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Identification, functional analysis and application of host plant factors involved in susceptibility

Research Project

Project/Area Number 20H02995
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Asai Shuta  国立研究開発法人理化学研究所, 環境資源科学研究センター, 上級研究員 (30723580)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2020: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords罹病性遺伝子 / 植物病原菌間相互作用 / 病害防除
Outline of Research at the Start

生産現場では、抵抗性遺伝子による抵抗性品種が利用されてきたが、新レースの出現と抵抗性打破のリスクを常に招いており、新たな遺伝資源を利用した新奇病害抵抗性品種の育成が強く望まれている。宿主植物と病原菌の関係においては、病原菌が標的とする、感染するために必要な宿主側の遺伝子(罹病性遺伝子)が存在し、罹病性遺伝子を欠損させた植物体には病原菌は感染することができない。罹病性遺伝子は、病原菌にとって宿主侵入前後における必須因子であることが予想されるため、持続的な病害抵抗性に貢献しうる遺伝資源と期待される。そこで本研究では、新奇罹病性遺伝子を同定し、その機能を解析すると共に、有用作物への応用を目指す。

Outline of Final Research Achievements

In the relationship between host plants and pathogens, there are genes on the host side, so-called susceptibility genes, that are required for infection by pathogens. Mutation or loss of a susceptibility gene can limit the ability of the pathogen to cause disease. In this study, I obtained susceptibility gene candidates through forward genetic approach and cell-specific transcriptome approach using Arabidopsis thaliana, a model plant. I am proceeding with their functional analysis and aiming to apply them to crops.

Academic Significance and Societal Importance of the Research Achievements

病害防除のため、生産現場では古くから抵抗性品種が利用されてきた。しかし、これら抵抗性品種の多くは、gene-for-geneで制御されるような抵抗性遺伝子による抵抗性であり、新レースの出現と抵抗性打破のリスクを常に招いている。罹病性遺伝子は、病原菌にとって宿主侵入前後における必須因子であることが予想されるため、持続的な病害抵抗性に貢献しうる遺伝資源と期待される。また、罹病性遺伝子は劣性遺伝子であるため、有用作物において遺伝子組換え以外の手法で遺伝子破壊をすることで、耐病性を付与することが可能である。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (22 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (4 results) Journal Article (9 results) (of which Int'l Joint Research: 4 results,  Open Access: 7 results,  Peer Reviewed: 8 results) Presentation (6 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Int'l Joint Research] センズベリー研究所/バース大学(英国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] アムステルダム大学(オランダ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] デューク大学(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] アムステルダム大学(オランダ)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Inducible TRAP RNA profiling reveals host genes expressed in Arabidopsis cells haustoriated by downy mildew2023

    • Author(s)
      Asai S., Cevik V., Jones D.G.J. and Shirasu K.
    • Journal Title

      bioRxiv

    • DOI

      10.1101/2023.01.17.524393

    • Related Report
      2022 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Long-read direct RNA sequencing reveals epigenetic regulation of chimeric gene-transposon transcripts in Arabidopsis thaliana2022

    • Author(s)
      Berthelier Jeremy、Furci Leonardo、Asai Shuta、Sadykova Munissa、Shimazaki Tomoe、Shirasu Ken、Saze Hidetoshi
    • Journal Title

      biorxiv

      Volume: -

    • DOI

      10.1101/2022.09.21.507229

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recognition of pathogen-derived sphingolipids in <i>Arabidopsis</i>2022

    • Author(s)
      Kato H.、Nemoto K.、Shimizu M.、Abe A.、Asai S.、Ishihama N.、Matsuoka S.、Daimon T.、Ojika M.、Kawakita K.、Onai K.、Shirasu K.、Yoshida M.、Ishiura M.、Takemoto D.、Takano Y.、Terauchi R.
    • Journal Title

      Science

      Volume: 376 Issue: 6595 Pages: 857-860

    • DOI

      10.1126/science.abn0650

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A pair of effectors encoded on a conditionally dispensable chromosome of Fusarium oxysporum suppress host-specific immunity2021

    • Author(s)
      Ayukawa Yu、Asai Shuta、Gan Pamela、Tsushima Ayako、Ichihashi Yasunori、Shibata Arisa、Komatsu Ken、Houterman Petra M.、Rep Martijn、Shirasu Ken、Arie Tsutomu
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1 Pages: 707-707

    • DOI

      10.1038/s42003-021-02245-4

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Draft genome resources for Brassicaceae pathogens Fusarium oxysporum f. sp. raphani and Fusarium oxysporum f. sp. rapae.2021

    • Author(s)
      Asai Shuta、Ayukawa Yu、Gan Pamela、Shirasu Ken
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 34 Issue: 11 Pages: 1316-1319

    • DOI

      10.1094/mpmi-06-21-0148-a

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oxicam-type non-steroidal anti-inflammatory drugs inhibit NPR1-mediated salicylic acid pathway2021

    • Author(s)
      Ishihama Nobuaki、Choi Seung-won、Noutoshi Yoshiteru、Saska Ivana、Asai Shuta、Takizawa Kaori、He Sheng Yang、Osada Hiroyuki、Shirasu Ken
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 7303-7303

    • DOI

      10.1038/s41467-021-27489-w

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Complete Genome Sequence of Pseudomonas amygdali pv. tabaci Strain 6605, a Causal Agent of Tobacco Wildfire Disease2021

    • Author(s)
      Matsui Hidenori、Nishimura Takafumi、Asai Shuta、Masuda Sachiko、Shirasu Ken、Yamamoto Mikihiro、Noutoshi Yoshiteru、Toyoda Kazuhiro、Ichinose Yuki
    • Journal Title

      Microbiology Resource Announcements

      Volume: 10 Issue: 28

    • DOI

      10.1128/mra.00405-21

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Studies on infection mechanisms of oomycete plant pathogens2020

    • Author(s)
      ASAI S.
    • Journal Title

      Japanese Journal of Phytopathology

      Volume: 86 Issue: 4 Pages: 265

    • DOI

      10.3186/jjphytopath.86.265

    • NAID

      130007956895

    • ISSN
      0031-9473, 1882-0484
    • Year and Date
      2020-11-25
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Studies on infection mechanisms of oomycete plant pathogens2020

    • Author(s)
      Asai Shuta
    • Journal Title

      Journal of General Plant Pathology

      Volume: 86 Issue: 6 Pages: 526-527

    • DOI

      10.1007/s10327-020-00959-7

    • NAID

      130007956895

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] ダイコン萎黄病菌の病原性に関与するペアエフェクターの同定2023

    • Author(s)
      浅井秀太
    • Organizer
      令和4年度植物病理学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ゲノムから迫るフザリウムの病原性2022

    • Author(s)
      浅井秀太
    • Organizer
      第21回植物病原菌類談話会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] ダイコン萎黄病菌の病原性染色体の同定2022

    • Author(s)
      浅井秀太
    • Organizer
      令和3年度植物病理学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] A pair of effectors encoded on a conditionally dispensable chromosome of Fusarium oxysporum f. sp. conglutinans suppress Arabidopsis-specific immunity2021

    • Author(s)
      浅井秀太
    • Organizer
      The International Society for Molecular Plant Microbe Interactions (IS-MPMI) Congress: eSymposia
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A pair of effectors involving in suppression of Arabidopsis-specific immunity are conserved in Arabidopsis-infecting Fusarium oxysporum2021

    • Author(s)
      浅井秀太
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ホウレンソウ萎凋病菌の病原性染色体の同定2021

    • Author(s)
      浅井秀太
    • Organizer
      令和3年度植物病理学会大会
    • Related Report
      2020 Annual Research Report
  • [Remarks] 理化学研究所植物免疫研究グループ

    • URL

      http://plantimmunity.riken.jp/index_ja.html

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report 2020 Annual Research Report
  • [Patent(Industrial Property Rights)] 病原ゲノミクスによるフザリウム菌土壌診断法2021

    • Inventor(s)
      浅井秀太、ガンパメラ、増田幸子、鮎川侑、白須賢
    • Industrial Property Rights Holder
      浅井秀太、ガンパメラ、増田幸子、鮎川侑、白須賢
    • Industrial Property Rights Type
      特許
    • Filing Date
      2021
    • Related Report
      2021 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 病原ゲノミクスによるフザリウム菌土壌診断法2020

    • Inventor(s)
      浅井秀太、ガンパメラ、増田幸子、鮎川侑、白須賢
    • Industrial Property Rights Holder
      浅井秀太、ガンパメラ、増田幸子、鮎川侑、白須賢
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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