• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Multi-omics analysis of dietary microRNAs and metabolites toward functional food pairing

Research Project

Project/Area Number 20H02935
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38050:Food sciences-related
Research InstitutionKyushu University

Principal Investigator

Fujimura Yoshinori  九州大学, 農学研究院, 准教授 (20390304)

Co-Investigator(Kenkyū-buntansha) 三浦 大典  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (40532627)
立花 宏文  九州大学, 農学研究院, 教授 (70236545)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2021: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords機能性フードペアリング / 代謝物 / マイクロRNA / オミクス解析 / センシング / 機能性ペアリング
Outline of Research at the Start

本研究は、食機能を厳密に読み解き、機能を享受できる食べ合せを提示できる新たな概念・方法論を見出すことを主眼としている。単一の含有成分情報(量や効果の強さ)のみで機能性の良し悪しが判断されている現状の機能性食品(特定保健用食品や機能性表示食品)開発における絶対的価値観とは一線を画するものであり、食機能が複合成分の協調的な組合せ効果により顕在化するとの独自コンセプトの下、機能性発現に有効な成分の組合せに基づいた食べ合わせ「機能性フードペアリング」を提示できる革新的成分評価技術の創出に挑む研究である。

Outline of Final Research Achievements

From the viewpoint that food functionality is based on the cooperative action of a series of components, we verified the multifaceted interrelationships among components that contribute to "functional food pairing," a combination of food that enables us to receive the functionality that manifests when specific components are combined. Through metabolic profiling analysis using a green tea extract library, we were able to propose functionality-related polyphenol components and useful combinations of components that enhance functionality. In addition, we discovered novel signal molecules capable of sensing functional components for effectively extracting the potential combinations. We also identified novel biomarkers that contribute to risk assessment of functional components. Furthermore, we identified various microRNAs as candidates of functional components in green tea, and clarified the functionality of plant-derived microRNAs.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、機能性を享受できる食べ合わせ「機能性フードペアリング」を見出すことができる新規概念・方法論の創出に寄与する。単一の含有成分情報(量や効果の強さ)のみを機能性評価基準とした従来型研究とは一線を画し、重要性が認知されながらも複雑系であるために実施困難な「機能性に影響しうる複数の共存成分を考慮した食機能解明研究」を加速化させる学術的インパクトを持つ。食機能に紐づく成分の複雑性を解きほぐし、食品成分・素材の組合せ効果の理解(科学的根拠の提示)や予測・判別、成分の有効活用に資する機能性デザインフードの戦略的立案に役立つ成分評価のブレークスルー技術を提案するものである。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (32 results)

All 2023 2022 2021 2020

All Journal Article (15 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 15 results,  Open Access: 13 results) Presentation (17 results)

  • [Journal Article] Bioactivity-boosting strategy based on combination of anti-allergic O-methylated catechin with a Citrus flavanone, hesperetin.2023

    • Author(s)
      Fujimura Y, Yoshimoto T, Fujino K, Nezu A, Marugame Y, Bae J, Kumazoe M, Tachibana H.
    • Journal Title

      J. Nat. Med.

      Volume: 77 Issue: 2 Pages: 363-369

    • DOI

      10.1007/s11418-022-01668-5

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plant miRNA osa-miR172d-5p suppressed lung fibrosis by targeting Tab1.2023

    • Author(s)
      Kumazoe M, Ogawa F, Hikida A, Shimada Y, Yoshitomi R, Watanabe R, Onda H, Fujimura Y, Tachibana H.
    • Journal Title

      Sci. Rep.

      Volume: 13 Issue: 1 Pages: 2128-2128

    • DOI

      10.1038/s41598-023-29188-6

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 67-kDa Laminin Receptor-Mediated Cellular Sensing System of Green Tea Polyphenol EGCG and Functional Food Pairing.2022

    • Author(s)
      Fujimura Y, Kumazoe M, Tachibana H.
    • Journal Title

      Molecules

      Volume: 27 Issue: 16 Pages: 5130-5130

    • DOI

      10.3390/molecules27165130

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Myricetin improve cognitive function in SAMP8 mice accompanied with upregulation of BDNF and NGF2022

    • Author(s)
      Yu Shimada, Yuka Sato, Motofumi Kumazoe, Ryo Kitamura, Yoshinori Fujimura, and Hirofumi Tachibana
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 616 Pages: 33-40

    • DOI

      10.1016/j.bbrc.2022.05.039

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metabolic profiling for evaluating the dipeptidyl peptidase-IV inhibitory potency of diverse green tea cultivars and determining bioactivity-related ingredients and combinations2022

    • Author(s)
      Yoshinori Fujimura, Mototsugu Watanabe, Tomomi Morikawa-Ichinose, Konatsu Fujino, Mao Yamamoto, Seita Nishioka, Chihiro Inoue, Fumiyo Ogawa, Madoka Yonekura, Akari Nakasone, Motofumi Kumazoe, and Hirofumi Tachibana
    • Journal Title

      J. Agri. Food Chem

      Volume: 70 Issue: 21 Pages: 6455-6466

    • DOI

      10.1021/acs.jafc.2c01693

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inflammatory markers S100A8/A9 and metabolic alteration for evaluating signs of early phase toxicity of anticancer agent treatment2022

    • Author(s)
      Tomomi Morikawa-Ichinose, Yoshinori Fujimura, Motofumi Kumazoe, Hiroaki Onda, Daisuke Miura, Hirofumi Tachibana
    • Journal Title

      Food and Chemical Toxicology

      Volume: 169 Pages: 113421-113421

    • DOI

      10.1016/j.fct.2022.113421

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fustin, a Flavanonol, Synergically Potentiates the Anticancer Effect of Green Tea Catechin Epigallocatechin-3-O-Gallate with Activation of the eNOS/cGMP Axis2022

    • Author(s)
      Kumazoe M, Fujimura Y, Yoshitomi R, Shimada Y, Tachibana H.
    • Journal Title

      J. Agric. Food Chem.

      Volume: 70 Issue: 11 Pages: 3458-3466

    • DOI

      10.1021/acs.jafc.1c07567

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sesame lignans upregulate glutathione S-transferase expression and downregulate microRNA-669c-3p2022

    • Author(s)
      Marugame Y, Takeshita N, Yamada S, Yoshitomi R, Kummazoe M, Fujimura Y, Tachibana H.
    • Journal Title

      Bioscience of Microbiota, Food and Health

      Volume: 41 Issue: 2 Pages: 66-72

    • DOI

      10.12938/bmfh.2021-067

    • NAID

      130008143386

    • ISSN
      2186-3342
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methylated (-)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 52021

    • Author(s)
      Kumazoe M, Takamatsu K, Horie F, Yoshitomi R, Hamagami H, Tanaka H, Fujimura Y, Tachibana H.
    • Journal Title

      Sci. Rep.

      Volume: 11 Issue: 1 Pages: 23101-23101

    • DOI

      10.1038/s41598-021-02346-4

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The combined effect of green tea and α-glucosyl hesperidin in preventing obesity: a randomized placebo-controlled clinical trial2021

    • Author(s)
      Yoshitomi R, Yamamoto M, Kumazoe M, Fujimura Y, Yonekura M, Shimamoto Y, Nakasone A, Kondo S, Hattori H, Haseda A, Nishihira J, Tachibana H.
    • Journal Title

      Sci. Rep.

      Volume: 11 Issue: 1 Pages: 19067-19067

    • DOI

      10.1038/s41598-021-98612-6

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Glucosyl-hesperidin enhances the cyclic guanosine monophosphate-inducing effect of a green tea polyphenol EGCG2021

    • Author(s)
      Kumazoe M, Tanaka Y, Yoshitomi R, Marugame Y, Lee KW, Onda H, Fujimura Y, Yonekura M, Shimamoto Y, Tachibana H.
    • Journal Title

      J. Nat. Med.

      Volume: 75 Issue: 4 Pages: 1037-1042

    • DOI

      10.1007/s11418-021-01538-6

    • NAID

      40022681017

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Eriodictyol-Amplified 67-kDa Laminin Receptor Signaling Potentiates the Antiallergic Effect of O-Methylated Catechin2021

    • Author(s)
      Fujimura Y, Fujino K, Yoshimoto T, Nezu A, Marugame Y, Bae J, Kumazoe M, Tachibana H.
    • Journal Title

      J. Nat. Prod.

      Volume: 84 Issue: 6 Pages: 1823-1830

    • DOI

      10.1021/acs.jnatprod.1c00337

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Time-dependent increase of plasma cGMP concentration followed by oral EGCG administration in mice2021

    • Author(s)
      Tanaka Yasutake、Kumazoe Motofumi、Onda Hiroaki、Fujimura Yoshinori、Tachibana Hirofumi
    • Journal Title

      Food Bioscience

      Volume: 41 Pages: 101017-101017

    • DOI

      10.1016/j.fbio.2021.101017

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Src Mediates Epigallocatechin-3-O-Gallate-Elicited Acid Sphingomyelinase Activation2020

    • Author(s)
      Kumazoe Motofumi、Kadomatsu Mai、Bae Jaehoon、Otsuka Yushi、Fujimura Yoshinori、Tachibana Hirofumi
    • Journal Title

      Molecules

      Volume: 25 Issue: 22 Pages: 5481-5481

    • DOI

      10.3390/molecules25225481

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] EGCG down-regulates MuRF1 expression through 67-kDa laminin receptor and the receptor signaling is amplified by eriodictyol2020

    • Author(s)
      Murata Motoki、Shimizu Yuki、Marugame Yuki、Nezu Ayaka、Fujino Konatsu、Yamada Shuhei、Kumazoe Motofumi、Fujimura Yoshinori、Tachibana Hirofumi
    • Journal Title

      Journal of Natural Medicines

      Volume: 74 Issue: 4 Pages: 673-679

    • DOI

      10.1007/s11418-020-01417-6

    • NAID

      40022344632

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 肝臓特異的67-kDa laminin receptorノックアウトマウスのマルチオミクス解析でひもとく新規肝線維化促進因子.2023

    • Author(s)
      米山拓良, 一瀬智美, 竹下奈津子, 錦戸理紗, 熊添基文, 藤村由紀, 立花宏文.
    • Organizer
      日本農芸化学会2023年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 食品由来マイクロ RNA のコレステロール合成酵素発現調節作用.2022

    • Author(s)
      渡邉凌矢, 井上千尋, 熊添基文, 藤村由紀、立花宏文.
    • Organizer
      日本農芸化学会2022年度西日本支部大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 植物マイクロRNAの脂質代謝関連遺伝子発現調節作用.2022

    • Author(s)
      渡邉凌矢, 井上千尋, 熊添基文, 藤村由紀、立花宏文.
    • Organizer
      第59回化学関連支部合同九州大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 炎症マーカーS100A8/A9を指標とした抗がん剤の早期毒性評価に資する代謝物マーカーの探索.2022

    • Author(s)
      一瀬智美、藤村由紀、熊添基文、恩田宏明、三浦大典、立花宏文.
    • Organizer
      第70回質量分析総合討論会
    • Related Report
      2022 Annual Research Report
  • [Presentation] メタボリック・プロファイリング法を用いた緑茶の抗糖尿病成分の解析.2022

    • Author(s)
      藤村由紀, 渡邉喬嗣, 一瀬智美、山本真生, 熊添基文, 立花宏文.
    • Organizer
      第70回質量分析総合討論会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 植物マイクロ RNA による TGFβシグナル伝達経路抑制を介した肺線維化抑制作用2022

    • Author(s)
      渡邉凌矢, 疋田愛, 小川史代, 島田優, 熊添基文, 藤村由紀, 中曽根光, 米倉円佳, 立花宏文
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] EGCGの抗肥満作用におけるマクロファージの67 k Da ラミニン受容体の関与2021

    • Author(s)
      山本真生, 西岡成汰, 村田京介, 冨岡玲乃, 福富拓哉, 山田脩平, 熊添基文, 藤村由紀,立花宏文
    • Organizer
      第17回日本カテキン年次学術大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] メタボリック・プロファイリング法による緑茶中抗糖尿病成分の解析2021

    • Author(s)
      渡邉喬嗣, 藤村由紀, 中曽根光, 米倉円佳, 立花宏文
    • Organizer
      第26回日本フードファクター学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 抗がん剤の早期毒性評価に向けた炎症マーカーS100A8/A9と代謝物の解析2021

    • Author(s)
      一瀬智美, 藤村由紀, 熊添基文, 三浦大典, 立花宏文
    • Organizer
      第15回メタボロームシンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] 緑茶カテキンEGCGの抗炎症性M2マクロファージ分極促進作用及び67-kDa laminin receptorの関与の解析2021

    • Author(s)
      西岡成汰,小原井春香,冨岡玲乃,村田京介,福富拓哉,山田脩平,牧尾彰子,藤村由紀,立花宏文
    • Organizer
      第75回日本栄養・食糧学会 九州・沖縄支部大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ゴマリグナンによるグルタチオンS-トランスフェラーゼ活性化機構の解明2021

    • Author(s)
      丸亀裕貴, 竹下津子, 山田脩平, 吉富廉, 熊添基文, 藤村由紀,立花宏文
    • Organizer
      2021年度日本農芸化学会西日本・中四国・関西支部合同大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ウーロンホモビスフラバン B の肝臓脂質代謝関連遺伝子発現調節作用におけるマイクロ RNA の関与2021

    • Author(s)
      河田奈摘, 楊琳云, 丸亀裕貴, 吉富廉, 藤村由紀,立花宏文
    • Organizer
      第75回日本栄養・食糧学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 緑茶カテキンEGCGのマウス肝臓におけるサーキュラーRNA発現調節作用2021

    • Author(s)
      吉富廉, 藤村由紀,立花宏文
    • Organizer
      第75回日本栄養・食糧学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 抗がん剤の毒性評価に向けた炎症マーカーS100A8/A9の可能性と代謝物マーカーの探索2021

    • Author(s)
      藤村由紀, 一瀬智美, 熊添基文, 立花宏文
    • Organizer
      第69回質量分析総合討論会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Myricetinの認知機能低下抑制作用とそのメカニズム2021

    • Author(s)
      島田優、佐藤由佳、熊添基文、藤村由紀、立花宏文
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 緑茶品種サンルージュの血液凝固阻害因子TFPI の発現上昇作用に関与する成分の解析2020

    • Author(s)
      渡邉喬嗣、梅晨怡、吉富廉、熊添基文、藤村由紀、立花宏文
    • Organizer
      日本食品科学工学会西日本支部大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] TGFβシグナリングを阻害する植物マイクロRNAの 同定2020

    • Author(s)
      小川史代、疋田愛、熊添基文、藤村由紀、立花宏文
    • Organizer
      2020年度日本フードファクター学会・日本農芸化学会西日本支部合同大会
    • Related Report
      2020 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi