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Molecular basis of CO2 response in stomatal apparatus

Research Project

Project/Area Number 20H03279
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionKyushu University

Principal Investigator

Iba Koh  九州大学, 理学研究院, 名誉教授 (10192501)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2021: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2020: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords気孔 / CO2 / 二酸化炭素 / 野生系統
Outline of Research at the Start

本研究においては、CO2感知機構解明の鍵をにぎる膜交通を介した植物独自の動的細胞間コミュニケーション、光合成器官から情報処理装置へと進化した気孔葉緑体の特異な振舞い、さらにCO2感知のマスターレギュレータHT1に着目し、順遺伝学的アプローチをベースにライブセルイメージング・画像解析技術を駆使し、CO2センサの実体解明とCO2環境情報の処理機構について探求し、植物におけるCO2感知の分子基盤を解明する。

Outline of Final Research Achievements

Focusing on stomatal chloroplast-independent and drought-independent CO2 signalling pathways responsible for higher-order information processing in the stomatal apparatus, we sought to identify novel CO2 signalling and higher-order information processing factors. Proteomic analysis of isolated stomatal chloroplasts identified stomatal chloroplast factors involved in the regulation of stomatal opening and closing. Analysis of the causal gene of a CO2-insensitive mutant revealed that PE, a phospholipid abundant in pore cells, is essential for stomatal opening. Furthermore, thermal imaging mutant screening isolated a mutant with an abnormal ABA-independent stomatal closure response.

Academic Significance and Societal Importance of the Research Achievements

本研究は、植物における生体情報、環境情報の集積中枢となっている気孔装置における高次情報処理の仕組みを精査することによって、植物の生命力の強さ、すなわち頑健性の源となっている植物に特異的な細胞動態の解明をめざしている。

Report

(4 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (40 results)

All 2024 2023 2022 2021 2020 Other

All Int'l Joint Research (2 results) Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 4 results) Presentation (28 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results)

  • [Int'l Joint Research] UCSD(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] UCSD(米国)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] PECT1, a rate‐limiting enzyme in phosphatidylethanolamine biosynthesis, is involved in the regulation of stomatal movement in Arabidopsis2023

    • Author(s)
      Negi Juntaro、Obata Tomoki、Nishimura Sakura、Song Boseok、Yamagaki Sho、Ono Yuhei、Okabe Makoto、Hoshino Natsumi、Fukatsu Kohei、Tabata Ryo、Yamaguchi Katsushi、Shigenobu Shuji、Yamada Masashi、Hasebe Mitsuyasu、Sawa Shinichiro、Kinoshita Toshinori、Nishida Ikuo、Iba Koh
    • Journal Title

      The Plant Journal

      Volume: - Issue: 2 Pages: 563-576

    • DOI

      10.1111/tpj.16245

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Involvement of the Membrane Trafficking Factor PATROL1 in the Salinity Stress Tolerance of <i>Arabidopsis thaliana</i>2021

    • Author(s)
      Sato F, Iba K, Higaki T
    • Journal Title

      CYTOLOGIA

      Volume: 86 Issue: 2 Pages: 119-126

    • DOI

      10.1508/cytologia.86.119

    • NAID

      130008056667

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2021-06-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Environmental Responses of the Membrane Trafficking Factor PATROL1 in the Arabidopsis Stomatal Complex2021

    • Author(s)
      Higaki, T., Sato, F. and Iba, K.
    • Journal Title

      CYTOLOGIA

      Volume: 86 Issue: 2 Pages: 101-102

    • DOI

      10.1508/cytologia.86.102

    • NAID

      130008056691

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2021-06-25
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cuticle permeability is an important parameter for the trade-off strategy between drought tolerance and CO2 uptake in land plants2021

    • Author(s)
      Monda Keina、Mabuchi Atsushi、Negi Juntaro、Iba Koh
    • Journal Title

      Plant Signaling & Behavior

      Volume: 16 Issue: 6 Pages: 1908692-1908692

    • DOI

      10.1080/15592324.2021.1908692

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Endoplasmic Reticulum Pathway for Membrane Lipid Synthesis Has a Significant Contribution toward Shoot Removal-Induced Root Chloroplast Development in Arabidopsis2021

    • Author(s)
      Obata Tomoki、Kobayashi Koichi、Tadakuma Ryosuke、Akasaka Taiki、Iba Koh、Negi Juntaro
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 3 Pages: 494-501

    • DOI

      10.1093/pcp/pcab009

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced NRT1.1/NPF6.3 expression in shoots improves growth under nitrogen deficiency stress in Arabidopsis2021

    • Author(s)
      Sakuraba Yasuhito、Chaganzhana、Mabuchi Atsushi、Iba Koh、Yanagisawa Shuichi
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1 Pages: 256-256

    • DOI

      10.1038/s42003-021-01775-1

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 気孔細胞に存在する葉緑体の成り立ちとその機能2021

    • Author(s)
      祢冝 淳太郎、小畑 智暉、宋 普錫
    • Journal Title

      BSJ-Review

      Volume: 12 Issue: A Pages: 38-44

    • DOI

      10.24480/bsj-review.12a5.00198

    • ISSN
      2432-9819
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increased Cuticle Permeability Caused by a New Allele of ACETYL-COA CARBOXYLASE1 Enhances CO2 Uptake2020

    • Author(s)
      Monda Keina、Mabuchi Atsushi、Takahashi Sho、Negi Juntaro、Tohmori Ryoma、Terashima Ichiro、Yamori Wataru、Iba Koh
    • Journal Title

      Plant Physiology

      Volume: 184 Issue: 4 Pages: 1917-1926

    • DOI

      10.1104/pp.20.00978

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improved stomatal opening enhances photosynthetic rate and biomass production in fluctuating light2020

    • Author(s)
      Haruki Kimura, Mimi Hashimoto-Sugimoto, Koh Iba, Ichiro Terashima, Wataru Yamori
    • Journal Title

      J Experimental Botany

      Volume: 71 Issue: 7 Pages: 2339-2350

    • DOI

      10.1093/jxb/eraa090

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased stomatal conductance induces rapid changes to photosynthetic rate in response to naturally fluctuating light conditions in rice.2020

    • Author(s)
      Yamori, W., Kusumi, K., Iba, K. and Terashima, I.
    • Journal Title

      Plant, Cell and Environment

      Volume: in press Issue: 5 Pages: 1-11

    • DOI

      10.1111/pce.13725

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] 塩ストレスにより表皮細胞特異的に誘導されるアントシアニンの蓄積には表皮葉緑体が必要である2024

    • Author(s)
      小畑 智暉、立花 輝太、王丸 将貴、射場 厚、袮冝 淳太郎
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] アブシシン酸非依存的な気孔応答に関係するシロイヌナズナ新規変異体の解析2023

    • Author(s)
      中江 聡子、秀野 智紀、祢冝 淳太郎、小嶋 美紀子、竹林 裕美子、榊原 均、射場 厚、門田 慧奈
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 脂質合成酵素に異常を持つ新規気孔葉緑体形成不全変異体の単離及びリピドーム解析2023

    • Author(s)
      大隈 玲央奈、宋 普錫、山柿 将、小畑 智暉、西村 さくら、射場 厚、祢冝 淳太郎
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] シロイヌナズナの気孔無傷葉緑体単離法の確立と気孔葉緑体特異的タンパク質の探索2022

    • Author(s)
      宋 普錫, 山柿 将, 西村 さくら, 成松 丈, 射場 厚, 祢冝 淳太郎
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 根細胞において小胞体経路は主要な膜脂質合成経路であり葉緑体の発達誘導に欠かせない2022

    • Author(s)
      小畑 智暉、小林 康一、多田隈 遼亮、赤坂 泰輝、射場 厚、袮冝 淳太郎
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] アブシシン酸非依存的にCO2誘導性の気孔閉鎖能が低下した新規変異体の単離2022

    • Author(s)
      中江 聡子, 秀野 智紀, 東馬場 徳, 馬渕 敦士, 祢冝 淳太郎, 射場 厚, 門田 慧奈
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] CO2高感受性を示すシロイヌナズナ野生系統の単離とその表現型解析2022

    • Author(s)
      秀野 智紀, 中江 聡子, 東馬場 徳, 馬渕 敦士, 祢冝 淳太郎, 射場 厚, 門田 慧奈
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] シロイヌズナsORF, SORFC03は全身的な窒素シグナルに応じて発現し側根形成を抑制する2022

    • Author(s)
      伊藤 和洋, 山本 あゆ, 馬渕 敦士, 花田 耕介, 射場 厚, 楠見 健介
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 気孔機能化因子SCAP1の気孔特異的な発現に必要な2つの独立した新規シスエレメントの同定2021

    • Author(s)
      森脇 宏介, 柳澤 修一, 射場 厚, 祢冝 淳太郎
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] シロイヌナズナの気孔葉緑体および気孔開閉応答には葉緑体脂質DGDGが重要である2021

    • Author(s)
      西村 さくら, 小畑 智暉, 多田隈 遼亮, 射場 厚, 袮冝 淳太郎
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Identification of two independent cis-acting elements required for the guard cell-specific expression of SCAP1, which is essential for late stomatal development2021

    • Author(s)
      Kosuke Moriwaki, Shuichi Yanagisawa, Koh Iba, Juntaro Negi
    • Organizer
      The 31st International Conference on Arabidopsis Research (ICAR2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The ER pathway for membrane lipid synthesis has an important role in shoot removal-induced root chloroplast development in Arabidopsis2021

    • Author(s)
      Tomoki Obata, Ryosuke Tadakuma, Taiki Akasaka, Koichi Kobayashi, Koh Iba, Juntaro Negi
    • Organizer
      The 31st International Conference on Arabidopsis Research (ICAR2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 気孔成熟化プロセスの鍵転写因子SCAP1の気孔特異的な発現に必要な2つの新規シスエレメント2021

    • Author(s)
      森脇 宏介,柳澤 修一,射場 厚,祢冝淳太郎
    • Organizer
      九州沖縄植物学会第70回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 葉緑体脂質DGDGは気孔葉緑体形成及び気孔開閉応答に必須である2021

    • Author(s)
      西村 さくら, 小畑 智暉, 多田隈 遼亮, 射場 厚, 袮冝 淳太郎
    • Organizer
      九州沖縄植物学会第70回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Shoot-to-root long-distance signals found in an Arabidopsis thaliana ecotype improve growth under nitrogen deficient conditions2021

    • Author(s)
      Mabuchi A, Monda K, Notaguchi M, Tsutsui H, Sakuraba Y, Negi J, Abe M, Yanagisawa S, Iba K
    • Organizer
      The 31st International Conference on Arabidopsis Research (ICAR2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Increased Cuticle Permeability Caused by a New Allele of ACETYL-COA CARBOXYLASE 1 Enhances CO2 Uptake Efficiency in Arabidopsis2021

    • Author(s)
      Monda K, Mabuchi A, Takahashi S, Negi J, Tohmori R, Terashima I, Yamori W, Iba K
    • Organizer
      The 31st International Conference on Arabidopsis Research (ICAR2021)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロイヌナスズナ野生系統がもつ極限低窒素環境適応力を高める移動性シグナル2021

    • Author(s)
      馬渕 敦士, 門田 慧奈, 野田口 理孝, 筒井 大貴, 櫻庭 康仁, 祢冝 淳太郎, 阿部 光知, 柳澤 修一, 射場 厚
    • Organizer
      九州沖縄植物学会第70回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ACETYL-COA CARBOXYLASE 1の新規変異によるクチクラ透過性の上昇はCO2取込み効率を向上させる2021

    • Author(s)
      門田 慧奈, 馬渕 敦士, 高橋 將, 袮冝 淳太郎, 東森 崚馬, 寺島 一郎, 矢守 航, 射場 厚
    • Organizer
      九州沖縄植物学会第70回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 側根形成の窒素栄養応答に関わるシロイヌナズナshort ORFの機能解析2021

    • Author(s)
      伊藤 和洋, 久永 優希, 馬渕 敦士, 花田 耕介, 射場 厚, 楠見 健介
    • Organizer
      日本植物学会 第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 高窒素環境における側根形成調節に関わるシロイヌナズナshort ORFの機能解析2021

    • Author(s)
      久永 優希, 伊藤 和洋, 馬渕 敦士, 花田 耕介, 射場 厚, 楠見 健介
    • Organizer
      九州沖縄植物学会(第70回)
    • Related Report
      2021 Annual Research Report
  • [Presentation] An Arabidopsis short ORF, SORFC03, is involved in the regulation of nitrogen-dependent lateral root developmen2021

    • Author(s)
      Kazuhiro Ito, Yuki Hisanaga, Kousuke Hanada, Koh Iba, Kensuke Kusumi
    • Organizer
      The International Conference on Arabidopsis Research (ICAR)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 地上部切除により誘導される根細胞での葉緑体の発達には小胞体からの脂質供給が欠かせない2021

    • Author(s)
      小畑 智暉、小林 康一、多田隈 遼亮、赤坂 泰輝、射場 厚、袮冝 淳太郎
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 気孔の成熟化を担うDof転写因子SCAP1の気孔特異的な発現に必要な2つの新規シスエレメント2021

    • Author(s)
      森脇 宏介, 柳澤 修一, 射場 厚, 祢冝 淳太郎
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナのACETYL-COA CARBOXYLASE1の新規変異が引き起こすクチクラ透過性の上昇は,CO2取り込み効率を向上させる2021

    • Author(s)
      門田 慧奈, 馬渕 敦士, 高橋 將, 袮冝 淳太郎, 東森 崚馬, 寺島 一郎, 矢守 航, 射場 厚
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 窒素栄養環境に応じた側根形成調節に関わるシロイヌナズナshort ORFの解析2021

    • Author(s)
      伊藤 和洋, 山本 あゆ, 馬渕 敦士, 花田 耕介, 射場 厚, 楠見 健介
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Dominant role of ER lipid pathway for shoot-removal induced root chloroplast development in Arabidopsis2020

    • Author(s)
      Tomoki Obata, Ryosuke Tadakuma, Taiki Akasaka, Koichi Kobayashi, Koh Iba, Juntaro Negi
    • Organizer
      The Plant Biology 2020 Worldwide Summit
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 極限低窒素適応形質の接木によるシロイヌナズナエコタイプ間移動2020

    • Author(s)
      馬渕 敦士, 門田 慧奈, 野田口 理孝, 筒井 大貴, 櫻庭 康仁, 祢冝 淳太郎, 阿部 光知, 柳澤 修一, 射場 厚
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネの葉の発生初期におけるppGpp合成を介した葉緑体分化制御2020

    • Author(s)
      伊藤和洋, 伊藤道俊, 増田真二, 射場厚, 楠見健介
    • Organizer
      日本植物学会 第84回大会
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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