| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2022: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2021: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2020: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
We found a critical CENP-A residue essential for faithful chromosome segregation. Moreover, we demonstrated that this residue was required for the proper complex formation with the CENP-A specific chaperon HJURP. Based on the structural analyses, we identified several contact sites of KNL2 to make a complex with CENP-A nucleosome, which was essential for the KNL2 localization and function. Furthermore, the artificial ectopic tethering assays indicated the additional CENP-A loading pathway depending on the CENP-C interaction with HJURP. These findings may provide insight into developing an anti-cancer drug utilizing centromeric chromatin modulation.
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