Understanding Cellular Function with Short RNAs and Small Molecules
| Project/Area Number |
20H02859
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 37010:Bio-related chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
SATO SHINICHI 京都大学, 化学研究所, 准教授 (70534478)
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| Co-Investigator(Kenkyū-buntansha) |
萩原 正規 弘前大学, 理工学研究科, 准教授 (40403000)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2022: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2021: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2020: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 機能性RNA / 小分子化合物 / ケミカルバイオロジー / イメージング / 核酸 |
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| Outline of Research at the Start |
複雑な細胞機能の理解を深めるためには，タンパク質や核酸などの機能性生体高分子の 時空間的な機能発現を系統立てて研究することが重要だ．しかし，細胞自身が生産する生 体分子を天然のままで解析可能な方法は少なく技術的に難しい．本研究では，採択者が開発した「機能性RNAと小分子化合物」を研究ツールとして利用し，哺乳動物細胞内でRNA機能を操り，タンパク質の機能発現機構を解析できる革新的方法の確立を目指す．
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| Outline of Final Research Achievements |
To understand complex cellular functions, it is important to systematically investigate the spatio-temporal functional expression of bioorganic molecules such as proteins and nucleic acids. However, there are few methods that allow analysis of endogenous bioorganic molecules in living cells, making it technically difficult. In this research, we established an innovative method to regulate RNA functions in mammalian cells and to analyze gene expression by using "a functional RNA and a small molecule" as a research tool.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では生細胞内でRNA機能を操る新たな研究手法を創出し、未知の生命現象を解き明かす研究ツールとして利用することを目指した。研究期間内で大きな成果を得ることに成功し、国際的評価が最も高い核酸化学専門誌であるNucleic Acids Research誌（IF＝19.160）への２報の報告を含む、４報への国際誌に研究成果を報告した。
Selected Publications
1. Nucleic Acids Research,50(14),8143-8153,2022, 2. Nucleic Acids Research,49(22),e132-e132,2021
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Small molecule-based detection of non-canonical RNA G-quadruplex structures that modulate protein translation2022
Author(s)
Yousuke Katsuda, Shin-ichi Sato, Maimi Inoue, Hisashi Tsugawa, Takuto Kamura, Tomoki Kida, Rio Matsumoto, Sefan Asamitsu, Norifumi Shioda, Shuhei Shiroto, Yoshiki Oosawatsu, Kenji Yatsuzuka, Yusuke Kitamura, Masaki Hagihara, Toshihiro Ihara and Motonari Uesugi
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Journal Title
Nucleic Acids Research
Volume: 50
Issue: 14
Pages: 8143-8153
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Glucose as a Protein-Condensing Cellular Solute2022
Author(s)
Noda, N., Jung, Y., Ado, G., Mizuhata, Y., Higuchi, M., Ogawa, T., Ishidate, F., Sato, S., Kurata, H., Tokitoh, N., Uesugi, M.
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Journal Title
ACS Chem. Biol.
Volume: 17
Issue: 3
Pages: 567-575
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Discovery of Non-Cysteine-Targeting Covalent Inhibitors by Activity-Based Proteomic Screening with a Cysteine-Reactive Probe2022
Author(s)
Jung, Y., Noda, N., Takaya, J., Abo, M., Toh, K., Tajiri, K., Cui, C., Zhou, L., Sato, S., Uesugi, M.
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Journal Title
ACS Chem. Biol.
Volume: 17
Issue: 2
Pages: 340-347
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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