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A study of plant polyspermy using Arabidopsis mutants detective in the synergid-endosperm fusion.

Research Project

Project/Area Number 20H03280
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionYokohama City University

Principal Investigator

Maruyama Daisuke  横浜市立大学, 木原生物学研究所, 准教授 (80724111)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2022: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2021: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2020: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Keywords花粉管 / 助細胞 / 細胞融合 / 多精 / 被子植物 / 重複受精 / 卵細胞 / 胚乳 / 多花粉管拒否 / 多精拒否 / 中央細胞 / 助細胞胚乳融合 / シロイヌナズナ
Outline of Research at the Start

卵に複数の精子が受精しないように制御する多精拒否のしくみは,生物に広く保存されている.被子植物の場合,卵細胞の隣にある2つの助細胞が,精細胞の運び手である花粉管の誘引を,受精後すぐに停止することで多精のリスクを減らしている.その誘引停止に伴う助細胞の形態変化として,我々は助細胞と胚乳が融合する現象を発見した.この助細胞胚乳融合は,精細胞の放出先を受精不可能な領域へと変更する「切り替えスイッチ」として働くことで多精拒否に貢献する可能性がある.本研究では助細胞胚乳融合の変異体の解析を通じ,被子植物の多精拒否に助細胞胚乳融合がはたす役割を明らかにする.

Outline of Final Research Achievements

In flowering plants, immotile two sperm cells are delivered by a pollen tube and finally released into one of the two synergid cells secreting pollen tube attractant peptides in the ovule. During pollen tube discharge, sperm nuclei enter the egg cell and the central cell by double fertilization, while the other pollen tube contents (PTC) remain outside of those female gametes. However, we found unusual PTC entry named DEAD-End in fertilized central cell through the observation of thousands of ovules. Non-receptive persistent synergid is absorbed by early endosperm in fertilized ovules (SE fusion). DEAD-End can be caused by reception of second pollen tube by the inactivating persistent synergid after SE fusion. We identified a key factor of SE fusion and demonstrated its prerequisite role in DEAD-End. These results indicate novel polyspermy blocking mechanism in flowering plants and provide molecular insight of unique cell fusion mechanism and morphological plasticity in plant cells.

Academic Significance and Societal Importance of the Research Achievements

動物の生殖では、卵に対して多くの精子が同時にアクセスするため、雌雄の配偶子が1対1で受精できるように保証する多精拒否のメカニズムが存在する。被子植物にも同様の仕組みが存在することが示唆されていたが、実態は明らかとなっていない。本研究によって植物の多精拒否のメカニズムの一端が明らかになることで、生物の多様な生殖機構の理解に貢献するとともに、多精を利用した新規の育種技術の開発につながると期待される。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (16 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (6 results) (of which Invited: 1 results) Remarks (1 results)

  • [Int'l Joint Research] ケンタッキー大学(米国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] ケンタッキー大学(米国)

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Transcriptome analyses uncover reliance of endosperm gene expression on Arabidopsis embryonic development2023

    • Author(s)
      Zhang Yilin、Maruyama Daisuke、Toda Erika、Kinoshita Atsuko、Okamoto Takashi、Mitsuda Nobutaka、Takasaki Hironori、Ohme‐Takagi Masaru
    • Journal Title

      FEBS Letters

      Volume: 597 Issue: 3 Pages: 407-417

    • DOI

      10.1002/1873-3468.14570

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Exploring Novel Polytubey Reproduction Pathways Utilizing Cumulative Genetic Tools2023

    • Author(s)
      Sugi Naoya、Maruyama Daisuke
    • Journal Title

      Plant and Cell Physiology

      Volume: - Issue: 5 Pages: 454-460

    • DOI

      10.1093/pcp/pcad021

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Elongation of Siliques Without Pollination 3 Regulates Nutrient Flow Necessary for Embryogenesis2022

    • Author(s)
      Takasaki Hironori、Ikeda Miho、Hasegawa Reika、Zhang Yilin、Sakamoto Shingo、Maruyama Daisuke、Mitsuda Nobutaka、Kinoshita Tetsu、Ohme-Takagi Masaru
    • Journal Title

      Plant and Cell Physiology

      Volume: 64 Issue: 1 Pages: 117-123

    • DOI

      10.1093/pcp/pcac151

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] F-actin regulates the polarized secretion of pollen tube attractants in Arabidopsis synergid cells2022

    • Author(s)
      Susaki Daichi、Izumi Rie、Oi Takao、Takeuchi Hidenori、Shin Ji Min、Sugi Naoya、Kinoshita Tetsu、Higashiyama Tetsuya、Kawashima Tomokazu、Maruyama Daisuke
    • Journal Title

      The Plant Cell

      Volume: 35 Issue: 4 Pages: 1222-1240

    • DOI

      10.1093/plcell/koac371

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Persistent directional growth capability in Arabidopsis thaliana pollen tubes after nuclear elimination from the apex2021

    • Author(s)
      Motomura Kazuki、Takeuchi Hidenori、Notaguchi Michitaka、Tsuchi Haruna、Takeda Atsushi、Kinoshita Tetsu、Higashiyama Tetsuya、Maruyama Daisuke
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 2331-2331

    • DOI

      10.1038/s41467-021-22661-8

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis2021

    • Author(s)
      Susaki Daichi、Suzuki Takamasa、Maruyama Daisuke、Ueda Minako、Higashiyama Tetsuya、Kurihara Daisuke
    • Journal Title

      PLOS Biology

      Volume: 19 Issue: 3 Pages: 3001123-3001123

    • DOI

      10.1371/journal.pbio.3001123

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arabidopsis GEX1 Is a Nuclear Membrane Protein of Gametes Required for Nuclear Fusion During Reproduction2020

    • Author(s)
      Nishikawa Shuh-ichi、Yamaguchi Yuki、Suzuki Chiharu、Yabe Ayaka、Sato Yuzuru、Kurihara Daisuke、Sato Yoshikatsu、Susaki Daichi、Higashiyama Tetsuya、Maruyama Daisuke
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 548032-548032

    • DOI

      10.3389/fpls.2020.548032

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 植物受精の動態をライブイメージングで観察する2023

    • Author(s)
      丸山大輔
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナの非配偶子性の細胞融合の意義を理解する2022

    • Author(s)
      丸山大輔
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 受精依存的に発現する シロイヌナズナの細胞融合因子の同定2021

    • Author(s)
      丸山大輔
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] シロイヌナズナの生殖に伴う非配偶子融合の意義2021

    • Author(s)
      丸山 大輔,須﨑 大地,太田 かおる,木下 哲
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 死を回避した助細胞は新たな運命を手に入れられるか2021

    • Author(s)
      丸山大輔
    • Organizer
      第62回日本植物生理学会年会・関連集会「植物生殖改変ワークショップ」
    • Related Report
      2020 Annual Research Report
  • [Presentation] 助細胞の細胞融合現象に異常を示すシロイヌナズナ変異体の解析2021

    • Author(s)
      丸山大輔、太田かおる、須﨑大地、木下哲
    • Organizer
      日本植物学会第 84回大会
    • Related Report
      2020 Annual Research Report
  • [Remarks] アクチン繊維が花粉管の誘引を制御する ~助細胞による誘引ペプチド分泌のメカニズムを解明~

    • URL

      https://www.yokohama-cu.ac.jp/news/2022/202301susaki.html

    • Related Report
      2022 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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