| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2022: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2021: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2020: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Outline of Final Research Achievements |
The TOM complex was purified from a yeast overexpressing strain. HS-AFM analysis demonstrated that the purified TOM complex forms a trimeric structure in solution and tends to dissociate into a stable core-dimer and a peripheral monomer. Furthermore, we succeeded in real-time imaging of interaction between the TOM complex and mitochondrial precursor proteins, in which a substrate protein is added to the TOM complex under HS-AFM observation. We are currently attempting to identify the substrate-binding region of the TOM complex by applying various substrates.
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