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The molecular basis of the recognition of nematodes by plants

Research Project

Project/Area Number 20H02994
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Kadota Yasuhiro  国立研究開発法人理化学研究所, 環境資源科学研究センター, 専任研究員 (80548975)

Co-Investigator(Kenkyū-buntansha) 植原 健人  国立研究開発法人農業・食品産業技術総合研究機構, 中日本農業研究センター, 産学連携コーディネーター (30355458)
加藤 大明  京都大学, 農学研究科, 特定研究員 (70642635)
望田 啓子 (桑田啓子)  名古屋大学, トランスフォーマティブ生命分子研究所, 特任講師 (70624352)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2021: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords植物寄生線虫 / PAMP / 植物免疫 / ネコブ線虫 / PAMPs / MAMP / 免疫受容体
Outline of Research at the Start

本研究では植物が認識する線虫の物質(MAMPs)を単離するとともに、遺伝学的、及び生化学的アプローチにより、植物の受容体、受容体結合因子、及び情報伝達系の因子を同定する。そして、これらMAMPsの認識が線虫に対する植物免疫に及ぼす影響を解明する。

Outline of Final Research Achievements

This study revealed that the peptide containing the substrate-binding site of Oligosaccharide degrading enzyme from nematodes can induce immunity as MAMP (microbe-associated molecular patterns) in Arabidopsis. The peptide sequence exhibits remarkable conservation among nematode species, including plant parasitic nematodes. Through comprehensive genetic analyses, we have successfully identified a receptor-like kinase as the specific plant receptor for this peptide. Although nematode-derived MAMPs and their recognition systems in plants had been unclear for a very long time, this study shed light on the understanding of the molecular mechanism of nematode recognition by plants.

Academic Significance and Societal Importance of the Research Achievements

植物寄生線虫は世界で最も被害額の大きな病原体の1つであるが、植物による線虫の認識機構については研究が進んでいなかった。本研究により、線虫由来のMAMPsとその認識に必須な植物の受容体キナーゼが同定された。この受容体を足掛かりとして、線虫に対する植物免疫機構の解明や、受容体の活性化を抑制する病原性機構等、様々な研究の発展が期待できる。また、この受容体を作物に導入することにより、線虫認識能力を付与できる可能性もあり、応用研究への発展も期待できる。

Report

(2 results)
  • 2022 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • Research Products

    (10 results)

All 2022 2021 Other

All Int'l Joint Research (3 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Int'l Joint Research] フランス国立農学研究所(INRA)(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] The Sainsbury Laboratory(英国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Zurich(スイス)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] <i>Solanum palinacanthum</i> Dunal as a potential eggplant rootstock resistant to root‐knot nematodes2022

    • Author(s)
      Murata Gaku、Uehara Taketo、Lee Hyoung Jae、Mizutani Masaharu、Kadota Yasuhiro、Shinmura Yoshimi、Saito Takeo、Uesugi Kenta
    • Journal Title

      Journal of Phytopathology

      Volume: 170 Issue: 3 Pages: 185-193

    • DOI

      10.1111/jph.13067

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcriptomic Analysis of Resistant and Susceptible Responses in a New Model Root-Knot Nematode Infection System Using Solanum torvum and Meloidogyne arenaria2021

    • Author(s)
      Sato Kazuki、Uehara Taketo、Holbein Julia、Sasaki-Sekimoto Yuko、Gan Pamela、Bino Takahiro、Yamaguchi Katsushi、Ichihashi Yasunori、Maki Noriko、Shigenobu Shuji、Ohta Hiroyuki、Franke Rochus B.、Siddique Shahid、Grundler Florian M. W.、Suzuki Takamasa、Kadota Yasuhiro、Shirasu Ken
    • Journal Title

      Frontiers in Plant Science

      Volume: 12 Pages: 680151-680151

    • DOI

      10.3389/fpls.2021.680151

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Activation loop phosphorylation of a non-RD receptor kinase initiates plant innate immune signaling2021

    • Author(s)
      Bender Kyle W.、Couto Daniel、Kadota Yasuhiro、Macho Alberto P.、Sklenar Jan、Derbyshire Paul、Bjornson Marta、DeFalco Thomas A.、Petriello Annalise、Font Farre Maria、Schwessinger Benjamin、Ntoukakis Vardis、Stransfeld Lena、Jones Alexandra M. E.、Menke Frank L. H.、Zipfel Cyril
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 118 Issue: 38

    • DOI

      10.1073/pnas.2108242118

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ネコブセンチュウエフェクターによる植物免疫抑制機構の解析2021

    • Author(s)
      佐藤 一輝、門田 康弘、Gan Pamela、植原 健人、槇 紀子、Mukhtar Shahid M.、白須 賢
    • Organizer
      令和4年度日本植物病理学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Transcriptomic analysis of resistant and susceptible responses in a new model root-knot nematode infection system using Solanum torvum and Meloidogyne arenaria2021

    • Author(s)
      Sato Kazuki、Uehara Taketo、Holbein Julia、Sasaki-Sekimoto Yuko、Gan Pamela、Bino Takahiro、Yamaguchi Katsushi、Ichihashi Yasunori、Maki Noriko、Shigenobu Shuji、Ohta Hiroyuki、Franke Rochus B.、Siddique Shahid、Grundler Florian M. W.、Suzuki Takamasa、Kadota Yasuhiro、Shirasu Ken
    • Organizer
      2021 IS-MPMI Congress: eSymposia Series
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規モデル感染システムを用いた線虫抵抗性・感受性応答のトランスクリプトーム解析2021

    • Author(s)
      佐藤 一輝、植原 健人、Gan Pamela、槇 紀子、鈴木 孝征、門田 康弘、白須 賢
    • Organizer
      日本線虫学会 第28回大会
    • Related Report
      2020 Annual Research Report
  • [Remarks] 理化学研究所 環境資源科学研究センター 植物免疫研究グループ

    • URL

      http://plantimmunity.riken.jp/

    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2025-03-27  

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