| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2023: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2022: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
ROS bursts are induced by RBOH in the plasma membrane. ROS has been observed as a marker of defense response, but its mechanism of action is not known. ROS sensor protein was purified as a YAP1-binding protein. A wide variety of 102 ROS sensor candidates were obtained. Among these candidates, the recombinant protein of NbGLR was treated with H2O2, then the protein was chemically labeled with a BTD that binds to the sulfenylated cysteine residue. The sulfenylation of the target cysteine residue was confirmed in vitro using a specific antibody.
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