• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanism of growth inhibition by overexpression explored from the expression level of the limiting mutant protein

Research Project

Project/Area Number 20H03242
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43060:System genome science-related
Research InstitutionOkayama University

Principal Investigator

Moriya Hisao  岡山大学, 環境生命自然科学学域, 教授 (60500808)

Co-Investigator(Kenkyū-buntansha) 牧野 能士  東北大学, 生命科学研究科, 教授 (20443442)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2023: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords酵母 / 過剰発現 / タンパク質 / 細胞毒性 / タンパク質毒性 / プロテオスタシス / システムバイオロジー / 量感受性遺伝子 / 増殖阻害
Outline of Research at the Start

タンパク質の過剰発現は細胞機能に様々な悪影響を及ぼすが、そのメカニズムはほとんど分かっていない。本研究では、出芽酵母をモデル生物として、わずかに過剰発現しただけで増殖阻害を起こす「量感受性タンパク質」を対象とし、それらがどのようなメカニズムで増殖阻害を起こすのかを調査する。特に本研究では、これらのタンパク質の機能欠失変異体を体系的調査することで、過剰発現による増殖阻害のメカニズムを体系化する。

Outline of Final Research Achievements

Overexpression of proteins can harm cells in various ways. Using budding yeast as a model for eukaryotic cells, we have investigated the characteristics of protein groups that cause growth inhibition when overexpressed and have classified the mechanisms of growth inhibition. In this study, we further explored the toxicity mechanisms by introducing mutations into proteins that cause growth inhibition. As a result, we revealed that the presence of specific amino acids or the activity of the protein itself can lead to growth inhibition.

Academic Significance and Societal Importance of the Research Achievements

本研究は、これまでほとんど分かっていないタンパク質の発現を制約するメカニズムの解明につながり、基礎生物学的な知識の拡張に繋がる。また、ここで得られた知識は、細胞にタンパク質が蓄積することにより生じる疾患、癌や神経変性疾患などの細胞の生理状態の理解と創薬へと繋がるとともに、細胞工学において特定のタンパク質を大量発現させたいときのタンパク質デザインの指針となる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (15 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (4 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Remarks (6 results)

  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Protein sorting upon exit from the endoplasmic reticulum dominates Golgi biogenesis in budding yeast2024

    • Author(s)
      Sasaki Saku、Schlarmann Philipp、Hanaoka Kazuki、Nishii Hinako、Moriya Hisao、Mu?iz Manuel、Funato Kouichi
    • Journal Title

      FEBS Letters

      Volume: 598 Issue: 5 Pages: 548-555

    • DOI

      10.1002/1873-3468.14830

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Overexpression profiling reveals cellular requirements in the context of genetic backgrounds and environments2023

    • Author(s)
      Saeki Nozomu、Yamamoto Chie、Eguchi Yuichi、Sekito Takayuki、Shigenobu Shuji、Yoshimura Mami、Yashiroda Yoko、Boone Charles、Moriya Hisao
    • Journal Title

      PLOS Genetics

      Volume: 19 Issue: 4 Pages: e1010732-e1010732

    • DOI

      10.1371/journal.pgen.1010732

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Demonstration of iodide-dependent UVA-triggered growth inhibition in Saccharomyces cerevisiae cells and identification of its suppressive molecules2023

    • Author(s)
      Ono Ryota、Saeki Nozomu、Kojima Keiichi、Moriya Hisao、Sudo Yuki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 677 Pages: 1-5

    • DOI

      10.1016/j.bbrc.2023.07.048

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Massive expression of cysteine-containing proteins causes abnormal elongation of yeast cells by perturbing the proteasome2022

    • Author(s)
      Namba Shotaro、Kato Hisaaki、Shigenobu Shuji、Makino Takashi、Moriya Hisao
    • Journal Title

      G3 Genes|Genomes|Genetics

      Volume: - Issue: 6

    • DOI

      10.1093/g3journal/jkac106

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of uncharacterized proteins potentially localized to mitochondria (UPMs) in <scp> <i>Saccharomyces cerevisiae</i> </scp> using a fluorescent protein unstable in the cytoplasm2021

    • Author(s)
      Horiuchi Satoshi、Namba Shotaro、Saeki Nozomu、Satoh Ayano、Moriya Hisao
    • Journal Title

      Yeast

      Volume: 39 Issue: 5 Pages: 303-311

    • DOI

      10.1002/yea.3685

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Remarks] 岡山大学細胞システム化学研究室

    • URL

      https://tenure5.vbl.okayama-u.ac.jp/HMlab/

    • Related Report
      2022 Annual Research Report
  • [Remarks] 酵母とシステムバイオロジーブログ

    • URL

      https://tenure5.vbl.okayama-u.ac.jp/HM_blog/

    • Related Report
      2022 Annual Research Report
  • [Remarks] 岡山大学 細胞システム化学 守屋研究室

    • URL

      https://tenure5.vbl.okayama-u.ac.jp/HMlab/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 酵母とシステムバイオロジー

    • URL

      https://tenure5.vbl.okayama-u.ac.jp/HM_blog/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 岡山大学システム細胞学研究室

    • URL

      https://tenure5.vbl.okayama-u.ac.jp/HMlab/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 酵母とシステムバイオロジー(ブログ)

    • URL

      https://tenure5.vbl.okayama-u.ac.jp/HM_blog/

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi