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Development of innovative prodrug design for cancer therapeutic bispecific antibodies

Research Project

Project/Area Number 20H02534
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Asano Ryutaro  東京農工大学, 工学(系)研究科(研究院), 教授 (80323103)

Co-Investigator(Kenkyū-buntansha) 稲田 全規  東京農工大学, 工学(系)研究科(研究院), 准教授 (80401454)
安永 正浩  国立研究開発法人国立がん研究センター, 先端医療開発センター, 分野長 (80450576)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2020: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords二重特異性抗体 / プロドラッグ / プロテアーゼ
Outline of Research at the Start

がん細胞と免疫細胞間の強制的な架橋により強力な抗腫瘍効果を発揮する二重特異性抗体は、次世代型の人工抗体医薬として期待されている。しかし、これまでに高分子量型と低分子量型の二重特異性抗体が一つずつ認可されているが、いずれも一長一短がある。また、がん特異的な抗原は見出されていないため、免疫細胞による正常組織の障害が共通の問題となっている。本研究では、これらを解消するために、がん特異的プロテアーゼの認識配列を二重特異性抗体に巧みに組み込むことで、高分子量型と低分子量型の両長所のみを併せ持ち、かつ免疫細胞に対して低親和性型から高親和性型に腫瘍送達後に構造変換するプロドラッグ化デザインの開発を目指す。

Outline of Final Research Achievements

We developed novel bispecific antibody prodrug design that improves its function after delivery to cancer tissue. As a result of fusion of the amino acid polymer sequences to bispecific antibodies via the cancer-related protease recognition site, inhibition of both binding ability and cytotoxic activity was observed dependent on the length of the amino acid polymer. In contrast, both binding ability and cytotoxic activity were restored completely by the removal of the amino acid polymers using the cancer-related protease.

Academic Significance and Societal Importance of the Research Achievements

二重特異性抗体をはじめ次世代抗体は強い薬効が期待される一方で副作用も懸念されている。がん組織送達後に結合能が向上するような二重特異性抗体をデザインするために結合阻害配列の検討を行った結果、アミノ酸ポリマー配列を用いることが、汎用的、かつ重合度に応じて結合阻害度を制御できることが明らかになった。一方、このアミノ酸ポリマーを除去することで結合活性の完全な回復が確認されたため、今後次世代抗体の副作用の低減に向けた汎用的な技術となることが期待される。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (65 results)

All 2023 2022 2021 2020

All Journal Article (26 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 24 results,  Open Access: 22 results) Presentation (39 results) (of which Int'l Joint Research: 3 results,  Invited: 6 results)

  • [Journal Article] Prostate cancer expressing membrane-bound TGF-α induces bone formation mediated by the autocrine effect of prostaglandin E2in osteoblasts.2023

    • Author(s)
      Yoshinouchi S, Karouji K, Tominari T, Sugasaki M, Matsumoto C, Miyaura C, Hirata M, Itoh Y, Inada M.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 644 Pages: 40-48

    • DOI

      10.1016/j.bbrc.2022.11.050

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] RNA-binding protein LIN28A upregulates transcription factor HIF1α by posttranscriptional regulation via direct binding to UGAU motifs2023

    • Author(s)
      Yamamoto Hiroto、Uchida Yutaro、Kurimoto Ryota、Chiba Tomoki、Matsushima Takahide、Ito Yoshiaki、Inotsume Maiko、Miyata Kohei、Watanabe Kenta、Inada Masaki、Goshima Naoki、Uchida Tokujiro、Asahara Hiroshi
    • Journal Title

      Journal of Biological Chemistry

      Volume: 299 Issue: 1 Pages: 102791-102791

    • DOI

      10.1016/j.jbc.2022.102791

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Universal Design of Luciferase Fusion Proteins for Epigenetic Modifications Detection Based on Bioluminescence Resonance Energy Transfer2023

    • Author(s)
      Miyata T., Shimamura H., Asano R., Yoshida W.
    • Journal Title

      Anal. Chem.

      Volume: 95 Issue: 7 Pages: 3799-3805

    • DOI

      10.1021/acs.analchem.2c05066

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a Versatile Method to Construct Direct Electron Transfer-Type Enzyme Complexes Employing SpyCatcher/SpyTag System2023

    • Author(s)
      Yanase T., Okuda-Shimazaki J., Asano R., Ikebukuro K., Sode K., Tsugawa W.
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 24 Issue: 3 Pages: 1837-1837

    • DOI

      10.3390/ijms24031837

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of β-cryptoxanthin on bone metabolisms2022

    • Author(s)
      Tominari T, Matsumoto C, Karouji K,Tanaka Y,Takatoya M,Inada M, Hirata M
    • Journal Title

      Functional Food Research

      Volume: 18 Issue: 0 Pages: 28-33

    • DOI

      10.32153/ffr.ffr18_p28-33

    • ISSN
      2432-3357, 2434-3048
    • Year and Date
      2022-09-12
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Caveolin-3 regulates the activity of Ca<sup>2+</sup>/calmodulin-dependent protein kinase II in C2C12 cells2022

    • Author(s)
      Matsunobe Michio、Motohashi Norio、Aoki Eito、Tominari Tsukasa、Inada Masaki、Aoki Yoshitsugu
    • Journal Title

      American Journal of Physiology-Cell Physiology

      Volume: 323 Issue: 4 Pages: C1137-C1148

    • DOI

      10.1152/ajpcell.00077.2022

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structure of lactate oxidase from Enterococcus hirae revealed new aspects of active site loop function: Product-inhibition mechanism and oxygen gatekeeper2022

    • Author(s)
      Hiraka K., Yoshida H., Tsugawa W., Asano R., LaBelle J., Ikebukuro K., Sode K.
    • Journal Title

      Protein Sci.

      Volume: 31 Issue: 10

    • DOI

      10.1002/pro.4434

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Strategies to simplify operation procedures for applying labeled antibody-based immunosensors to point-of-care testing2022

    • Author(s)
      Kimura H., Asano R.
    • Journal Title

      Anal. Biochem.

      Volume: 654 Pages: 114806-114806

    • DOI

      10.1016/j.ab.2022.114806

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication of Fragment Antibody-Enzyme Complex as a Sensing Element for Immunosensing2022

    • Author(s)
      Oda M., Asano R
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 23 (3) Issue: 3 Pages: 1335-1335

    • DOI

      10.3390/ijms23031335

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 低分子型二重特異性抗体の高機能化戦略2022

    • Author(s)
      浅野竜太郎, 熊谷 泉
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 80 (1) Pages: 40-41

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Endosomal TLR3 signaling in stromal osteoblasts induces prostaglandin E2?mediated inflammatory periodontal bone resorption2022

    • Author(s)
      Tominari Tsukasa、Akita Miyuki、Matsumoto Chiho、Hirata Michiko、Yoshinouchi Shosei、Tanaka Yuki、Karouji Kento、Itoh Yoshifumi、Maruyama Takayuki、Miyaura Chisato、Numabe Yukihiro、Inada Masaki
    • Journal Title

      Journal of Biological Chemistry

      Volume: 298 Issue: 3 Pages: 101603-101603

    • DOI

      10.1016/j.jbc.2022.101603

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of Functional Food for Citrus and Application of Analysis for Mass Spectrometry Imaging2021

    • Author(s)
      芳之内 翔成、富成 司、平田 美智子、宮浦 千里、新間 秀一、稲田 全規
    • Journal Title

      Functional Food Research

      Volume: 17 Issue: 0 Pages: 63-68

    • DOI

      10.32153/ffr.ffr17_p63-68

    • NAID

      130008145182

    • ISSN
      2432-3357, 2434-3048
    • Year and Date
      2021-09-12
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rapid, convenient, and highly sensitive detection of human hemoglobin in serum using a high-affinity bivalent antibody-enzyme complex2021

    • Author(s)
      Miura D., Kimura H., Tsugawa W., Ikebukuro K., Sode K., Asano R.
    • Journal Title

      Talanta

      Volume: 234 Pages: 122638-122638

    • DOI

      10.1016/j.talanta.2021.122638

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of intercellular cross-linking abilities correlated with cytotoxicities of bispecific antibodies with domain rearrangements using AFM force-sensing2021

    • Author(s)
      Maejima A., Ishibashi K., Kim H., Kumagai I., Asano R.
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 178 Pages: 113037-113037

    • DOI

      10.1016/j.bios.2021.113037

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] T Cell Bispecific Antibodies: An Antibody-Based Delivery System for Inducing Antitumor Immunity2021

    • Author(s)
      Kamakura D., Asano R., Yasunaga M.
    • Journal Title

      Pharmaceuticals

      Volume: 14 (11) Issue: 11 Pages: 1172-1172

    • DOI

      10.3390/ph14111172

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 二重特異性がん治療抗体の機能的な構造の理解に向けたあゆみ2021

    • Author(s)
      浅野竜太郎, 真壁幸樹, 田中良和, 熊谷 泉
    • Journal Title

      医学のあゆみ

      Volume: 278 (6) Pages: 617-622

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Gram-positive bacteria cell wall-derived lipoteichoic acid induces inflammatory alveolar bone loss through prostaglandin E production in osteoblasts.2021

    • Author(s)
      Tominari T, Sanada A, Ichimaru R, Matsumoto C, Hirata M, Itoh Y, Numabe Y, Miyaura C, Inada M
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 13353-13353

    • DOI

      10.1038/s41598-021-92744-5

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A common SNP risk variant MT1-MMP causative for Dupuytren's disease has a specific defect in collagenolytic activity2021

    • Author(s)
      Itoh Yoshifumi、Ng Michael、Wiberg Akira、Inoue Katsuaki、Hirata Narumi、Paiva Katiucia Batista Silva、Ito Noriko、Dzobo Kim、Sato Nanami、Gifford Valentina、Fujita Yasuyuki、Inada Masaki、Furniss Dominic
    • Journal Title

      Matrix Biology

      Volume: 97 Pages: 20-39

    • DOI

      10.1016/j.matbio.2021.02.003

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Anti-EGFR antibody 528 binds to domain III of EGFR at a site shifted from the cetuximab epitope2021

    • Author(s)
      Makabe Koki、Yokoyama Takeshi、Uehara Shiro、Uchikubo-Kamo Tomomi、Shirouzu Mikako、Kimura Kouki、Tsumoto Kouhei、Asano Ryutaro、Tanaka Yoshikazu、Kumagai Izumi
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 5790-5790

    • DOI

      10.1038/s41598-021-84171-3

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rapid and homogeneous electrochemical detection by fabricating a high affinity bispecific antibody-enzyme complex using two Catcher/Tag systems2021

    • Author(s)
      Kimura Hayato、Miura Daimei、Tsugawa Wakako、Ikebukuro Kazunori、Sode Koji、Asano Ryutaro
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 175 Pages: 112885-112885

    • DOI

      10.1016/j.bios.2020.112885

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mechanism of action of a T cell-dependent bispecific antibody as a breakthrough immunotherapy against refractory colorectal cancer with an oncogenic mutation2021

    • Author(s)
      Kamakura D., Asano R., Kawai H., Yasunaga M.
    • Journal Title

      Cancer Immunology, Immunotherapy

      Volume: 70 Issue: 1 Pages: 177-188

    • DOI

      10.1007/s00262-020-02667-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Association behavior and control of the quality of cancer therapeutic bispecific diabodies expressed in Escherichia coli2020

    • Author(s)
      Hikaru Nakazawa, Tomoko Onodera-Sugano, Aruto Sugiyama, Yoshikazu Tanaka, Takamitsu Hattori, Teppei Niide, Hiromi Ogata, Ryutaro Asano, Izumi Kumagai, Mitsuo Umetsu
    • Journal Title

      Biochemical Engineering Journal

      Volume: 160 Pages: 1-9

    • DOI

      10.1016/j.bej.2020.107636

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional Domain Order of an Anti-EGFR × Anti-CD16 Bispecific Diabody Involving NK Cell Activation2020

    • Author(s)
      Kuwahara Atsushi、Nagai Keisuke、Nakanishi Takeshi、Kumagai Izumi、Asano Ryutaro
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 23 Pages: 8914-8914

    • DOI

      10.3390/ijms21238914

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sustained anti-osteoporotic action of risedronate compared to anti-RANKL antibody following discontinuation in ovariectomized mice2020

    • Author(s)
      Omiya Toshinobu、Hirose Jun、Omata Yasunori、Tominari Tsukasa、Inada Masaki、Watanabe Hisato、Miyamoto Takeshi、Tanaka Sakae
    • Journal Title

      Bone Reports

      Volume: 13 Pages: 100289-100289

    • DOI

      10.1016/j.bonr.2020.100289

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular evidence of IGFBP-3 dependent and independent VD3 action and its nonlinear response on IGFBP-3 induction in prostate cancer cells2020

    • Author(s)
      Igarashi Ko、Yui Yoshihiro、Watanabe Kenta、Kumai Jun、Nishizawa Yasuko、Miyaura Chisato、Inada Masaki、Sasagawa Satoru
    • Journal Title

      BMC Cancer

      Volume: 20 Issue: 1 Pages: 802-802

    • DOI

      10.1186/s12885-020-07310-5

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Combination of Soy Isoflavones and Resveratrol Preserve Bone Mineral Density in Hindlimb-unloaded Mice.2020

    • Author(s)
      Tousen Y、Ichimaru R、Kondo T、Inada M、Miyaura C、Ishimi Y
    • Journal Title

      Nutrients

      Volume: 12(7) Issue: 7 Pages: 2043-2055

    • DOI

      10.3390/nu12072043

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 筋骨格系の恒常性と機能性因子2023

    • Author(s)
      稲田 全規
    • Organizer
      日本農芸化学会2023年度大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Development of aptamer based electrochemical sensor for the therapeutic single-chain variable fragment bio-process monitoring2023

    • Author(s)
      濵﨑真衣、早出広司、池袋一典、浅野竜太郎
    • Organizer
      電気化学会第90回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 骨髄芽球より分化誘導された好中球によるサイトカイン産生の検討2022

    • Author(s)
      磯村佳奈、富成司、平田美智子、稲田全規
    • Organizer
      第95回日本生化学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 加重力およひ?微小重力下の運動によるマウス筋骨格系への影響2022

    • Author(s)
      稲田 全規
    • Organizer
      日本未病学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Caveolin-3発現が骨格筋細胞に与える影響2022

    • Author(s)
      青木 鋭人、松延 道生、本橋 紀夫、富成 司、稲田 全規、青木 吉嗣
    • Organizer
      第8回日本筋学会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] プロスタグランジンJ2による筋細胞増殖と分化の抑制作用2022

    • Author(s)
      髙戸谷 賢、富成 司、松本 千穂、宮浦 千里、平田 美智子、青木 吉嗣、稲田 全規
    • Organizer
      第8回日本筋学会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 乳癌エクソソームの膜型HB-EGFによるプロスタグランジンE2産生誘導と骨破壊の促進2022

    • Author(s)
      芳之内翔成、唐牛健杜、菅崎萌、山崎徳道、富成司、平田美智子、丸山隆幸、稲田全規
    • Organizer
      第40回日本骨代謝学会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 多重特異性抗体の開発トレンド2022

    • Author(s)
      浅野竜太郎
    • Organizer
      JBA創薬モダリティ基盤研究会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] タンパク質工学を駆使した多重特異性抗体の開発2022

    • Author(s)
      浅野竜太郎
    • Organizer
      第74回日本生物工学会大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] グルコース脱水素酵素のスプリット化と電気化学イムノセンシング素子としての応用2022

    • Author(s)
      飛田悠花、平野健輔、早出広司、津川若子、池袋一典、浅野竜太郎
    • Organizer
      2022年 電気化学秋季大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 低分子抗体と乳酸酸化酵素を用いた四価イムノセンシング素子の開発2022

    • Author(s)
      織田美帆、平賀健太郎、早出広司、津川若子、池袋一典、浅野竜太郎
    • Organizer
      日本化学会 生体機能関連化学部会若手の会 第33回サマースクール
    • Related Report
      2022 Annual Research Report
  • [Presentation] 核酸アプタマー:抗体:二酵素協奏型迅速簡便ウイルス検出系の構築2022

    • Author(s)
      三浦大明
    • Organizer
      日本化学会 生体機能関連化学部会若手の会 第33回サマースクール
    • Related Report
      2022 Annual Research Report
  • [Presentation] Development of anti-single-chain variable fragments DNA aptamers for the therapeutic scFv sensing2022

    • Author(s)
      Mai Hamasaki, Ryutaro Asano, Kazunori Ikebukuro, Koji Sode
    • Organizer
      ACS Fall Meeting 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of DNA aptamers that specifically recognize single-chain variable fragments2022

    • Author(s)
      Mai Hamasaki, Ryutaro Asano, Kazunori Ikebukuro, Koji Sode
    • Organizer
      BMES Annual Meeting
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 低分子二重特異性抗体の機能改変を目指したブレビバチルス菌表層提示2022

    • Author(s)
      中原維新, 大室朋海, 浅野竜太郎
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 配向性の異なる二重特異性抗体の原子間力顕微鏡を用いた細胞間架橋能評価2021

    • Author(s)
      前島 敦, 石橋健太, 金賢徹, 熊谷 泉, 浅野竜太郎
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] インターロイキン2 (IL-2) の融合による二重特異性がん治療抗体の高機能化2021

    • Author(s)
      廣野琳子, 浅野竜太郎, 熊谷泉, 池袋一典
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 抗体酵素複合体を利用したヘモグロビンの迅速簡便かつ高感度な電気化学測定手法の開発2021

    • Author(s)
      三浦大明, 木村勇斗, 津川若子, 早出広司, 池袋一典, 浅野竜太郎
    • Organizer
      2021年電気化学会秋季年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 低分子抗体と乳酸酸化酵素を用いた電気化学イムノセンシング素子の開発2021

    • Author(s)
      織田美帆, 平賀健太郎, 塚本夏海, 早出広司, 津川若子, 池袋一典, 浅野竜太郎
    • Organizer
      2021年電気化学会秋季年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Convenient and universal fabrication of high affinity bispecific bivalent antibody-enzyme complex as sensing element using two Catcher/Tag systems2021

    • Author(s)
      Hayato Kimura, Ryutaro Asano, Wakako Tsugawa, Kazunori Ikebukuro, Koji Sode
    • Organizer
      BIOSENSORS 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 二重特異性抗体医薬の開発: これまでとこれから2021

    • Author(s)
      浅野竜太郎
    • Organizer
      第37回日本DDS学会学術集会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] LC-MSによるヒト骨格筋ジストロフィンの高精度タンパク質定量法の開発2021

    • Author(s)
      富成 司、松延 道生、髙戸谷 賢、平田 美智子、武田 伸一、青木 吉嗣、稲田 全規
    • Organizer
      第7回日本筋学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 廃用性筋萎縮における筋形成系および筋分解系因子の時系列的な発現変動2021

    • Author(s)
      髙戸谷 賢、富成 司、田中 優樹、平田 美智子、青木 吉嗣、稲田 全規
    • Organizer
      第7回日本筋学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 微小重力および加重力による マウス筋骨格系形成・萎縮の制御2021

    • Author(s)
      稲田 全規
    • Organizer
      第67回日本宇宙航空環境医学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 前立腺癌誘導性の骨吸収におけるエクソソーム膜型HB-EGFの関与2021

    • Author(s)
      芳之内 翔成、富成 司、平田 美智子、稲田 全規
    • Organizer
      第39回日本骨代謝学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 乳癌による血管新生と骨破壊におけるエクソソーム膜型HB-EGFの関与2021

    • Author(s)
      山崎 徳道 、芳之内 翔成、平田 美智子、丸山 隆幸、稲田 全規
    • Organizer
      第39回日本骨代謝学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ヒト上皮増殖因子受容体のセンシングを目指した乳酸酸化酵素融合抗体の開発と機能評価2021

    • Author(s)
      織田美帆, 平賀健太郎, 塚本夏海, 早出広司, 津川若子, 池袋一典, 浅野竜太郎
    • Organizer
      電気化学会第88回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] アントシアニジンによる炎症性骨吸収の抑制作用と構造活性相関2021

    • Author(s)
      磯村 佳奈、富成 司、平田 美智子、宮浦 千里、稲田 全規
    • Organizer
      第17回ファンクショナルフード学会 学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 柑橘の機能性表示食品開発と質量分析イメージング解析2021

    • Author(s)
      芳之内  翔成、富成 司、平田 美智子、宮浦 千里、稲田 全規
    • Organizer
      第17回ファンクショナルフード学会 学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Development of novel quantitative method to evaluate cross-linking ability of bispecific antibodies using atomic force microscopy.2020

    • Author(s)
      Atsushi Maejima, Kenta Ishibashi, Hyonchol Kim, Kazunori Ikebukuro, Izumi Kumagai, Ryutaro Asano,
    • Organizer
      第20回日本蛋白質科学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Development Of Novel Preparation Method For Bispecific Antibody Using Dimerizing Motif And Refolding Procedure2020

    • Author(s)
      Linko Hirono, Kazunori Ikebukuro, Izumi Kumagai, Ryutaro Asano
    • Organizer
      第20回日本蛋白質科学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 原子間力顕微鏡を用いたがん細胞傷害活性の異なる二重特異性抗体の細胞間架橋能評価2020

    • Author(s)
      前島 敦, 石橋健太, 池袋一典, 金 賢徹, 熊谷 泉, 浅野竜太郎
    • Organizer
      日本化学会第101回春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シアノバクテリアを用いた低分子二重特異性がん治療抗体の発現量の向上を目指した検討2020

    • Author(s)
      葛西豪太, 池袋一典, 早出広司, 浅野竜太郎
    • Organizer
      日本化学会第101回春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 破骨細胞分化におけるエクソソーム分泌制御因子の関与2020

    • Author(s)
      富成 司、田中 優樹、平田 美智子、宮浦 千里、稲田 全規
    • Organizer
      第38回日本骨代謝学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 悪性黒色腫細胞の膜型リガンドHB-EGF による骨破壊の制御2020

    • Author(s)
      芳之内  翔成、唐牛 健杜、富成  司、平田 美智子、宮浦 千里、稲田 全規
    • Organizer
      第38回日本骨代謝学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 前立腺癌誘導性の骨形成におけるIL-1 シグナリングの関与2020

    • Author(s)
      宮川 萌、芳之内  翔成、平田  美智子、宮浦 千里、稲田 全規
    • Organizer
      第38回日本骨代謝学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] プロスタグランジンE2による筋管形成の促進作用2020

    • Author(s)
      富成 司、篠崎 靖子、平田 美智子、武田 伸一、青木 吉嗣、宮浦 千里、稲田 全規
    • Organizer
      第6回日本筋学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] スパイクインアプローチによるジストロフィンタンパク質の定量方法2020

    • Author(s)
      髙戸谷 賢、富成 司、松延 道生、平田 美智子、宮浦 千里、武田 伸一、青木 吉嗣、稲田 全規
    • Organizer
      第6回日本筋学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Caveolin-3発現が骨格筋細胞に与える影響2020

    • Author(s)
      松延 道生、本橋 紀夫、稲田 全規、宮浦 千里、青木 吉嗣
    • Organizer
      第6回日本筋学会学術集会
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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