari Takahiro、Hatamoto Masashi、Yamaguchi Takashi、Araki Nobuo、Okabe Satoshi
    • Journal Title

      Environmental Microbiology

      Volume: 24 Issue: 8 Pages: 3735-3750

    • DOI

      10.1111/1462-2920.16085

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] NH<sub>2</sub>OH Disproportionation Mediated by Anaerobic Ammonium-oxidizing (Anammox) Bacteria2022

    • Author(s)
      Oshiki Mamoru、Gao Lin、Zhang Lei、Okabe Satoshi
    • Journal Title

      Microbes and Environments

      Volume: 37 Issue: 2 Pages: n/a

    • DOI

      10.1264/jsme2.ME21092

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] N<sub>2</sub>O Reduction by <i>Gemmatimonas aurantiaca</i> and Potential Involvement of <i>Gemmatimonadetes</i> Bacteria in N<sub>2</sub>O Reduction in Agricultural Soils2022

    • Author(s)
      Mamoru Oshiki, Yuka Toyama, Toshikazu Suenaga, Akihiko Terada, Yasuhiro Kasahara, Takashi Yamaguchi, Nobuo Araki
    • Journal Title

      Microbes and Environments

      Volume: 37 Issue: 2 Pages: n/a

    • DOI

      10.1264/jsme2.ME21090

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of denitrifying granular sludge addition on activated sludge and anaerobic-aerobic systems for municipal sewage treatment2022

    • Author(s)
      Takahiro Watari, Kenya Asano, Takanori Omine, Masashi Hatamoto, Nobuo Araki, Kazuhisa Mimura, Akihiro Nagano & Takashi Yamaguchi
    • Journal Title

      Journal of Environmental Science and Health, Part A

      Volume: 57(9) Issue: 9 Pages: 830-839

    • DOI

      10.1080/10934529.2022.2118485

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Stable denitrification performance of a mesh rotating biological reactor treating municipal wastewate2022

    • Author(s)
      Shehani Sharadha Maheepala, Shunji Fuchigami, Masashi Hatamoto, Takumi Akashi, Takahiro Watari, Takashi Yamaguchi
    • Journal Title

      Environmental Technology and Innovation

      Volume: 25 Pages: 102543-102543

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of inoculum sources on autotrophic nitrogen removal in anaerobic hollow fiber membrane reactors2022

    • Author(s)
      Thao Tran P, Masashi Hatamoto, Masataka Aoki, Takahiro Watari, Kazuaki Syutsubo, Takashi Yamaguchi
    • Journal Title

      Environmental Technology and Innovation

      Volume: 25 Pages: 102375-102375

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Determination of 15N-14N of Ammonium, Nitrite, Nitrate, Hydroxylamine, and Hydrazine Using Colorimetric Reagents and Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS)2022

    • Author(s)
      M.Oshiki, K.Nagai, S.Ishii,Y.Suzuki, T.Yamaguchi, N.Araki, S.Okabe
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 88

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a single-stage mainstream anammox process using a sponge-bed trickling filter2021

    • Author(s)
      Takahiro Watari, Carlos Lopez Vazquez, Masashi Hatamoto, Takashi Yamaguchi, Jules B van Lier
    • Journal Title

      Environmental Technology

      Volume: 42 Pages: 3036-3047

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of salinities on nitrogen removal performance of DHS-USB system and growth of Epinephelus bruneus in closed recirculating aquaculture system2021

    • Author(s)
      Wilasinee Kotcharoen, Takahiro Watari, Nur Adlin, Yoshinobu Nakamura, Penpicha Satanwat, Wiboonluk Pungrasmi, Sorawit Powtongsook, Yutaka Takeuchi, Masashi Hatamoto, Shinichi Yamazaki, Takashi Yamaguchi
    • Journal Title

      International Biodeterioration and Biodegradation

      Volume: 164 Pages: 105299-105299

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long-term treatment of municipal wastewater using a mesh rotating biological reactor and changes in the biofilm community2021

    • Author(s)
      Shunji Fuchigami, Masashi Hatamoto, Ryota Takagi, Takumi Akashi, Takahiro Watari, Takashi Yamaguchi
    • Journal Title

      Environmental Technology and Innovation

      Volume: 24 Pages: 102074-102074

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 活性汚泥に生息する従属栄養硝化-好気性脱窒細菌の特定2024

    • Author(s)
      上村光輝 , 山本和佳奈 , 川上周司 , 渡利高大 , 押木守 , 青木仁孝 , 荒木信夫
    • Organizer
      第58回日本水環境学会年会講演集
    • Related Report
      2023 Annual Research Report
  • [Presentation] 多重染色 FISH 法を用いた Comammox 細菌の視覚的検出2024

    • Author(s)
      山田灯乃助 , 川上周司 , 渡利高大 , 松浦哲久
    • Organizer
      第58回日本水環境学会年会講演集
    • Related Report
      2023 Annual Research Report
  • [Presentation] 光エネルギーで駆動する生物光化学的NH4+再生システムの開発2024

    • Author(s)
      進藤 琉司 , 押木 守 , 岡部 聡
    • Organizer
      第58回日本水環境学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Anammox細菌による亜硝酸酸化反応の窒素バックフラックス2024

    • Author(s)
      河崎 聖也 , 押木 守 , 岡部 聡
    • Organizer
      第58回日本水環境学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Potential evaluation of autotrophic denitrification in sediment bio-electrochemical systems2023

    • Author(s)
      Thao Tran P , Yasuyuki Takemura , Masataka Aoki , Noriko Tomioka , Kazuaki Syutsubo
    • Organizer
      2023 Asia-Pacific International Society of Microbial Electrochemistry and Technology (AP-ISMET) Conference
    • Related Report
      2023 Annual Research Report
  • [Presentation] DHSリアクターによるComammox細菌の集積培養の試み2023

    • Author(s)
      佐藤友紀 , 渡利高大 , 幡本将史 , 山口隆司
    • Organizer
      第58回日本水環境学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 嫌気共生細菌を優占的に保持可能な微生物担体における含有導電性材料が微生物群集に与える影響2023

    • Author(s)
      根津拓福 , 幡本将史 , 渡利高大 , 山口隆司
    • Organizer
      第58回日本水環境学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 都市下水処理を対象とした活性汚泥に生息する好気性脱窒細菌の特定2023

    • Author(s)
      上村光輝, 川上周司, 渡利高大, 青木仁孝, 押木守, 荒木信夫
    • Organizer
      第57回日本水環境学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] DNA aptamer を用いた DNA 抽出不要の微生物定量技術の開発2023

    • Author(s)
      小林勇貴、関口未来莉、川上周司、渡利高大、山口隆司、幡本将史
    • Organizer
      第57回日本水環境学会年会
    • Related Report
      2022 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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