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Comprehending the range of the mangrove rhizosphere - An investigation of plants effects on soil nitrogen dynamics at high spatial resolution

Research Project

Project/Area Number 20H03029
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40010:Forest science-related
Research InstitutionKyoto University

Principal Investigator

Koyama Lina  京都大学, 情報学研究科, 准教授 (50378832)

Co-Investigator(Kenkyū-buntansha) 井上 智美  国立研究開発法人国立環境研究所, 生物多様性領域, 主幹研究員 (80435578)
黒岩 恵  東京農工大学, 工学(系)研究科(研究院), 助教 (00761024)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2022: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2021: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2020: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywordsマングローブ / 土壌 / 窒素循環 / 硝化 / 土壌微生物 / 硝酸態窒素 / 微小透析法 / 嫌気性土壌 / 脱窒 / 根圏 / 窒素 / アンモニア酸化細菌 / アンモニア酸化古細菌
Outline of Research at the Start

マングローブ林は潮汐による冠水の影響を受け、土壌は低酸素状態であることが多く、窒素養分条件もその影響を受ける。これまでの調査で、マングローブ植物と根圏の土壌の間には酸素と窒素を供給し合う共生的関係が存在することが示唆された。本研究では、空間的に高い解像度での調査を可能にする微小透析法を用いて土壌中の窒素養分の分布を調べ、マングローブ植物と土壌微生物の共生的な関係が、空間的・時間的にどこまで広がっており、マングローブ生態系の物質循環における寄与がどの程度なのか?という問いに答えることを目指す。

Outline of Final Research Achievements

Mangrove forests are established in environments where the soil is regularly flooded by tides in tropical and subtropical coastal areas. It is a harsh environment for plant growth due to soil hypoxia and nutrient run-off. Plants generally use ammonium and nitrate in the soil as a N source, but in mangrove forests, where the soil is often anaerobic, nitrate, which requires oxygen for production, has not been considered important. In this study, a root-box experiment was conducted with a mangrove species, showing that plants supply oxygen to the soil, which is used by soil microbes to produce nitrate that can serve as a N source for plants, and that such plant-microbe relationships occur on a small spatial scale of less than a few cm.

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的意義は、マングローブ林における窒素循環の中で、貧酸素土壌条件により重視されてこなかった硝酸態窒素を介した経路において、植物による土壌微生物への酸素供給という面からの植物の寄与を明らかにし、その空間スケールを示したことにあると考える。また、土壌養分条件の調査手法としてこれまでに国内で適用例のなかった微小透析法について検証を行い、土壌タイプが結果に与える影響を示した。その結果、一定の条件下では微小透析法の結果は従来手法による調査結果との直接の対応関係を示すことが明らかとなった。

Report

(4 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (26 results)

All 2024 2023 2022 2021 2020

All Journal Article (15 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 15 results,  Open Access: 9 results) Presentation (10 results) (of which Int'l Joint Research: 2 results) Book (1 results)

  • [Journal Article] Citizen‐participatory nationwide survey of mountain streamwater chemistry in Japan in 2022: Comparison of nitrate concentrations with the 2003 survey2024

    • Author(s)
      Makino Soyoka、Tokuchi Naoko、Akaishi Daisuke、Koyama Lina A.、Komai Yukio、Itaya Yoshimi、Maung Nay Lin、Ishimaru Kanae、Oda Tomoki、Shibata Rei、Numamoto Shinya
    • Journal Title

      Ecological Research

      Volume: - Issue: 3 Pages: 277-292

    • DOI

      10.1111/1440-1703.12465

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Bruguiera gymnorhiza forms mycorrhizal associations but Rhizophora stylosa does not: A pot experiment using mangrove soil and Rhizophoraceae seedlings2024

    • Author(s)
      Akaji Yasuaki、Inoue Tomomi、Kohzu Ayato、Taniguchi Takeshi、Baba Shigeyuki
    • Journal Title

      Aquatic Botany

      Volume: 192 Pages: 103748-103748

    • DOI

      10.1016/j.aquabot.2023.103748

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quest for Nitrous Oxide-reducing Bacteria Present in an Anammox Biofilm Fed with Nitrous Oxide2024

    • Author(s)
      Oba Kohei、Suenaga Toshikazu、Yasuda Shohei、Kuroiwa Megumi、Hori Tomoyuki、Lackner Susanne、Terada Akihiko
    • Journal Title

      Microbes and Environments

      Volume: 39 Issue: 1 Pages: n/a

    • DOI

      10.1264/jsme2.ME23106

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diazotrophic nitrogen fixation through aerial roots occurs in <i>Avicennia marina</i>: implications for adaptation of mangrove plant growth to low‐nitrogen tidal flats2023

    • Author(s)
      Inoue Tomomi、Kohzu Ayato、Akaji Yasuaki、Miura Shingo、Baba Shigeyuki
    • Journal Title

      New Phytologist

      Volume: 241 Issue: 4 Pages: 1464-1475

    • DOI

      10.1111/nph.19442

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of soil temperature on the morphological and physiological traits of Kandelia obovata seedlings2023

    • Author(s)
      Akaji Yasuaki、Inoue Tomomi
    • Journal Title

      Plant Ecology

      Volume: 224 Issue: 6 Pages: 579-589

    • DOI

      10.1007/s11258-023-01325-1

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature dependence of <scp> O <sub>2</sub> </scp> respiration in mangrove leaves and roots: implications for seedling dispersal phenology2022

    • Author(s)
      Inoue Tomomi、Akaji Yasuaki、Baba Shigeyuki、Noguchi Ko
    • Journal Title

      New Phytologist

      Volume: 237 Issue: 1 Pages: 100-112

    • DOI

      10.1111/nph.18513

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Relationship between plant growth and soil chemical properties in a mangrove afforestation stand, Kiribati2022

    • Author(s)
      Inoue Tomomi、Akaji Yasuaki、Kohzu Ayato、Hinokidani Ko、Adachi Hiroshi、Kezuka Mio、Baba Shigeyuki
    • Journal Title

      Plant and Soil

      Volume: 479 Issue: 1-2 Pages: 559-571

    • DOI

      10.1007/s11104-022-05545-8

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Exploring the functions of efficient canonical denitrifying bacteria as N2O sinks: Implications from 15N tracer and transcriptome analyses2022

    • Author(s)
      Kohei Oba, Toshikazu Suenaga, Megumi Kuroiwa, Shohei Riya, Akihiko Terada
    • Journal Title

      Environmental Science & Technology

      Volume: 56 Issue: 16 Pages: 11694-11706

    • DOI

      10.1021/acs.est.2c02119

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alteration of dissimilatory nitrate reduction pathways in the intertidal sediment during macroalgae blooms2022

    • Author(s)
      Senga Y, Sato T, Shibaki K, Kuroiwa M, Nohara S, Suwa Y
    • Journal Title

      Water

      Volume: 14 Issue: 19 Pages: 3022-3022

    • DOI

      10.3390/w14193022

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitrous oxide-sink capability of denitrifying bacteria impacted by nitrite and pH2022

    • Author(s)
      Zhou Yiwen、Zhao Shuting、Suenaga Toshikazu、Kuroiwa Megumi、Riya Shohei、Terada Akihiko
    • Journal Title

      Chemical Engineering Journal

      Volume: 428 Pages: 132402-132402

    • DOI

      10.1016/j.cej.2021.132402

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal changes in nitrate assimilation of boreal woody species: importance of the leaf-expansion period2022

    • Author(s)
      Koyama Lina A.、Kielland Knut
    • Journal Title

      Trees

      Volume: - Issue: 3 Pages: 941-951

    • DOI

      10.1007/s00468-021-02259-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Growth temperature affects O2 consumption rates and plasticity of respiratory flux to support shoot growth at various growth temperatures2021

    • Author(s)
      Inoue Tomomi、Yamada Yusuke、Noguchi Ko
    • Journal Title

      Plant, Cell &amp; Environment

      Volume: 45 Issue: 1 Pages: 133-146

    • DOI

      10.1111/pce.14217

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arbuscular mycorrhizal fungal communities of a mangrove forest along a salinity gradient on Iriomote Island2021

    • Author(s)
      Akaji Yasuaki、Inoue Tomomi、Taniguchi Takeshi、Baba Shigeyuki
    • Journal Title

      Plant and Soil

      Volume: 472 Issue: 1-2 Pages: 145-159

    • DOI

      10.1007/s11104-021-05193-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distinct responses of growth and respiration to growth temperatures in two mangrove species2021

    • Author(s)
      Inoue Tomomi、Akaji Yasuaki、Noguchi Ko
    • Journal Title

      Annals of Botany

      Volume: 129 Issue: 1 Pages: 15-28

    • DOI

      10.1093/aob/mcab117

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The physiological potential of anammox bacteria as revealed by their core genome structure2020

    • Author(s)
      Okubo Takashi、Toyoda Atsushi、Fukuhara Kohei、Uchiyama Ikuo、Harigaya Yuhki、Kuroiwa Megumi、Suzuki Takuma、Murakami Yuka、Suwa Yuichi、Takami Hideto
    • Journal Title

      DNA Research

      Volume: 28 Issue: 1 Pages: 1-12

    • DOI

      10.1093/dnares/dsaa028

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 日本全国における森林渓流水水質の空間分布 / Spatial distribution of mountain streamwater chemistry in Japan2024

    • Author(s)
      牧野奏佳香,徳地直子,赤石大輔,小山里奈,駒井幸雄,板谷佳美,Nay Lin Maung
    • Organizer
      第135回日本森林学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Changes in Spatial Distribution of Stream Nitrate Concentrations throughout Japan over 20 Years: A Resurvey using Citizen Science2024

    • Author(s)
      Soyoka Makino, Naoko Tokuchi, Daisuke Akaishi, Lina Koyama, Yukio Komai, Yoshimi Itaya, Nay Lin Maung
    • Organizer
      Acid Rain 2020
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生育温度と土壌養分条件がヤエヤマヒルギの成長とガス交換特性に与える影響 Impacts of growing temperature and soil nutrient conditions on growth and gas exchange properties in Rhizophora stylosa2024

    • Author(s)
      河合清定, 赤路康朗, 井上智美, 小林正樹
    • Organizer
      日本生態学会第71回全国大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] マングローブ植物の呼吸と成長の塩分に対する応答は生育温度によって異なる Growth and respiratory responses of a mangrove plant Rhizophora stylosa to salinity depend on growth temperature2024

    • Author(s)
      井上智美, 藤村朋子, 野口航
    • Organizer
      日本生態学会第71回全国大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] マングローブ植物の地上根通気組織と窒素固定/Nitrogen diffusion and diazotrophic fixation through mangrove aerial roots2023

    • Author(s)
      井上智美, 高津文人, 赤路康朗, 下野綾子
    • Organizer
      日本生態学会第70回全国大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 微小透析法を用いた土壌の窒素可給性の評価:従来手法との比較の試み/ Evaluation of Soil Nitrogen Availability Using Microdialysis Method: An Attempt to Compare with Conventional Methods2023

    • Author(s)
      小山里奈
    • Organizer
      日本生態学会第70回全国大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] アナモックスと共在細菌の関係に着目したアナモックスアバンダンス制御 因子の探索2022

    • Author(s)
      黒岩 恵 , 村井 沙妃 , 川面 佑登 , 寺田 昭彦 , 利谷 翔平 , 安田 昌平 , 諏訪 裕一 , 高見 英人
    • Organizer
      日本地球惑星科学連合2022年大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ヒルギ科3種の葉と根の呼吸速度の温度依存性2022

    • Author(s)
      井上智美, 赤路康朗, 野口航
    • Organizer
      日本生態学会第69回全国大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 塩ストレスがヒルギ科マングローブ二種の菌根共生と養分吸収に及ぼす影響2022

    • Author(s)
      赤路康朗, 井上智美, 高津文人, 谷口武士
    • Organizer
      日本生態学会第69回全国大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Facilitation of soil nitrate production by mangrove roots in hypoxic soil condition2021

    • Author(s)
      Tamaki Morioka, Lina A. Koyama, Tomomi Inoue, Megumi Kuroiwa, Tomohiro Yokobe, Ryunosuke Tateno
    • Organizer
      AGU Fall Meeting 2021
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Book] Mangroves of Japan. In: Das, S.C., Pullaiah, Ashton, E.C. (eds) Mangroves: Biodiversity, Livelihoods and Conservation2022

    • Author(s)
      Inoue Tomomi、Kohzu Ayato、Akaji Yasuaki、Miura Shingo、Baba Shigeyuki、Oshiro Nozomi、Kezuka Mio、Kainuma Mami、Tokuoka Harumi、Naruse Tohru
    • Total Pages
      609
    • Publisher
      Springer, Singapore
    • ISBN
      9789811905186
    • Related Report
      2021 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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