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Sugar metabolism regulation by a photosynthesis-signaling factor and its evolution

Research Project

Project/Area Number 20H03275
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionTokyo University of Science

Principal Investigator

Nishihama Ryuichi  東京理科大学, 理工学部応用生物科学科, 教授 (70283455)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2022: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2021: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2020: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
KeywordsRaf様キナーゼ / 光合成 / 糖代謝 / ゼニゴケ / シグナル伝達 / 成長制御 / 液-液相分離 / 光合成シグナリング / スクロース / デンプン / 炭素分配
Outline of Research at the Start

光合成と成長を協調させる調節ネットワークの解明は植物科学の主要な課題と言えるが、複雑であり、未解明な部分が多く残されている。申請者はこれまでに、光合成刺激を受けてリン酸化され、ショ糖代謝を制御することで炭素分配や成長を調節する新規光合成シグナル伝達因子PRAFを同定し、解析を進めてきた。しかしながら、その活性化機構や基質、局在の詳細、進化における機能共通性などはよくわかっていない。そこで本研究では、光合成シグナルによるPRAFの機能制御機構の解明、PRAFの進化的保存性の解明、新規光合成シグナル伝達因子の同定を目的とした解析を行う。ショ糖代謝制御機構に関する重要な知見が得られると期待される。

Outline of Final Research Achievements

The B4-type Raf-like kinase PRAF functions in coordinating growth and metabolism with photosynthetic activity in Marchantia polymorpha. PRAF is phosphorylated in a photosynthetic stimulus-dependent manner. Disruption of the PRAF gene causes sugar metabolism defects and severely inhibits growth. In this study, molecular mechanisms of this signaling were investigated. We demonstrated that multimerization with other PRAF molecules via the multimerization module PB1 domain present at the N-terminus of the protein is essential for its own phosphorylation and function. PRAF forms granular structures. We obtained evidence suggesting that granules are formed by phase separation. In addition, candidate target proteins of PRAF were identified.

Academic Significance and Societal Importance of the Research Achievements

光合成を行う植物にとって、成長や代謝などの生命現象を光合成の活性と調和させる必要がある。しかしその仕組みは多様であり、解明すべき点が数多く残されている。本研究では、光合成駆動の刺激を伝えるタンパク質リン酸化酵素PRAFの解析を通して、その一端を明らかにした。植物は進化を通してPRAFを保持しているため、その機能は植物に共通したものであると考えられ、本研究の成果は農業、園芸、環境保全等に活用できる知見であると考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (34 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (3 results) Journal Article (12 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 12 results,  Open Access: 9 results) Presentation (16 results) Remarks (3 results)

  • [Int'l Joint Research] Wageningen University(オランダ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Wageningen University(オランダ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Wageningen University(オランダ)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Stomatal regulators are co-opted for seta development in the astomatous liverwort Marchantia polymorpha2023

    • Author(s)
      Moriya Kenta C.、Shirakawa Makoto、Loue-Manifel Jeanne、Matsuda Yoriko、Lu Yen-Ting、Tamura Kentaro、Oka Yoshito、Matsushita Tomonao、Hara-Nishimura Ikuko、Ingram Gwyneth、Nishihama Ryuichi、Goodrich Justin、Kohchi Takayuki、Shimada Tomoo
    • Journal Title

      Nature Plants

      Volume: 9 Issue: 2 Pages: 302-314

    • DOI

      10.1038/s41477-022-01325-5

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] CRISPR/Cas9-mediated disruption of <i>ALLENE OXIDE SYNTHASE</i> results in defective 12-oxo-phytodienoic acid accumulation and reduced defense against spider mite (<i>Tetranychus urticae</i>) in liverwort (<i>Marchantia polymorpha</i>)2022

    • Author(s)
      Koeduka, T., Takaishi, M., Suzuki, M., Nishihama, R., Kohchi, T., Uefune, M., and Matsui, K.
    • Journal Title

      Plant Biotechnology

      Volume: 39 Issue: 2 Pages: 191-194

    • DOI

      10.5511/plantbiotechnology.22.0328a

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2022-06-25
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Auxin signaling is essential for organogenesis but not for cell survival in the liverwort<i>Marchantia polymorpha</i>2022

    • Author(s)
      Suzuki Hidemasa、Kato Hirotaka、Iwano Megumi、Nishihama Ryuichi、Kohchi Takayuki
    • Journal Title

      The Plant Cell

      Volume: 35 Issue: 3 Pages: 1058-1075

    • DOI

      10.1093/plcell/koac367

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] MarpolBase Expression: A Web-Based, Comprehensive Platform for Visualization and Analysis of Transcriptomes in the Liverwort <i>Marchantia polymorpha</i>2022

    • Author(s)
      Kawamura Shogo、Romani Facundo、Yagura Masaru、Mochizuki Takako、Sakamoto Mika、Yamaoka Shohei、Nishihama Ryuichi、Nakamura Yasukazu、Yamato Katsuyuki T、Bowman John L、Kohchi Takayuki、Tanizawa Yasuhiro
    • Journal Title

      Plant and Cell Physiology

      Volume: 63 Issue: 11 Pages: 1745-1755

    • DOI

      10.1093/pcp/pcac129

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Normal oil body formation in Marchantia polymorpha requires functional coat protein complex I proteins2022

    • Author(s)
      Kanazawa T, Nishihama R, Ueda T
    • Journal Title

      Frontiers in Plant Science

      Volume: 13 Pages: 979066-979066

    • DOI

      10.3389/fpls.2022.979066

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Renaissance and Enlightenment of Marchantia as a model system2022

    • Author(s)
      Bowman, J.L., Berger,F., Briginshaw, L.N., Davies, K.M., Dierschke, T., Dolan,L., Fisher, T.J., Flores-Sandoval,E., Futagami,K., Ishizaki, K., Kato, H., Kohchi, T., Levins, J., Lin, S-S., Nishihama, R., Romani, R., Tanizawa, Y., Tsuzuki, M., Watanabe,Y., Yamato, K.T., Zachgo, S.
    • Journal Title

      The Plant Cell

      Volume: 34 Issue: 10 Pages: 3512-3542

    • DOI

      10.1093/plcell/koac219

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Protein Kinase MpYAK1 Is Involved in Meristematic Cell Proliferation, Reproductive Phase Change and Nutrient Signaling in the Liverwort Marchantia polymorpha2022

    • Author(s)
      Haruka Shinkawa; Masataka Kajikawa; Tomoyuki Furuya; Ryuichi Nishihama; Hirokazu Tsukaya; Takayuki Kohchi; Hideya Fukuzawa
    • Journal Title

      Plant and Cell Physiology

      Volume: 63 Issue: 8 Pages: 1063-1077

    • DOI

      10.1093/pcp/pcac076

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A plant-specific DYRK kinase DYRKP coordinates cell morphology in Marchantia polymorpha.2021

    • Author(s)
      Furuya T; Shinkawa H; Kajikawa M; Nishihama R; Kohchi T; Fukuzawa H; Tsukaya H
    • Journal Title

      Journal of plant research

      Volume: 134 Issue: 6 Pages: 1265-1277

    • DOI

      10.1007/s10265-021-01345-w

    • NAID

      40022725207

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] <i>Agrobacterium</i>-Mediated Transient Transformation of Marchantia Liverworts2021

    • Author(s)
      Iwakawa Hidekazu、Melkonian Katharina、Schl?ter Titus、Jeon Hyung-Woo、Nishihama Ryuichi、Motose Hiroyasu、Nakagami Hirofumi
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 11 Pages: 1718-1727

    • DOI

      10.1093/pcp/pcab126

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Diversified amino acid-mediated allosteric regulation of phosphoglycerate dehydrogenase for serine biosynthesis in land plants2021

    • Author(s)
      E. Okamura, K. Ohtaka, R. Nishihama, K. Uchida, A. Kuwahara, K. Mochida. M. Yokota Hirai
    • Journal Title

      Biochemical Journal

      Volume: 478 Issue: 12 Pages: 2217-2232

    • DOI

      10.1042/bcj20210191

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fungal-Type Terpene Synthases in Marchantia polymorpha Are Involved in Sesquiterpene Biosynthesis in Oil Body Cells2021

    • Author(s)
      Takizawa Ryosuke、Hatada Miki、Moriwaki Yuta、Abe Sachika、Yamashita Yuko、Arimitsu Ryoma、Yamato Katsuyuki T、Nishihama Ryuichi、Kohchi Takayuki、Koeduka Takao、Chen Feng、Matsui Kenji
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 3 Pages: 528-537

    • DOI

      10.1093/pcp/pcaa175

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Kinase in the liverwort Marchantia polymorpha; A photosynthesis-dependent kinase for carbon metabolism2020

    • Author(s)
      Koide E, Suetsugu N, Iwano M, Gotoh E, Nomura Y, Stolze SC, Nakagami H, Kohchi T, Nishihama R
    • Journal Title

      Plant Cell & Physiology

      Volume: 61 Issue: 3 Pages: 631-643

    • DOI

      10.1093/pcp/pcz232

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ゼニゴケにおける細胞周期制御因子 WEE1 と CDC25 の機能解析2023

    • Author(s)
      小田歩美,安喜史織,西浜竜一,梅田正明
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ゼニゴケの細胞核光定位運動におけるキネシン様タンパク質 MpKAC の機能的役割2023

    • Author(s)
      岩渕功誠,八木宏樹,沖奈那夏,横畑伶奈,中田亜紗美,広本沙耶,小松愛乃,酒井友希,高木慎吾,西浜竜一,河内孝之,渡辺洋平,上田晴子,西村いくこ
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 青色光に応答した細胞膜 H+-ATPase の 2 つの部位のリン酸化は気孔開口に必須である2023

    • Author(s)
      冨士彩紗,山内翔太,杉山直幸,西浜竜一,島崎研一郎,武宮淳史
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ゼニゴケにおける光合成シグナル伝達因子 MpPRAF の光刺激依存的な顆粒形成2023

    • Author(s)
      半田和華,新宅明日架,小出絵理,岩野惠,河内孝之,西浜竜一
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Marpolbase: ゼニゴケのための遺伝子情報および発現量データベースの開発2023

    • Author(s)
      谷澤靖洋,川村省吾,Facundo Romani,矢倉勝,望月孝子,坂本美佳,山岡尚平,西浜竜一,中村保一,大和勝幸,John Bowman,河内孝之
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 気孔をもたないコケ植物ゼニゴケにおける気孔形成関連遺伝子の機能解析2022

    • Author(s)
      守屋健太,白川一,Jeanne Loue-Manifel,松田頼子,田村謙太郎,岡義人,松下智直,西村いくこ,Gwyneth Ingram,西浜竜一,Justin Goodrich,河内孝之,嶋田知生
    • Organizer
      日本進化学会第24回年大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] MarpolBase Expression (MBEX): 苔類ゼニゴケにおけるトランスクリプトームの可視化・解析データベース2022

    • Author(s)
      川村昇吾,Facundo Romani,矢倉勝,望月孝子,坂本美佳,山岡尚平,西浜竜一,中村保一,大和勝幸,John Bowman,河内孝之,谷澤靖洋
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 陸上植物におけるガス交換系の発生と環境への適応2022

    • Author(s)
      水谷未耶,内橋貴之,Christian Ganser,林優紀,大川諒也,大西(小川)真理,松林嘉克,西浜竜一,木下俊則,石崎公庸,河内孝之,東山哲也,金岡雅浩
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ゼニゴケの class II AS2ファミリー遺伝子の解析2022

    • Author(s)
      小島晶子,西浜竜一,石田咲子,安藤沙友里,河内孝之,町田千代子,町田泰則
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ゼニゴケB4型Raf様キナーゼMpPRAFの光合成刺激依存的リン酸化と局在の制御機構2022

    • Author(s)
      西浜竜一,新宅明日架,半田和華,小出絵里,岩野惠,河内孝之
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ゼニゴケのタンパク質リン酸化酵素MpYAK1は栄養生殖,生殖器誘導および窒素欠乏応答の制御に関与する2022

    • Author(s)
      新川はるか,梶川昌孝,古谷朋之,西浜竜一,塚谷裕一,河内孝之,福澤秀哉
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 苔類ゼニゴケの生殖器托形成過程におけるMID1-COMPLEMENTING ACTIVITY オルソログの機能解析2022

    • Author(s)
      岩野惠,末次憲之,西浜竜一,石田咲子,木村緑,飯田和子,飯田秀利,永井健治,河内孝之
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 陸上植物における細胞核光定位運動の多様性と共通性2022

    • Author(s)
      岩渕功誠,八木宏樹,守屋健太,沖奈那夏,横畑伶奈,中田亜紗美,広本沙耶,小松愛乃,酒井友希,嶋田知生,高木慎吾,西浜竜一,河内孝之,渡辺洋平,上田晴子,西村いくこ
    • Organizer
      第63回 日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 光合成信号伝達キナーゼ PRAF の機能と局在2020

    • Author(s)
      小出絵理、新宅明日架、末次憲之、岩野惠、後藤栄治、野村有子、Sara C. Stolze、中神弘史、河内孝之、西浜竜一
    • Organizer
      近畿植物学会 第 9 回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] セリン生合成酵素3-phosphoglycerate dehydrogenase のアミノ酸によるアロステリック制御についての考察2020

    • Author(s)
      岡村英治、大高きぬ香、内田開、西浜竜一、桑原亜由子、持田恵一、平井優美
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 光合成依存の葉緑体光定位運動2020

    • Author(s)
      木原后紀、末次憲之、西浜竜一、河内孝之、後藤栄治
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Remarks] 気孔のない植物タイ類における気孔形成因子の役割を解明―コケ植物特有の組織「蒴柄」と気孔の意外な関係―

    • URL

      https://www.tus.ac.jp/today/archive/20230119_1189.html

    • Related Report
      2022 Annual Research Report
  • [Remarks] 東京理科大学理工学部応用生物科学科 西浜研究室ウェブサイト

    • URL

      https://www.rs.tus.ac.jp/nishihama/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 京都大学大学院生命科学研究科 遺伝子特性学分野ホームページ

    • URL

      https://www.plantmb.lif.kyoto-u.ac.jp/publications/

    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2025-03-27  

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