| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2023: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2022: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2020: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
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| Outline of Final Research Achievements |
(1) In Euglena wax ester synthesis, transhydrogenase (NNT) bound to the inner mitochondrial membrane is essential for wax ester synthesis and anaerobic ATP synthesis, and contributes to recycling of reduced cofactors. Furthermore, NNT and the malate transport pathway function to convert NADPH generated in mitochondria to NADH, which can be supplied for anaerobic respiration. (2) Stable knockout mutants of the enzymes of the fatty acid β-oxidation reversal pathway were produced, and a number of stable mutants with different wax ester compositions from the wild strain were generated. (3) We succeeded in developing a stable nuclear genome transformation method for Euglena. We will continue to improve the system to a level where it can be used for metabolic engineering.
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