• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Three dimensional thermal imaging in vivo for thermobiology

Research Project

Project/Area Number 20H02586
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 28040:Nanobioscience-related
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Kamei Yasuhiro  基礎生物学研究所, 超階層生物学センター, RMC教授 (70372563)

Co-Investigator(Kenkyū-buntansha) 野中 茂紀  大学共同利用機関法人自然科学研究機構(新分野創成センター、アストロバイオロジーセンター、生命創成探究, 生命創成探究センター, 准教授 (90435529)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2022: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywords温度 / 熱 / イメージング / 温度計測 / 局所加熱 / 熱ショック応答 / HSF / 赤外レーザー / 単一細胞 / biothermology / バイオサーモロジー / 熱動態解析 / 高速イメージング / 分子進化
Outline of Research at the Start

生命科学で扱いが曖昧な物理量として「温度(熱)」がある。数年前から「細胞内に温度分布がある」との報告がある一方で物理的に「ありえない」との疑義も上がっている。細胞内での熱の振舞いを理解するためには、細胞内の熱伝導率を知る必要がある。そこで、本研究では赤外レーザーを用いて細胞内局所の加熱を行い、その摂動による熱拡散を高速温度イメージング技術で捕捉する。そして、分子動力学シミュレーションで細胞内熱伝導率マップを作成し、細胞が熱をどのように利用しているかの可能性を検討する。

Outline of Final Research Achievements

The purpose of this study is to understand what "temperature (heat)" means to life as a physical quantity that is ambiguous in the life sciences. Through understanding the thermal conductivity of biomaterials in cells, we will consider how life utilizes or adapts to heat. To achieve this goal, we used an infrared laser to capture thermal diffusion through perturbations caused by intracellular local heating using a new imaging technique. For temperature measurement, a novel fluorescent protein probe was developed in collaboration with a collaborator, Prof. Nagai (Osaka University). We also performed heating experiments using a cultured cell line expressing the probe, and obtained the thermal conductivity of the cytoplasm by analyzing heat conduction and published the results in a paper (Lu et al. Nano Letters 2022).

Academic Significance and Societal Importance of the Research Achievements

これまで基礎生物学分野で手を付けられていなかった温度や熱を議論するための基盤的なデータを収集することのできる計測法がある程度確立できた。今後より詳細に生体物質の熱特性データを集めることで、生物がどのように熱を活用あるいは、温度に対して適応して来たのかを議論できるようになり、生物ならびにその進化をより深く理解できるようになると思われる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (20 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (2 results) Journal Article (11 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 11 results,  Open Access: 8 results) Presentation (3 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Book (1 results) Remarks (3 results)

  • [Int'l Joint Research] Texas University(米国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Mackey Medical Collage(その他の国・地域(台湾))

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Targeted single-cell gene induction by optimizing the dually regulated CRE/loxP system by a newly defined heat-shock promoter and the steroid hormone in Arabidopsis thaliana2023

    • Author(s)
      Takumi Tomoi, Toshiaki Tameshige, Eriko Betsuyaku, Saki Hamada, Joe Sakamoto, Naoyuki Uchida, Keiko U Torii, Kentaro K Shimizu, Yosuke Tamada, Hiroko Urawa, Kiyotaka Okada, Hiroo Fukuda, Kiyoshi Tatematsu, Yasuhiro Kamei, Shigeyuki Betsuyaku
    • Journal Title

      Frontiers in Plant Science

      Volume: in press Pages: 1171531-1171531

    • DOI

      10.3389/fpls.2023.1171531

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer2022

    • Author(s)
      Lu Kai、Wazawa Tetsuichi、Sakamoto Joe、Vu Cong Quang、Nakano Masahiro、Kamei Yasuhiro、Nagai Takeharu
    • Journal Title

      Nano Letters

      Volume: 22 Issue: 14 Pages: 5698-5707

    • DOI

      10.1021/acs.nanolett.2c00608

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The pentameric hydrocoel lobes organize adult pentameral structures in a sea cucumber, Apostichopus japonicus2022

    • Author(s)
      Udagawa Sumio、Nagai Akiko、Kikuchi Mani、Omori Akihito、Tajika Atsushi、Saito Mieko、Miura Toru、Irie Naoki、Kamei Yasuhiro、Kondo Mariko
    • Journal Title

      Developmental Biology

      Volume: 492 Pages: 71-78

    • DOI

      10.1016/j.ydbio.2022.09.002

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mosaic gene expression analysis of semaphorin-plexin interactions in Caenorhabditis elegans using the IR-LEGO single-cell gene induction system2022

    • Author(s)
      M. Suzuki, A. Nukazuka, Y. Kamei, S. Yuba, Y. Oda, S. Takagi
    • Journal Title

      Development, Growth and Differentiation

      Volume: 64 Issue: 5 Pages: 12793-12793

    • DOI

      10.1111/dgd.12793

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Temperature elevation was detected in migrating cells2022

    • Author(s)
      Nakamura T, Sakamoto J, Okabe K, Taniguchi A, Yamada T G, Nonaka S, Kamei Y, Funahashi A, Tominaga M, Hiroi N F
    • Journal Title

      Optics Continuum

      Volume: -

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a screening system for agents that modulate taste receptor expression with the CRISPR-Cas9 system in medaka2022

    • Author(s)
      Beppu Kana、Tsutsumi Rie、Ansai Satoshi、Ochiai Nana、Terakawa Mai、Mori Marie、Kuroda Masashi、Horikawa Kazuki、Tomoi Takumi、Sakamoto Joe、Kamei Yasuhiro、Naruse Kiyoshi、Sakaue Hiroshi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 601 Pages: 65-72

    • DOI

      10.1016/j.bbrc.2022.02.082

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of a novel siphonaxanthin biosynthetic precursor in Codium fragile that accumulates only by exposure to blue-green light2022

    • Author(s)
      Soichiro Seki, Yumiko Yamano, Naohiro Oka, Yasuhiro Kamei, Ritsuko Fujii
    • Journal Title

      FEBS Letters

      Volume: - Issue: 12 Pages: 1544-1555

    • DOI

      10.1002/1873-3468.14357

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mosaic gene expression analysis of semaphorin-plexin interactions in Caenorhabditis elegans using the IR-LEGO single-cell gene induction system2022

    • Author(s)
      Motoshi Suzuki, Akira Nukazuka, Yasuhiro Kamei, Shunsuke Yuba, Yoichi Oda, Shin Takagi
    • Journal Title

      Development, Growth and Differentiation

      Volume: -

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An easy and rapid staining method for confocal microscopic observation and reconstruction of three‐dimensional images of echinoderm larvae and juveniles2021

    • Author(s)
      Tokanai Kohei、Kamei Yasuhiro、Minokawa Takuya
    • Journal Title

      Development, Growth and Differentiation

      Volume: 63 Issue: 9 Pages: 478-487

    • DOI

      10.1111/dgd.12758

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Injury-induced Cavl-expressing cells at lesion rostral side play major roles in spinal cord regeneration2021

    • Author(s)
      Zeng Chih-Wei、Kamei Yasuhiro、Shigenobu Shuji、Sheu Jin-Chuan、Tsai Huai-Jen
    • Journal Title

      Open Biology

      Volume: 11 Issue: 2 Pages: 200304-200304

    • DOI

      10.1098/rsob.200304

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Light-sheet microscopy-based 3D single-cell tracking reveals a correlation between cell cycle and the start of endoderm cell internalization in early zebrafish development2020

    • Author(s)
      Kondow Akiko、Ohnuma Kiyoshi、Kamei Yasuhiro、Taniguchi Atsushi、Bise Ryoma、Sato Yoichi、Yamaguchi Hisateru、Nonaka Shigenori、Hashimoto Keiichiro
    • Journal Title

      Development, Growth & Differentiation

      Volume: 62 Issue: 7-8 Pages: 495

    • DOI

      10.1111/dgd.12695

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] An introduction to infrared laser mediated single-cell gene induction method in vivo, and an application of the same system for a new biological field, biothermology.2022

    • Author(s)
      Yasuhiro Kamei
    • Organizer
      TTBA Webinar (Texas University)
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Application of infrared laser to living cells for manipulation of gene expression, and in vivo temperature measurement method2021

    • Author(s)
      Takumi Tomoi, Joe Sakamoto, Suguru Ohe, Yosuke Tamada, Yasuhiro Kamei
    • Organizer
      Optics & Photonics International Congress 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 共同利用による顕微鏡/イメージング技術提供2021

    • Author(s)
      亀井保博
    • Organizer
      再生学異分野融合研究会(第3回)オンライン
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Book] 達人に訊くバイオ画像取得と定量解析Q&A2021

    • Author(s)
      加藤 輝、小山 宏史(編)、亀井保博、坂本丞、野中茂紀、村田隆、谷口篤史
    • Total Pages
      220
    • Publisher
      羊土社
    • ISBN
      9784758122504
    • Related Report
      2020 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      https://sites.google.com/nibb.ac.jp/bio-imaging/kamei-lab

    • Related Report
      2022 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      https://www.nibb.ac.jp/lspectro/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      https://www.nibb.ac.jp/lspectro/kamei_lab.html

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi