| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2023: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Outline of Final Research Achievements |
The mismatch repair system handles both DNA biosynthesis and recombination errors to increase the fidelity in DNA replication and recombination. While these reactions are triggered by the same mismatch sensors, the downstream events are highly diverged. Importantly, the molecular mechanism of recombination regulation by the mismatch repair system remains ambiguous, and the pathway-choice mechanism of the two pathways is also poorly understood. In this study, we established biochemical model systems that recapitulate the regulation of recombination using Xenopus egg nucleoplasmic extracts and purified proteins. Using these systems, we identified factors that affect the fidelity of recombination. These results contribute to the basic understanding of genome maintenance.
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