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Elucidation of primary cell wall dynamics to regulate water conductivity of xylem vessel cells

Research Project

Project/Area Number 20H03271
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionThe University of Tokyo

Principal Investigator

Ohtani Misato  東京大学, 大学院新領域創成科学研究科, 教授 (60435633)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2021: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2020: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords植物細胞壁 / 非セルロース性多糖 / ペクチン / 道管 / 道管細胞 / 細胞壁
Outline of Research at the Start

陸上維管束被子植物にとって、水や無機塩類を輸送する道管細胞の機能制御は非常に重要である。これまで道管細胞の通水能力制御についてはリグニン化した二次細胞壁に注目が集まり、一次細胞壁の役割については情報が乏しいのが現状である。そこで本研究では、植物細胞の一次細胞壁に特徴的な非セルロース細胞壁多糖ペクチンに焦点をあて、道管細胞機能制御におけるペクチンダイナミクス制御の役割を明らかにする。さらに、基部陸上植物や仮道管をもつ裸子植物との比較発生進化学的解析を行い、ペクチン制御による細胞機能制御が植物進化上どのように獲得されてきたのか、制御分子プログラムの進化的変遷を明らかにする。

Outline of Final Research Achievements

Functional regulation of xylem vessels, which transport water and inorganic salts, is important for land vascular plants. In this study, I examined the role of plant primary cell wall-specific non-cellulosic cell wall polysaccharides in the regulation of xylem vessel function. As a result, novel genes for cell wall polysaccharide modification enzyme were successfully identified as regulators of xylem vessel functions. Moreover, the possibility that these enzymes would be functionally regulated at the cell wall rather than within the cell was demonstrated. These are significant results that provide new insights into the molecular mechanisms and importance of the regulation of primary wall dynamics in functional regulation of xylem vessels.

Academic Significance and Societal Importance of the Research Achievements

植物細胞壁に含まれる非セルロース性多糖は工業的に重要なバイオリソースであり、例えば一次壁に多く含まれるペクチンは食品工業で重用される重要多糖である。本研究で見出された新規の非セルロース性多糖修飾・分解酵素は、食品工業的新技術や次世代型バイオマテリアル開発への応用展開可能な基盤となりうる。さらに道管機能性は植物の成長を直接左右する重要な形質であり、本研究成果は作物や穀物成長制御技術開発に繋がる高い応用性を有している。

Report

(3 results)
  • 2023 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (25 results)

All 2022 2021 2020 Other

All Int'l Joint Research (3 results) Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 6 results) Presentation (13 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results)

  • [Int'l Joint Research] Universite de Picardie Jules Verne(フランス)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of British Columbia(カナダ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Universite de Picardie Jules Verne(フランス)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Plant secondary cell wall proteome analysis with an inducible system for xylem vessel cell differentiation2022

    • Author(s)
      Arae T, Nakakoji M, Noguchi M, Kamon E, Sano R, Demura T, Ohtani M
    • Journal Title

      Development, Growth & Differentiation

      Volume: 64 Issue: 1 Pages: 5-15

    • DOI

      10.1111/dgd.12767

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Histone deacetylation controls xylem vessel cell differentiation via transcriptional regulation of a transcription repressor complex OFP1/4-MYB75-KNAT7-BLH6.2022

    • Author(s)
      Hirai R, Wang S, Demura T, Ohtani M
    • Journal Title

      Frontiers in Plant Sciences

      Volume: 12 Pages: 825810-825810

    • DOI

      10.3389/fpls.2021.825810

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Enhancement of secondary cell wall formation in poplar xylem using a self-reinforced system of secondary cell wall-related transcription factors.2022

    • Author(s)
      Nakano Y, Endo H, Gerber L, Hori C, Ihara A, Sekimoto M, Matsumoto T, Kikuchi J, Ohtani M, Demura T
    • Journal Title

      Frontiers in Plant Sciences

      Volume: 13 Pages: 819360-819360

    • DOI

      10.3389/fpls.2022.819360

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Calcium signaling contributes to xylem vessel cell differentiation via post-transcriptional regulation of VND7 downstream events2021

    • Author(s)
      Kamon E, Noda C, Higaki T, Demura T, Ohtani M
    • Journal Title

      Plant Biotechnology

      Volume: 38 Issue: 3 Pages: 331-337

    • DOI

      10.5511/plantbiotechnology.21.0519a

    • NAID

      130008091606

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2021-09-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional analysis of poplar SOMBRERO-type NAC transcription factors yields strategy to modify woody cell wall properties.2021

    • Author(s)
      Akiyoshi N, Ihara A, Matsumoto T, Takebayashi A, Hiroyama R, Kikuchi J, Demura T, Ohtani M
    • Journal Title

      Plant & Cell Physiology

      Volume: 62 Issue: 12 Pages: 1963-1974

    • DOI

      10.1093/pcp/pcab102

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Xylem vessel cell differentiation: a best model for new integrative cell biology?2021

    • Author(s)
      Kamon E, Ohtani M
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 64 Pages: 102135-102135

    • DOI

      10.1016/j.pbi.2021.102135

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Mechanics and Biology of Plant Cell Walls: Resilience and Sustainability for Our Future Society2021

    • Author(s)
      Ohtani Misato、Kotake Toshihisa、Mortimer Jenny C、Demura Taku
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 12 Pages: 1787-1790

    • DOI

      10.1093/pcp/pcab168

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] オミクス解析から解き明かす木質形成機構2021

    • Author(s)
      大谷美沙都
    • Journal Title

      アグリバイオ

      Volume: 5 Pages: 13-17

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Plant-specific Dof transcription factors VASCULAR-RELATED DOF1 and VASCULAR-RELATED DOF2 regulate vascular cell differentiation and lignin biosynthesis in Arabidopsis2020

    • Author(s)
      Ramachandran Vasagi、Tobimatsu Yuki、Masaomi Yamamura、Sano Ryosuke、Umezawa Toshiaki、Demura Taku、Ohtani Misato
    • Journal Title

      Plant Molecular Biology

      Volume: 104 Issue: 3 Pages: 263-281

    • DOI

      10.1007/s11103-020-01040-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] Proteomic analysis of plant secondary cell wall with an inducible system for xylem vessel cell differentiation2022

    • Author(s)
      Arae T, Nakakoji M, Noguchi M, Kamon E, Demura T, Ohtani M
    • Organizer
      Kyoto University / ERATO International Symposium Chemistry and Plant Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Engineering of lignocellulosic biomass by key transcription factors for woody cell formation2022

    • Author(s)
      Akiyoshi N, Nakano Y, Gerber L, Hori C, Kikuchi J, Demura T, Ohtani M
    • Organizer
      Kyoto University / ERATO International Symposium Chemistry and Plant Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物の細胞分化を制御する転写後遺伝子発現調節の解明2022

    • Author(s)
      大谷美沙都
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 通水細胞形成マスター転写因子 VNS ファミリーのシス配列結合親和性の分子的進化2022

    • Author(s)
      秋吉信宏、田村泰造、出村拓、大谷美沙都
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 木部道管細胞の二次細胞壁形成における細胞膜健全性の重要性2022

    • Author(s)
      家門絵理、野田千尋、出村拓、大谷美沙都
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Crucial role for protein post-translational modification to regulate secondary wall formation induced by VND7.2021

    • Author(s)
      3.Ohtani M, Kawabe H, Phookaew P, Suzuki T, Ogawa Y, Akiyoshi N, Sano R, Takebayashi A, Nakagami H, Demura T
    • Organizer
      The 7th International Conference on Plant Cell Wall Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Proteomic analysis of cell wall proteins during xylem cell differentiation based on multiple protein extraction methods.2021

    • Author(s)
      Arae T, Nakakoji M, Noguchi M, Demura T, Ohtani M
    • Organizer
      The 7th International Conference on Plant Cell Wall Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of poplar SOMBRERO-type NAC transcription factors yields strategy to modify woody cell wall properties.2021

    • Author(s)
      Akiyoshi N, Ihara A, Matsumoto T, Takebayashi A, Hiroyama R, Kikuchi J, Demura T, Ohtani M
    • Organizer
      The 7th International Conference on Plant Cell Wall Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chemical biological analysis of cortical microtubule dynamics regulating secondary cell wall pattern formation.2021

    • Author(s)
      Kamon E, Yoneda A, Tsugawa S, Ohtani M, Demura T.
    • Organizer
      The 7th International Conference on Plant Cell Wall Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 道管細胞特異的ポリガラクツロナーゼ変異体が示す生育阻害と物質輸送の関連性2021

    • Author(s)
      生田風馬、秋吉信宏、野田口理孝、出村拓、大谷美沙都
    • Organizer
      第85回日本植物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] オウシュウトウヒ培養細胞を用いた仮道管形成関連遺伝子の解析2020

    • Author(s)
      秋吉 信宏、佐野 亮輔、山岸 祐介、船田 良、出村 拓、大谷 美沙都
    • Organizer
      第84回日本植物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 二次細胞壁パターン形成を制御する表層微小管動態に関する化学生物学的解析2020

    • Author(s)
      家門 絵理、津川 暁、米田 新、Sean Cutler、橋本 隆、大谷 美 沙都、出村 拓
    • Organizer
      第84回日本植物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナ道管細胞分化マスター制御転写因子 VND7の機能調節におけるタンパク質翻訳後修飾の重要性2020

    • Author(s)
      大谷 美沙都、Phookaew Pawittra、川邊 陽文、鈴木 崇臣、佐野 亮輔、竹林 有理佳、中神 弘史、出村 拓
    • Organizer
      第84回日本植物学会年会
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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