| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2022: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2021: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Outline of Final Research Achievements |
In this research, I proposed a new method for designing molecular probes for super-resolution mapping of endogenous proteins by extending the design method of probes for chemical tag technology that I have independently developed in recent years. I developed small-molecular probes optimized for such as AMPA-type glutamate receptor and tubulin, and applied these probes to living cells to enable fluorescence imaging of endogenous functional proteins. Furthermore, microstructures that cannot be resolved by confocal microscopy were successfully visualized over time by super-resolution mapping using STED microscopy.
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