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Conversion of legume crops to low carbon input lines using nitrogen fixation enhancing genes

Research Project

Project/Area Number 20H02890
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionSaga University

Principal Investigator

Suzuki Akihiro  佐賀大学, 農学部, 教授 (50305108)

Co-Investigator(Kenkyū-buntansha) 渡邊 啓史  佐賀大学, 農学部, 准教授 (40425541)
富永 晃好  静岡大学, 農学部, 助教 (50776490)
下村 彩  佐賀大学, 農学部, 助教 (20802771)
遠城 道雄  鹿児島大学, 農水産獣医学域農学系, 教授 (60194651)
穴井 豊昭  佐賀大学, 農学部, 招へい教授 (70261774)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2023: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2022: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords根粒菌 / 窒素固定 / ダイズ / 収量 / 遺伝子 / 共生 / ミヤコグサ / 根粒
Outline of Research at the Start

申請者らは,根粒菌による生物的窒素固定を飛躍的に増強させ,窒素肥料の使用(製造)を大幅に削減することを目標として研究を推進してきた。そして今までに,ダイズ品種エンレイから窒素固定増強遺伝子(エンレイ型SEN1)を同定し,それをフクユタカへ導入することによって窒素固定活性をほぼ100%増強させることに成功した。そこで本研究では,①ダイズの窒素固定増強遺伝子による窒素肥料削減効果の検証,②ダイズの窒素固定増強遺伝子のリスト化と世界展開への準備,③SEN1タンパク質の機能解析の3つの小課題を遂行し,窒素固定増強のメカニズムを理解するとともに,低炭素投入型農業が実現可能であることを示す。

Outline of Final Research Achievements

In this research, we used a FUKUYUTAKA SEN1 line (Enrei type) and a conventional line with the SEN1 gene (Peking type), and conducted three research projects: (1) verification of the effect of the Enrei type on nitrogen fertilizer reduction, (2) the effect of the Enrei type on seed yield, and (3) functional analysis of the SEN1 protein. As a result, it was found that (1) the Enrei type showed the same growth as the Peking type even when nitrogen fertilizer was reduced to half. In case (2), the nitrogen fixation enhanced SEN1 showed a tendency to increase yield more than the Peking type in nitrogen poor condition. In (3), the higher concentration of iron in the nodules of the Enrei type supported the possibility that the SEN1 protein is involved in iron transport.

Academic Significance and Societal Importance of the Research Achievements

ダイズにおける窒素固定増強遺伝子を同定し,それを日本で最も栽培面積の大きな品種(フクユタカ)に導入し,その性状解析を行った点は,学術的に大きな意義がある。また得られた結果は,ダイズ栽培においてこの遺伝子を用いれば,窒素肥料の半分程度までの低減を達成できる可能性を示すものであり,肥料価格が高騰している現代社会において非常に大きなインパクトのある成果だと考えられる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (9 results)

All 2024 2023 2022 2021 2020 Other

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (5 results) (of which Int'l Joint Research: 3 results) Remarks (1 results)

  • [Journal Article] “Effect of nitrogen fixation enhancing tEffect of nitrogen fixation enhancing type SEN1 gene on soybean growth2024

    • Author(s)
      Aya Shimomura, Yuki Nishida, Shion Yamamoto, Norio Suganuma, Satoshi Watanabe, Toyoaki Anai, Susumu Arima, Akiyoshi Tominaga, Akihiro Suzuki
    • Journal Title

      PLANT PRODUCTION SCIENCE

      Volume: 27 Issue: 2 Pages: 137-149

    • DOI

      10.1080/1343943x.2024.2326643

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of Inoculating the Diazotrophic Endophyte Bradyrhizobium sp. AT1 on Different Cultivars of Sweet Potato (Ipomoea batatas [L.] Lam.)2023

    • Author(s)
      Junko Terakado-Tonooka, Fukuyo Tanaka, Toshihiko Karasawa, Akihiro Suzuki, Yoshinari Ohwaki
    • Journal Title

      Agronomy

      Volume: 13 Issue: 4 Pages: 963-972

    • DOI

      10.3390/agronomy13040963

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] SEN1 gene from Lotus japonicus MG20 improves nitrogen fixation and plant growth2020

    • Author(s)
      Nishida Yuki、Hiraoka Reona、Kawano Satomi、Suganuma Norio、Sato Shusei、Watanabe Satoshi、Anai Toyoaki、Arima Susumu、Tominaga Akiyoshi、Suzuki Akihiro
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 66 Issue: 6 Pages: 864-869

    • DOI

      10.1080/00380768.2020.1834829

    • NAID

      210000158611

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ダイズ窒素固定増強遺伝子の生育および収量への効果2024

    • Author(s)
      下村彩,西田雄輝,山本士温,菅沼教生,渡邊啓史,穴井豊昭,有馬進,富永晃好,鈴木章弘
    • Organizer
      日本作物学会講演会
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 窒素固定増強ダイズ品種の作出2023

    • Author(s)
      下村彩
    • Organizer
      第255回日本作物学会講演会
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 窒素固定増強遺伝子を導入したダイズの表現型2022

    • Author(s)
      鈴木章弘
    • Organizer
      動物植物生態九州支部三学会合同佐賀大会2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 窒素固定増強遺伝子を導入したフクユタカの優位性2022

    • Author(s)
      鈴木章弘
    • Organizer
      日本作物学会第253回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 窒素固定増強遺伝子を導入したフクユタカの性状解析2021

    • Author(s)
      西田雄輝
    • Organizer
      日本土壌肥料学会2021年度北海道大会
    • Related Report
      2021 Annual Research Report
  • [Remarks]

    • URL

      http://azuki.ag.saga-u.ac.jp/

    • Related Report
      2023 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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