| Project/Area Number |
20H02776
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 34030:Green sustainable chemistry and environmental chemistry-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
Fox Brian 北海道大学, 農学研究院, 教授 (90812397)
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| Co-Investigator(Kenkyū-buntansha) |
高須賀 太一 北海道大学, 農学研究院, 准教授 (70748409)
栗原 秀幸 北海道大学, 水産科学研究院, 教授 (40234570)
細川 雅史 北海道大学, 水産科学研究院, 教授 (10241374)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2024)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2023: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2022: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | glycoside hydrolase 55 / polysaccharide lyase 18 / polysaccharide lyase 17 / kelp / alginate lyase / PL17 / Kelp hydrolysis / enzymatic hydrolysis / alginate / laminarin / fukoidan / health benefit / Alginate / Laminarin / kelp hydrolysis / GH55 enzyme / PL enzymes / Kelp hydrolysate / kelp-degrading enzymes / Enzyme kelp hydrolysis / Chemical composition / Genome-enabled biochem / kelp for biofuels / kelp for health benefits |
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| Outline of Research at the Start |
We will use advanced genome-science methods to identify enzymes capable of efficient and rapid hydrolysis of all polysaccharides contained in abundant, renewable, and flammable Japanese brown kelp species. The monosaccharide products obtained by this work have potential great benefits as source for microbial fermentation into a myriad of biofuels and byproducts useful to our society. Furthermore, successful extraction of a newer intact bioactive polysaccharides would provide great health benefits to human health, with its antioxidant, anti-inflammatory, or other properties.
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| Outline of Final Research Achievements |
In this project, we focused on discovering novel enzyme combinations to efficiently decompose major polysaccharides in kelp, such as laminarin and alginate. A major laminarin-degrading enzyme was previously reported from our group, which produces glucose from laminarin (Bianchetti et al., J. Biol. Chem., 2015), and we determined the best acting alginate-degrading enzymes in the polysaccharide lyase family 18 (PL18) in the CaZymes classification. GH55 from Streptomyces sp. SirexAA-E, SactELam55A, and CaPL18A from Catenovulum agarivorans efficiently decompose actual kelp material (Takasuka et al., ChemBioChem, 2023). Furthermore, we have explored a wider range of alginate lyase families, including PL17 and PL34. The former is suggested to be an exo-acting alginate lyase family, and the latter is a poorly characterized family. From the detailed functional characterization of one of the PL17s from Saccharobesus litoralis CCB-QB4, we determined a novel function of this enzyme
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| Academic Significance and Societal Importance of the Research Achievements |
Kelp is a farmable resource available along a coast in Japan. However, knowledge of enzyme-based kelp degradation is limited compared to that for terrestrial biomass. Thus, exploring and discovering novel enzymes for kelp degradation will contribute to future sustainable energy production.
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