• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Magentic sparation of antibiotics and application to anaerobic digesion for resistant bacteria pandemic in livestock environment

Research Project

Project/Area Number 20H03107
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 41040:Agricultural environmental engineering and agricultural information engineering-related
Research InstitutionKobe University

Principal Investigator

Ihara Ikko  神戸大学, 農学研究科, 教授 (50396256)

Co-Investigator(Kenkyū-buntansha) 間世田 英明  国立研究開発法人産業技術総合研究所, 生命工学領域, 上級主任研究員 (10372343)
清水 和哉  東洋大学, 生命科学部, 教授 (10581613)
梅津 一孝  帯広畜産大学, 畜産学部, 教授 (20203581)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2022: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2020: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords家畜糞尿 / 動物用抗菌剤 / 抗生物質 / 嫌気性消化 / 薬剤耐性菌 / 磁気分離 / 薬剤耐性 / 抗生物質耐性菌 / 畜産廃水 / 乳牛糞尿 / 耐性菌
Outline of Research at the Start

畜産業において,家畜の疾病治療・予防,そして生産性向上のために,動物用抗菌剤(抗生物質)が使用されている。近年,畜産由来の抗生物質耐性菌が環境に拡散し,伝播することから「サイレントパンデミック」として,その影響が懸念されている。本研究では,畜産施設からの排出される薬剤耐性菌に対し包括的な制御法を確立するため,家畜糞尿含有廃水や廃棄乳等の液相に残留する抗生物質の磁気分離による残留抗生物質除去と,嫌気性消化(メタン発酵)による家畜糞等の固相に残留する薬剤耐性菌の低減手法を開発する。

Outline of Final Research Achievements

In order to establish a method for controlling antibiotic-resistant bacteria discharged from livestock facilities, the removal of residual antibiotics by magnetic separation of antibiotics in wastewater containing animal manure and a method for reducing antibiotic-resistant bacteria by anaerobic digestion were investigated. Factors contributing to the removal rate were found in magnetic seeding of antibiotics in solutions containing high concentrations of organic matter, such as livestock manure solutions. Furthermore, the risk of emergence of antibiotic resistant bacteria, i.e. mutation risk of resistant genes, was assessed using actual wastewater, and dynamic changes in wastewater treatment performance and microbial communities were analyzed to find the minimum antibiotic concentration that would not affect the microbial community structure and wastewater treatment performance.

Academic Significance and Societal Importance of the Research Achievements

得られた研究成果は,ワンヘルスアプローチが期待される抗生物質耐性菌対策において畜産環境分野から直接的に貢献できる知見を含むことから,社会的な意義は大きいと考えられる。また,磁気力の環境応用を含むことから磁気科学の裾野を広げることにも寄与していると考えられ,学術的な意義も大きい。

Report

(4 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (33 results)

All 2024 2023 2022 2021 2020 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (24 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (1 results)

  • [Int'l Joint Research] 北京大学(中国)

    • Related Report
      2022 Annual Research Report
  • [Journal Article] 低温メタン発酵における乳牛糞尿に残留する抗生物質耐性病原菌の消長2024

    • Author(s)
      田中優花,岩崎匡洋,Farghali Mohamed, You Jingyi, 吉田弦,井原一高,Fetra J. Andriamanohiarisoamanana,清水和哉,間世田英明
    • Journal Title

      関西農業食料工学会会報

      Volume: 135 Pages: 34-35

    • Related Report
      2022 Annual Research Report
  • [Journal Article] 低温メタン発酵における乳牛糞尿に残留する抗生物質耐性菌の消長2023

    • Author(s)
      田中優花,Jingyi You,Fetra Andriamanohiarisoamanana,吉田弦,井原一高,清水和哉,間世田英明
    • Journal Title

      関西農業食料工学会会報

      Volume: 134 Pages: 6-6

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Emergence of multidrug-resistant Acinetobacter baumannii under fluctuating levofloxacin concentration and its control by chlorine and UV disinfection2023

    • Author(s)
      Zhang Chi、Hao Liting、Miao Hanchen、Chen Jiayu、Yuan Tian、Lei Zhongfang、Zhang Zhenya、Utsumi Motoo、Itayama Tomoaki、Miura Takeshi、Ihara Ikko、Maseda Hideaki、Tabassum Salma、Shimizu Kazuya
    • Journal Title

      Process Safety and Environmental Protection

      Volume: 173 Pages: 344-353

    • DOI

      10.1016/j.psep.2023.03.010

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Decreased Efficiency of Free Naked DNA Transformation by Chlorine and UV Disinfection and Its Detection Limitations2023

    • Author(s)
      Zhang Chi、Miao Hanchen、Lei Zhongfang、Yuan Tian、Zhang Zhenya、Ihara Ikko、Maseda Hideaki、Shimizu Kazuya
    • Journal Title

      Water

      Volume: 15 Issue: 6 Pages: 1232-1232

    • DOI

      10.3390/w15061232

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kinetic modeling of anaerobic co-digestion with glycerol: Implications for process stability and organic overloads2023

    • Author(s)
      Farghali Mohamed、Mohamed Israa M.A.、Hassan Dalia、Iwasaki Masahiro、Yoshida Gen、Umetsu Kazutaka、Ihara Ikko
    • Journal Title

      Biochemical Engineering Journal

      Volume: 199 Pages: 109061-109061

    • DOI

      10.1016/j.bej.2023.109061

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Comparative effects of ferric hydroxide and (semi) conductive iron oxides on the anaerobic digestion of oxytetracycline-contaminated dairy manure2022

    • Author(s)
      Andriamanohiarisoamanana, FJ., Ihara, I., Yoshida, G., and Umetsu, K.
    • Journal Title

      Journal of Environmental Management

      Volume: 310 Pages: 114731-114731

    • DOI

      10.1016/j.jenvman.2022.114731

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 電気化学磁気シーディングを用いた畜産排水からの抗生物質の磁気分離2021

    • Author(s)
      楢原優貴,井原一高,吉田弦,梅津一孝
    • Journal Title

      関西農業食料工学会支部報

      Volume: 130 Pages: 44-44

    • Related Report
      2021 Annual Research Report
  • [Presentation] 低温メタン発酵による乳牛ふん尿残留抗生物質耐性病原菌制御の検討2024

    • Author(s)
      井原一高,田中優花,岩崎匡洋,Farghali Mohamed, You Jingyi, 吉田弦, Fetra J. Andriamanohiarisoamanana,清水和哉,間世田英明
    • Organizer
      第58回日本水環境学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 家畜糞尿のメタン発酵におけるスルホンアミド系抗菌薬の低減に関する基礎検討2024

    • Author(s)
      山本英里,吉田弦,井原一高
    • Organizer
      関西農業食料工学会第151回例会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Effects of biochar as an additive on the survival of ARB in dairy manure during the batch mesophilic anaerobic digestion2023

    • Author(s)
      Jingyi You, Mohamed Farghali, Masahiro Iwasaki, Gen Yoshida, Fetra J. Andriamanohiarisoamanana, Kazuya Shimizu, Hideaki Maseda, Ikko Ihara
    • Organizer
      農業環境工学関連学会2023年合同大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 高濃度有機廃液からの抗生物質除去のための電気化学凝集における鉄溶出手法の検討2023

    • Author(s)
      鈴木崇矢,吉田弦,井原一高
    • Organizer
      第26回日本水環境学会シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] リスク物質の濃縮回収のための球状磁気フィルタを用いた磁気分離プロセス2023

    • Author(s)
      江村寛,吉田弦,井原一高
    • Organizer
      第106回低温工学・超電導学会研究発表会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 家畜糞尿液分に含有する抗生物質の磁気分離 -電気化学磁気シーディングにおける溶液pH の影響-2022

    • Author(s)
      楢原優貴,Fetra Jules Andriamanohiarisoamanana, 吉田弦,井原一高,梅津一孝
    • Organizer
      2022 年農業施設学会 学生・若手研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Effect of anaerobic digestion phase on the survival of antibioticresistant bacteria during the batch mesophilic anaerobic digestion with cefazolin-spiked dairy cow manure2022

    • Author(s)
      You Jingyi, Ihara Ikko, Yoshida Gen, Fetra J Andriamanohiarisoamanana, Shimizu Kazuya, Maseda Hideaki, Umetsu Kazutaka
    • Organizer
      2022 年農業施設学会 学生・若手研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] リスク物質処理のための磁気フィルタによる分離・回収プロセスの検討2022

    • Author(s)
      江村寛,吉田弦,井原一高
    • Organizer
      関西農業食料工学会 第147回例会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 不溶性アノードを用いた廃棄乳に含有する動物用抗菌剤の分解2022

    • Author(s)
      野村匠,陳海博,Fetra J. Andriamanohiarisoamanana, 吉田弦,井原一高,梅津一孝
    • Organizer
      関西農業食料工学会 第147回例会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 電気化学凝集による乳牛糞尿液分からのテトラサイクリン系抗生物質の分離除去2022

    • Author(s)
      鈴木崇矢,Fetra J. Andriamanohiarisoamanana, 吉田弦,井原一高,梅津一孝
    • Organizer
      関西農業食料工学会 第147回例会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Adaptation of microbial communities against antibiotics exposure during wastewater treatment processes2022

    • Author(s)
      Rui Xiao, Chi Zhang, Tian Yuan, Zhongfang Lei, Zhenya Zhang, Tomoaki Itayama, Ikko Ihara, Hideaki Maseda, Motoo Utsumi, Kazuya Shimizu
    • Organizer
      第56回日本水環境学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 電気化学凝集による家畜糞尿液分からの抗生物質の除去と磁気分離への応用2021

    • Author(s)
      楢原優貴,Fetra J. Andriamanohiarisoamanana, 吉田弦,井原一高,梅津一孝
    • Organizer
      第24 回日本水環境学会シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] Survival of Antibiotic Resistant Bacteria during Mesophilic Anaerobic Digestion with Cefazolin-Spiked Dairy Cow Manure2021

    • Author(s)
      You Jingyi, Gen Yoshida, Fetra J. Andriamanohiarisoamanana, Ikko Ihara, Kazuya Shimizu, Hideaki Maseda, Kazutaka Umetsu
    • Organizer
      2021年度農業施設学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Effect of Cefazolin on the Survival of Antibiotic Resistant Bacteria during the Batch Mesophilic Anaerobic Digestion of Dairy Cow Manure2021

    • Author(s)
      J.Y. You, I. Ihara, G. Yoshida, F. Andriamanohiarisoamanana, K. Shimizu, H. Maseda, K. Umetsu
    • Organizer
      The 13th Japan-China-Korea International Postgraduate Academic Symposium (Online Conference)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effectiveness of disinfection against multi-drug resistant bacteria and DA in wastewater treatment2021

    • Author(s)
      1)Chi Zhang, Hanchen Miao, Liting Hao, Zhenya Zhang, Zhongfang Lei, Tian Yuan, Hideaki Maseda, Ikko Ihara, Kazuya Shimizu
    • Organizer
      日本水処理生物学会第57回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 動物用抗菌剤の環境拡散防止のための磁気分離および嫌気性消化2021

    • Author(s)
      井原一高
    • Organizer
      第55回緑膿菌感染症研究会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 電気化学磁気シーディングを用いた畜産廃水からの抗生物質の磁気分離-溶液pHの影響-2021

    • Author(s)
      楢原優貴,井原一高,吉田弦,梅津一孝
    • Organizer
      関西農業食料工学会第145回例会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 畜産廃水の現状とアンモニア回収技術への期待2021

    • Author(s)
      井原一高
    • Organizer
      膜による廃水アンモニア分離ワークショップ
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 磁気力制御による畜産廃水からの抗生物質の選択分離2021

    • Author(s)
      井原一高,吉田朋高,Fetra ANDRIAMANOHIARISOAMANANA,吉田弦
    • Organizer
      第55回日本水環境学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Effect of cefazolin on the survival of antibiotic resistant bacteria during batch mesophilic anaerobic digestion with dairy cow manure2021

    • Author(s)
      You Jingyi, Gen Yoshida, Fetra Andriamanohiarisoamanana, Ikko Ihara, Kazuya Shimizu, Hideaki Maseda, Kazutaka Umetsu
    • Organizer
      第55回日本水環境学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Effect of Cefazolin Residue in Dairy Manure on Survival of Antibiotic-Resistant Bacteria during Mesophilic Anaerobic Digestion2021

    • Author(s)
      Ikko Ihara, Mari Seko, Fetra J. Andriamanohiarisoamanana, Gen Yoshida, Kazuya Shimizu, Hideaki Maseda, Masahiro Iwasaki, Kazutaka Umetsu
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment (Livestock Waste 2020)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Tackling Antibiotic Inhibition in Anaerobic Digestion: The Roles of Fe3+ and Fe3O4 on Process Performances and Volatile Fatty Acids Utilization Pattern2021

    • Author(s)
      Fetra Andriamanohiarisoamanana, Ikko Ihara, Gen Yoshida, Kazutaka Umetsu
    • Organizer
      The 4th International Conference on Recent Advancements in Sustainable Management of Livestock Waste and Rural Environment (Livestock Waste 2020)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 電気化学凝集を活用した酪農廃水からの抗生物質の磁気分離2020

    • Author(s)
      井原一高,岩崎光一郎,吉田弦,梅津一孝
    • Organizer
      第23回日本水環境学会シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 磁気力制御による畜産廃水に含有する抗生物質の濃縮分離と回収に関する検討2020

    • Author(s)
      吉田朋高,吉田弦,Fetra Andriamanohiarisoamanana,井原一高,梅津一孝
    • Organizer
      2020年農業施設学会秋季学生・若手研究発表会
    • Related Report
      2020 Annual Research Report
  • [Book] 農業・食料生産分野における バイオマス利用工学 - 循環型社会のための生物資源利用2023

    • Author(s)
      井原一高(分担執筆)
    • Total Pages
      326
    • Publisher
      コロナ社
    • Related Report
      2022 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi