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Elucidation of the molecular mechanism of juvenile hormone biosynthetic pathway by analysis of a novel corpora allata-specific transcription factor

Research Project

Project/Area Number 20H03004
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39050:Insect science-related
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Kayukawa Takumi  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (70580463)

Co-Investigator(Kenkyū-buntansha) 松尾 隆嗣  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (70301223)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2022: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2021: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2020: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords幼若ホルモン / アラタ体 / 生合成経路 / 転写因子 / 生合成酵素
Outline of Research at the Start

幼若ホルモン(JH)は,変態抑制作用を有する昆虫固有のホルモンであるが,その生合成制御機構に関しては未だ不明な点が多い.これまで我々は,JH生合成器官であるアラタ体で特異的に発現し,JH生合成酵素遺伝子を制御する新規転写因子(corpora allata-specific transcriptional factor, CAsp-TF)の単離に成功している.本研究では,様々な昆虫の優れた解析ツールを駆使してCAsp-TFの機能解析を行うと共に,未知JH生合成酵素遺伝子の単離・機能解析を行うことで,JH生合成の分子制御機構を解明する.

Outline of Final Research Achievements

The metamorphosis-suppressing action of juvenile hormone (JH) is a universal and characteristic physiological action in insects and is one of the factors that have contributed greatly to their prosperity. Therefore, JH is attracting attention not only for its academic interest but also as a promising target for pesticide discovery. Recently, JH signaling pathways in target cells have been elucidated, but the regulatory mechanism of JH biosynthesis in the corpora allata (CA), the biosynthetic organ of JH, remains unclear. In this study, we identified a transcription factor (dead ringer, Dri) that is expressed specifically in the CA and elucidated a novel molecular mechanism of JH biosynthesis by Dri.

Academic Significance and Societal Importance of the Research Achievements

JHは脱皮・変態の他に,休眠・卵成熟・相変異・カースト分化等の昆虫の生理現象に関わることから,DriによるJH生合成の分子制御機構は,JHが関わる多岐の生理現象の解明に派生すると考えられる.また,Driについてさらに詳しく解析を進めることで,将来的に薬剤や遺伝子工学技術を使って,害虫や益虫の発育をコントロールする技術開発に期待できる.

Report

(2 results)
  • 2022 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • Research Products

    (13 results)

All 2021 2020 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Identification of novel juvenile-hormone signaling activators <i>via</i> high-throughput screening with a chemical library2021

    • Author(s)
      Takumi Kayukawa, Kenjiro Furuta, Kiyoaki Yonesu, Takayoshi Okabe
    • Journal Title

      Journal of Pesticide Science

      Volume: 46 Issue: 1 Pages: 53-59

    • DOI

      10.1584/jpestics.D20-070

    • NAID

      130007992087

    • ISSN
      1348-589X, 1349-0923
    • Year and Date
      2021-02-20
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Elucidation of molecular mechanism underlying juvenile hormone action and development of molecular-targeted insecticide2020

    • Author(s)
      粥川琢巳
    • Journal Title

      Japanese Journal of Pesticide Science

      Volume: 45 Issue: 2 Pages: 97-103

    • DOI

      10.1584/jpestics.W20-36

    • NAID

      130007988761

    • ISSN
      2187-0365, 2187-8692
    • Year and Date
      2020-08-20
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a juvenile-hormone signaling inhibitor via high-throughput screening of a chemical library2020

    • Author(s)
      Kayukawa Takumi, Furuta Kenjiro, Nagamine Keisuke, Shinoda Tetsuro, Yonesu Kiyoaki, Okabe Takayoshi
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 18413-18413

    • DOI

      10.1038/s41598-020-75386-x

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment and characterization of novel cell lines derived from six lepidopteran insects collected in the2020

    • Author(s)
      Kazuyo Watanabe, Isao Kobayashi, Masatsugu Hatakeyama, Takumi Kayukawa, Gaku Akiduki
    • Journal Title

      In Vitro Cellular & Developmental Biology - Animal

      Volume: 56 Issue: 6 Pages: 425-429

    • DOI

      10.1007/s11626-020-00438-5

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] 培養細胞系を用いた大規模スクリーニングから得られた新規幼若ホルモンアゴニスト2021

    • Author(s)
      粥川 琢巳、古田 賢次郎、米須清明、岡部隆義
    • Organizer
      第65回日本応用動物昆虫学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] マツノマダラカミキリ由来培養細胞の樹立2021

    • Author(s)
      渡邊和代、高務淳、相川拓也、粥川琢巳
    • Organizer
      第65回日本応用動物昆虫学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 幼若ホルモンアンタゴニストNY52 誘導体の合成と薬理学的特性2021

    • Author(s)
      古田賢次郎、山田直子、粥川琢巳
    • Organizer
      日本農薬学会第46回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Establishment of a simple method for ex vivo honey bee cell culture capable of gene expression analysis2021

    • Author(s)
      Kazuyo Watanabe, Mikio Yoshiyama, Gaku Akiduki, Kakeru Yokoi, Hiroko Hoshida, Takumi Kayukawa, Mari Ogihara, Kiyoshi Kimura, Masatsugu Hatakeyama
    • Organizer
      Coloss Asia e-meeting 2021
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 幼若ホルモンの働きを抑える薬剤探索法の開発と新たな昆虫成長制御剤の候補となる化合物の発見

    • URL

      https://www.naro.go.jp/publicity_report/press/laboratory/nias/137143.html

    • Related Report
      2020 Annual Research Report
  • [Remarks] 大規模スクリーニングから得られた新規幼若ホルモン活性化合物

    • URL

      https://www.naro.go.jp/project/results/4th_laboratory/nias/2020/nias20_s22.html

    • Related Report
      2020 Annual Research Report
  • [Remarks] 新規チョウ目昆虫培養細胞7種の樹立とその配布

    • URL

      https://www.naro.go.jp/project/results/4th_laboratory/nias/2020/nias20_s01.html

    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] 大規模スクリーニングから得られた幼若ホルモン活性を有する有害生物防除剤2021

    • Inventor(s)
      粥川琢巳、古田賢次郎
    • Industrial Property Rights Holder
      粥川琢巳、古田賢次郎
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-007093
    • Filing Date
      2021
    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] サバクトビバッタの細胞株の製造方法2021

    • Inventor(s)
      粥川琢巳、渡邊和代
    • Industrial Property Rights Holder
      粥川琢巳、渡邊和代
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-011741
    • Filing Date
      2021
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2025-03-27  

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