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Estimating the absolute number of individuals of Lates japonicus using the CKMR method

Research Project

Project/Area Number 20H03012
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39060:Conservation of biological resources-related
Research InstitutionFisheries Research and Education Agency

Principal Investigator

Takahashi Hiroshi  国立研究開発法人水産研究・教育機構, 水産大学校, 准教授 (90399650)

Co-Investigator(Kenkyū-buntansha) 中山 耕至  京都大学, 農学研究科, 助教 (50324661)
橋口 康之  大阪医科薬科大学, 医学部, 講師 (70436517)
秋田 鉄也  国立研究開発法人水産研究・教育機構, 水産資源研究所(横浜), 主任研究員 (60625507)
武島 弘彦  福井県立大学, 海洋生物資源学部, 客員研究員 (50573086)
小関 右介  大妻女子大学, 家政学部, 准教授 (00513772)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2022: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2020: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords遺伝的多様性 / 個体数推定
Outline of Research at the Start

本研究は,脊椎動物の中で最も遺伝的多様性が低く,生活史の多くが不明である“幻の魚”アカメについて,遺伝資源の保全・管理の根幹をなすパラメータである現在の有効集団サイズ(Ne)を明らかすることを目的とする。そのために,複数年級群からサンプリングされた当歳魚の血縁関係に基づく新たな遺伝的標識再捕法の理論を構築し,それに基づいてアカメが現在何個体いるのかを推定する。得られた結果に基づき,定量的採集が困難な海洋生物の保全に新たな道筋を示すとともに,国と県で異なる本種の保全策の統一に役立つ情報を得る。

Outline of Final Research Achievements

This study aimed to determine the current effective population size (Ne), a parameter fundamental to the conservation and management of genetic resources in Lates japonicus, which has the lowest genetic diversity among vertebrates and much of its life history is unknown. To this end, we developed a new theory of close-kin mark-recapture (CKMR) based on kinship relationships of yearlings sampled from multiple year classes, and estimated the current Ne based on this theory. The number of half-sibling pairs between year classes was estimated from whole genome sequencing data and Ne estimation was performed by CKMR, resulting in an estimated range of 489-1263 adult fish during the study time. In the process, ecological information necessary to improve the accuracy of the estimation was also identified.

Academic Significance and Societal Importance of the Research Achievements

種の個体数は遺伝資源の保全・管理に必須のパラメータであるにもかかわらず,多くの種,特に成体の定量的採集の困難な海洋生物においては,その推定は極めて困難である。本研究は,脊椎動物中最も遺伝的多様性が低く,“幻の魚”と呼ばれる大型魚アカメについて,成魚の採集によらない,遺伝標識再捕(CKMR)法に基づく個体数推定を行い,研究期間中の成魚個体数を一定の精度で明らかにした。本研究で実践された個体数推定法およびそのための理論構築は,定量的採集が困難な海洋生物の保全・管理に新たな道筋を示すとともに,アカメの極めて低い遺伝的多様性の謎を解明し,国と県で統一されていない保全策にも一石を投ずると期待される。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (35 results)

All 2023 2022 2021 2020

All Journal Article (18 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 18 results,  Open Access: 6 results) Presentation (16 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Genotyping of two congeneric bitterling fish species by nuclear SNP markers and the detection of hybridization in a sympatric region2023

    • Author(s)
      Hata Hiroki、Taniguchi Rintaro、Yamashita Naoki、Hashiguchi Yasuyuki、Nakajima Jun、Takeyama Tomohiro
    • Journal Title

      Ecological Research

      Volume: - Issue: 4 Pages: 571-582

    • DOI

      10.1111/1440-1703.12387

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The detailed population genetic structure of the rare endangered latid fish akame Lates japonicus with extremely low genetic diversity revealed from single-nucleotide polymorphisms2023

    • Author(s)
      Naito Takuya、Nakayama Kouji、Takeshima Hirohiko、Hashiguchi Yasuyuki、Akita Tetsuya、Yamasaki Yo Y.、Mishina Tappei、Takeshita Naohiko、Nagano Atsushi J.、Takahashi Hiroshi
    • Journal Title

      Conservation Genetics

      Volume: online Issue: 4 Pages: 1-13

    • DOI

      10.1007/s10592-023-01517-2

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recent reduction of genetic diversity in markless form of the red-spotted masu salmon Oncorhynchus masou ishikawae in the Ono River, Kyushu, Japan2023

    • Author(s)
      Kitanishi Shigeru、Kondou Takuya、Takahashi Hiroshi、Takeshita Naohiko、Nagata Shingo、Edo Kaneaki
    • Journal Title

      Conservation Genetics

      Volume: - Issue: 4 Pages: 489-499

    • DOI

      10.1007/s10592-023-01515-4

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of the noncoding features of sea snake mitochondrial genomes within Elapidae2022

    • Author(s)
      Xiaokaiti Xiakena、Hashiguchi Yasuyuki、Ota Hidetoshi、Kumazawa Yoshinori
    • Journal Title

      Genes

      Volume: 13 Issue: 8 Pages: 1470-1470

    • DOI

      10.3390/genes13081470

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ancient Admixture in Freshwater Halfbeaks of the Genus Nomorhamphus in Southeast Sulawesi2022

    • Author(s)
      Nofrianto Andy B.、Lawelle Sjamsu A.、Mokodongan Daniel F.、Masengi Kawilarang W. A.、Inomata Nobuyuki、Hashiguchi Yasuyuki、Kitano Jun、Sumarto Bayu K. A.、Kakioka Ryo、Yamahira Kazunori
    • Journal Title

      Zoological Science

      Volume: 39 Issue: 5 Pages: 453-458

    • DOI

      10.2108/zs220023

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Estimating contemporary migration numbers of adults based on kinship relationships in iteroparous species2022

    • Author(s)
      Akita Tetsuya
    • Journal Title

      Molecular Ecology Resources

      Volume: 22 Issue: 8 Pages: 3006-3017

    • DOI

      10.1111/1755-0998.13682

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Local Adaptation and the Evolution of Genome Architecture in Threespine Stickleback2022

    • Author(s)
      Qiushi Li、Dorothea Lindtke、Carlos Rodriguez-Ramirez、Ryo Kakioka、Hiroshi Takahashi、Atsushi Toyoda、Jun Kitano、Rachel L. Ehrlich、Joshua Chang Mell、Sam Yeaman
    • Journal Title

      Genome Biology and Evolution

      Volume: 14(6) Issue: 6

    • DOI

      10.1093/gbe/evac075

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of salinity on nest building behaviour in the nine‐spined stickleback <i>Pungitius sinensis</i>2022

    • Author(s)
      Machida Yoshiyasu、Takahashi Hiroshi、Tsuruta Tetsuya、Goto Akira
    • Journal Title

      Journal of Fish Biology

      Volume: 102 Issue: 2 Pages: 516-519

    • DOI

      10.1111/jfb.15264

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 水温と塩分がアカメ未成魚の摂餌と成長に及ぼす影響2021

    • Author(s)
      竹下直彦・近藤卓哉・池田 至・高橋 洋・永田新悟・星野和夫
    • Journal Title

      水産大学校研究報告

      Volume: 70 Pages: 27-34

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interploidy gene flow involving the sexual-asexual cycle facilitates the diversification of gynogenetic triploid Carassius fish2021

    • Author(s)
      Mishina Tappei、Takeshima Hirohiko、Takada Mikumi、Iguchi Kei’ichiro、Zhang Chunguang、Zhao Yahui、Kawahara-Miki Ryouka、Hashiguchi Yasuyuki、Tabata Ryoichi、Sasaki Takeshi、Nishida Mutsumi、Watanabe Katsutoshi
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 22485-22485

    • DOI

      10.1038/s41598-021-01754-w

    • NAID

      120007169935

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fine‐scale phylogeography of coastal fishes in the South China Sea: Possible roles of biological traits and geography2021

    • Author(s)
      Muto Nozomu、Kakioka Ryo、Ghaffar Mazlan Abd、Quan Nguyen Van、Vilasri Veera、Traifalgar Rex F. M.、Babaran Ricardo P.、Muto Fumihito、Takeshima Hirohiko、Motomura Hiroyuki、Ishikawa Satoshi
    • Journal Title

      Journal of Biogeography

      Volume: 48 Issue: 11 Pages: 2785-2800

    • DOI

      10.1111/jbi.14239

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Difficulty in sex identification of two local populations of red-spotted masu salmon using two salmonid male-specific molecular markers2021

    • Author(s)
      Ueda Rui、Takeshima Hirohiko、Sato Takuya
    • Journal Title

      Ichthyological Research

      Volume: 69 Issue: 2 Pages: 275-279

    • DOI

      10.1007/s10228-021-00837-y

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] First report of natural hybridization between two yellowtails, Seriola quinqueradiata and S. lalandi.2021

    • Author(s)
      Hiroshi Takahashi, Taiki Kurogoushi, Ryo Shimoyama, Hiroyuki Yoshikawa
    • Journal Title

      Ichthyological Research

      Volume: 68 Issue: 1 Pages: 139-144

    • DOI

      10.1007/s10228-020-00752-8

    • NAID

      40022462106

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nearly unbiased estimator of contemporary Ne/N based on kinship relationships2020

    • Author(s)
      Akita Tetsuya
    • Journal Title

      Ecology and Evolution

      Volume: 10 Issue: 19 Pages: 10343-10352

    • DOI

      10.1002/ece3.6421

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vertical distribution of larval Pacific bluefin tuna, Thunnus orientalis, in the Japan Sea2020

    • Author(s)
      Kodama Taketoshi、Ohshimo Seiji、Tawa Atsushi、Furukawa Seishiro、Nohara Kenji、Takeshima Hirohiko、Chiba Satoru N.、Ishihara Taiki、Sawai Etsuro、Kawazu Masanori、Okazaki Makoto、Ono Tsuneo、Suzuki Nobuaki
    • Journal Title

      Deep Sea Research Part II: Topical Studies in Oceanography

      Volume: 175 Pages: 104785-104785

    • DOI

      10.1016/j.dsr2.2020.104785

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ecosystem size predicts the probability of speciation in migratory freshwater fish2020

    • Author(s)
      Yamasaki Yo Y.、Takeshima Hirohiko、Kano Yuichi、Oseko Naoharu、Suzuki Toshiyuki、Nishida Mutsumi、Watanabe Katsutoshi
    • Journal Title

      Molecular Ecology

      Volume: - Issue: 16 Pages: 3071-3083

    • DOI

      10.1111/mec.15415

    • NAID

      120006843524

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The distribution and early growth of juvenile Pacific bluefin tuna Thunnus orientalis around Sado Island in the eastern Sea of Japan2020

    • Author(s)
      Tanaka Hiroshige、Kodama Taketoshi、Suzuki Nobuaki、Mochizuki Yosuke、Ashida Hiroshi、Sato Takuya、Takeshima Hirohiko、Nohara Kenji
    • Journal Title

      Fisheries Science

      Volume: 86 Issue: 6 Pages: 1019-1028

    • DOI

      10.1007/s12562-020-01470-w

    • NAID

      40022414247

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide patterns of divergence and introgression after secondary contact between Pungitius sticklebacks2020

    • Author(s)
      Yamasaki Yo Y.、Kakioka Ryo、Takahashi Hiroshi、Toyoda Atsushi、Nagano Atsushi J.、Machida Yoshiyasu、M?ller Peter R.、Kitano Jun
    • Journal Title

      Philosophical Transactions of the Royal Society B: Biological Sciences

      Volume: 375 Issue: 1806 Pages: 20190548-20190548

    • DOI

      10.1098/rstb.2019.0548

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 遺伝的多様性の評価・モニタリングの展開と課題-ポスト2020生物多様性枠組に向けて2023

    • Author(s)
      秋田鉄也
    • Organizer
      第70回日本生態学会大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 遺伝的要因によると思われるヒラメ稚魚の無眼側黒化2023

    • Author(s)
      後藤千佳・中山耕至・加藤文仁・村田照之・田川正朋
    • Organizer
      令和5年度日本水産学会春期大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ゼニタナゴのドラフトゲノム決定及び食性関連遺伝子群の解析2022

    • Author(s)
      橋口康之・斉藤憲治
    • Organizer
      2022年度日本魚類学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 家系生態学:ゲノム情報から近年の生態情報を抽出して希少種保全に貢献する2022

    • Author(s)
      秋田鉄也
    • Organizer
      令和4年度 水産育種研究会 定例シンポジウム
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Determinism and contingency in the genetic basis of parallel armor plate evolution of sticklebacks2022

    • Author(s)
      Yo Yamasaki, Tomoyuki Kokita, Koki Kato, Asato Irifune, Mitsuki Kuroda, Hiroshi Takahashi, Yoshiyasu Machida, Asano Ishikawa, Mikiko Tanaka, Seiichi Mori, Atsushi Toyoda, Jun Kitano
    • Organizer
      Congress of the European Society for Evolutionary Biology
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] キタノトミヨにおける卵サイズの遺伝基盤の解明2022

    • Author(s)
      梁田椋也・楠永 楓・高橋 洋
    • Organizer
      2022年度日本魚類学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 日本産トラフグ属魚類全11種(Takifugu spp.)の種・雑種判別マーカーの開発2022

    • Author(s)
      高橋 洋・柿岡 諒
    • Organizer
      2022年度日本魚類学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 森と川のつながりが維持するアマゴの生活史多様性:野外操作実験とゲノム解析の融合へ2022

    • Author(s)
      上田るい, 金岩稔, 照井慧, 瀧本 岳, 武島弘彦, 勝村啓史, 橋口康之, 山﨑 曜, 佐藤拓哉
    • Organizer
      第69回日本生態学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] メバル属魚類の接合後隔離と形態分化にかかわる遺伝領域の探索2021

    • Author(s)
      武藤望生・柿岡 諒・永野 惇・坪井健人・清水祐大・猪瀬周・清水洋平・川崎琢真・高橋 洋
    • Organizer
      日本魚類学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] The genetic basis of egg size variation in ninespine stickleback, Pungitius pungitius2021

    • Author(s)
      Ryoya Yanada, Kaede Kusunaga, Hiroshi Takahashi
    • Organizer
      26th Joint International Symposium between National Fisheries University and Pukyong National University
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 硬骨魚類における嗅覚受容体遺伝子の多様性:生活史との関連に着目して2021

    • Author(s)
      橋口康之
    • Organizer
      日本進化学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 魚類におけるフェロモン受容体OR114の起源と分子進化2021

    • Author(s)
      橋口康之・井上順治・中島 淳
    • Organizer
      日本魚類学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 近親標識法による集団間の移動個体数推定2021

    • Author(s)
      秋田鉄也
    • Organizer
      第37回個体群生態学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Multiple paths to the same goal: the repeated use of hotspot genes for convergent plate reduction in sticklebacks2021

    • Author(s)
      Yo Yamasaki, Tomoyuki Kokita, Koki Kato, Asato Irifune, Mitsuki Kuroda, Hiroshi Takahashi, Yoshiyasu Machida, Asano Ishikawa, Mikiko Tanaka, Seiichi Mori, Atsushi Toyoda, Jun Kitano
    • Organizer
      The 68th Annual Meeting of the Ecological Society of Japan (ESJ67)
    • Related Report
      2020 Annual Research Report
  • [Presentation] AFLP法による種同定に基づく九州北部海域のメバル複合種群の計数形質2021

    • Author(s)
      安武由矢・高橋 洋・望岡典隆
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Molecular diagnostic methods for identifying pufferfish species and their hybrid to prevent pufferfish poisoning2020

    • Author(s)
      Hiroshi Takahashi
    • Organizer
      The 35th Annual Meeting of the Korean Society of Food Hygiene and Safety
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] Fish Diversity of Japan: Evolution, Zoogeography, and Conservation2022

    • Author(s)
      Hiroshi Takahashi他著,Yoshiaki Kai他編
    • Total Pages
      454
    • Publisher
      Springer
    • ISBN
      9789811674273
    • Related Report
      2021 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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