| Project/Area Number |
20H02776
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 34030:Green sustainable chemistry and environmental chemistry-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
FOX BRIAN 北海道大学, 農学研究院, 教授 (90812397)
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| Co-Investigator(Kenkyū-buntansha) |
高須賀 太一 北海道大学, 農学研究院, 准教授 (70748409)
栗原 秀幸 北海道大学, 水産科学研究院, 教授 (40234570)
細川 雅史 北海道大学, 水産科学研究院, 教授 (10241374)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2023: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2022: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | enzymatic hydrolysis / alginate / laminarin / fukoidan / health benefit / Kelp hydrolysis / Alginate / Laminarin / kelp hydrolysis / GH55 enzyme / PL enzymes / Kelp hydrolysate / kelp-degrading enzymes / Enzyme kelp hydrolysis / Chemical composition / Genome-enabled biochem / kelp for biofuels / kelp for health benefits |
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| Outline of Research at the Start |
We will use advanced genome-science methods to identify enzymes capable of efficient and rapid hydrolysis of all polysaccharides contained in abundant, renewable, and flammable Japanese brown kelp species. The monosaccharide products obtained by this work have potential great benefits as source for microbial fermentation into a myriad of biofuels and byproducts useful to our society. Furthermore, successful extraction of a newer intact bioactive polysaccharides would provide great health benefits to human health, with its antioxidant, anti-inflammatory, or other properties.
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| Outline of Annual Research Achievements |
To achieve an effective enzyme combination for enzymatic hydrolysis of laminarin and alginate in kelp, a glycoside hydrolase family 55 enzyme (SactELam55A) and two polysaccharide enzyme families (PLs), seven PL17 and two PL18 were successfully prepared using a recombinant enzyme production method. The reaction optima of temperature and pH of these enzymes were individually determined for combinatorial enzyme hydrolysis. Additionally, one reported PL7 enzyme was gene synthesized to produce monomer from alginate polymer in combination with PL17 and PL18 alginate depolymerizing enzymes.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Four classes of kelp depolymerizing enzymes have been produced and determined their reaction optima and ready for kelp hydrolysis. Also, four different kelp species have been collected and fractionated for enzyme-assisted kelp hydrolysis. We are a bit behind in terms of publishing the first paper for kelp hydrolysis using a combination of two enzymes, SactELam55A and PL18.
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| Strategy for Future Research Activity |
All enzymes will be subjected for combinatorial kelp hydrolysis to determine the most efficient enzyme combination under reaction optima (Temperature and pH). Kelp will be hydrolyzed by the optimum enzymatic hydrolysis, and hydrolysates will be analyzed by analytical methods using HPLC and GC/MS. We will also fractionate hydrolysate to determine end products. Fractionated samples will be tested for their health beneficial functions.
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