| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2023: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2022: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2021: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
To visualize chemical reactions associated with structural changes in a wide range of proteins, time-resolved resonance Raman spectroscopic methods were developed using ultrafast molecular photoswitching. The photoreaction of a small molecule, such as photoisomerization of azobenzene introduced into the protein backbone, was used for switching to form three-dimensional structures of targeted proteins. We did not obtain sufficient amount of photoswitching protein samples for spectroscopic measurements. The time-resolved spectroscopy system was improved from the existing system. We demonstrated that the performance of the spectroscopic system was sufficient to follow the chemical reactions of targeted proteins.
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