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Molecular basis of semilunar spawning rhythm: Involvement of a tidal cycle-related biological clock.

Research Project

Project/Area Number 20H03288
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44040:Morphology and anatomical structure-related
Research InstitutionNiigata University

Principal Investigator

Ando Hironori  新潟大学, 佐渡自然共生科学センター, 教授 (60221743)

Co-Investigator(Kenkyū-buntansha) 豊田 賢治  金沢大学, 環日本海域環境研究センター, 特任助教 (00757370)
吉村 崇  名古屋大学, 生命農学研究科(WPI), 教授 (40291413)
大森 紹仁  新潟大学, 佐渡自然共生科学センター, 助教 (50613527)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2023: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Keywords生殖リズム / 潮汐サイクル / 月周リズム / 体内時計 / 概潮汐時計 / 生殖神経内分泌系 / メラトニン / 松果体 / 半月周リズム
Outline of Research at the Start

海洋生物の生殖や生態は、月齢や潮汐に同調したリズムを持つものが多い。そのリズムは、脳内にある体内時計によって作られるが、そのしくみは不明である。クサフグは、新月と満月の日の満潮前に産卵を繰り返す。これまでの研究から、脳内に概潮汐時計が存在し、概日時計と協同して半月周性産卵リズムを作ることが示唆された。本研究では、概潮汐時計の分子基盤を解明するため、潮汐と月齢に同調して発現する遺伝子群を探索すると共に、それらの潮汐サイクルとの同調および半月周性リズムとの関連を検討する。また、半月周性リズム発振機構がどのようなしくみで生殖機能を調節するのか、さらに、発生や成長の段階でいつ形成されるのかを解明する。

Outline of Final Research Achievements

In the pineal gland, it was estimated that approximately 1000 genes fluctuate in expression on a semidiurnal cycle, supporting the idea that the circatidal clock works in conjunction with the circadian clock to form a semilunar spawning rhythm. The male grass puffer from Sado Island, where there is little tidal variation, do not show lunar age-dependent gene expression, suggesting that they do not have the mechanism for regulating the semilunar spawning rhythm. We identified the transcription factor C/EBPd, which regulates semilunar gene expression, as one of the cyclic regulatory mechanisms of the reproductive neuroendocrine system. It was also suggested that melatonin is involved in the oscillation of gene expression synchronized with the tidal cycle and regulates the reproductive neuroendocrine system cyclically. The locomotion of larval and juvenile fish exhibited a circadian rhythm under constant darkness as well as a bimodal rhythm in response to light stimulation.

Academic Significance and Societal Importance of the Research Achievements

生物はそれぞれの生息環境に合わせて独自の生殖リズムを持っている。中でも多くの海洋生物は月齢や潮汐サイクルに同調した生殖リズムを持っているが、そのリズムを調節する分子機構は不明である。本研究では、大潮の満潮前に産卵するクサフグをモデルとして、生殖神経内分泌系の周期的調節という点から研究を行い、潮汐サイクルに同調する体内時計の機能的分子基盤の一端を明らかにした。本研究は、これまで概日時計の分子機構に焦点があてられてきた時間生物学の方向を転換させて、生物が持つ多様な体内時計機構を解明する研究であり、生物リズムに関するより普遍的な生物原理の発見へとつながるものとして、その学術的また社会的意義は大きい。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (50 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (4 results) Journal Article (14 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 14 results,  Open Access: 7 results) Presentation (29 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Book (2 results) Remarks (1 results)

  • [Int'l Joint Research] バングラデシュ農業大学/チャットグラム獣医動物科学大学(バングラデシュ)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] バングラデシュ農業大学/チャットグラム獣医動物科学大学(バングラデシュ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] バングラデシュ農業大学/チャットグラム獣医動物大学(バングラデシュ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] チャットグラム獣医動物科学大学(バングラデシュ)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Taxonomic status of the oreasterid sea star Bothriaster primigenius Döderlein, 1916 (Echinodermata, Asteroidea)2023

    • Author(s)
      Kogure, Y., Omori, A., Fujita, Y.
    • Journal Title

      Biogeography

      Volume: 25 Issue: 0 Pages: 13-18

    • DOI

      10.11358/biogeo.25.13

    • ISSN
      1345-0662, 1880-8085
    • Year and Date
      2023-09-20
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lunar Age-Dependent Oscillations in Expression of the Genes for Kisspeptin, GnIH, and Their Receptors in the Grass Puffer during the Spawning Season2023

    • Author(s)
      Shahjahan Md., Rahman Mohammad Lutfar, Ohno Yuki, Zahangir Md. Mahiuddin, Ando Hironori
    • Journal Title

      Zoological Science

      Volume: 41 Issue: 1 Pages: 97-104

    • DOI

      10.2108/zs230061

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Eyestalk transcriptome and methyl farnesoate titers provide insight into the physiological changes in the male snow crab, Chionoecetes opilio, after its terminal molt2023

    • Author(s)
      Toyota Kenji、Yamamoto Takeo、Mori Tomoko、Mekuchi Miyuki、Miyagawa Shinichi、Ihara Masaru、Shigenobu Shuji、Ohira Tsuyoshi
    • Journal Title

      Scientific Reports

      Volume: 13 Issue: 1 Pages: 7204-7204

    • DOI

      10.1038/s41598-023-34159-y

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anomalous Temperature Interdicts the Reproductive Activity in Fish: Neuroendocrine Mechanisms of Reproductive Function in Response to Water Temperature2022

    • Author(s)
      Zahangir Md. Mahiuddin、Rahman Mohammad Lutfar、Ando Hironori
    • Journal Title

      Frontiers in Physiology

      Volume: 13 Pages: 902257-902257

    • DOI

      10.3389/fphys.2022.902257

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Kisspeptin Exhibits Stimulatory Effects on Expression of the Genes for Kisspeptin Receptor, GnRH1 and GTH Subunits in a Gonadal Stage-Dependent Manner in the Grass Puffer, a Semilunar-Synchronized Spawner2022

    • Author(s)
      Zahangir Md. Mahiuddin、Shahjahan Md.、Ando Hironori
    • Journal Title

      Frontiers in Endocrinology

      Volume: 13

    • DOI

      10.3389/fendo.2022.917258

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prostaglandin E2 synchronizes lunar-regulated beach spawning in grass puffers2022

    • Author(s)
      Chen Junfeng、Katada Yuma、Okimura Kousuke、Yamaguchi Taiki、Guh Ying-Jey、Nakayama Tomoya、Maruyama Michiyo、Furukawa Yuko、Nakane Yusuke、Yamamoto Naoyuki、Sato Yoshikatsu、Ando Hironori、Sugimura Asako、Tabata Kazufumi、Sato Ayato、Yoshimura Takashi
    • Journal Title

      Current Biology

      Volume: 32 Issue: 22 Pages: 4881-4889

    • DOI

      10.1016/j.cub.2022.09.062

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The pentameric hydrocoel lobes organize adult pentameral structures in a sea cucumber, Apostichopus japonicus2022

    • Author(s)
      Udagawa Sumio、Nagai Akiko、Kikuchi Mani、Omori Akihito、Tajika Atsushi、Saito Mieko、Miura Toru、Irie Naoki、Kamei Yasuhiro、Kondo Mariko
    • Journal Title

      Developmental Biology

      Volume: 492 Pages: 71-78

    • DOI

      10.1016/j.ydbio.2022.09.002

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Laterally biased diffusion of males of the water flea Daphnia magna2022

    • Author(s)
      Toyota Kenji、Yasugi Masaki、Tatarazako Norihisa、Iguchi Taisen、Watanabe Eiji
    • Journal Title

      Journal of Experimental Zoology Part A: Ecological and Integrative Physiology

      Volume: 337 Issue: 6 Pages: 626-638

    • DOI

      10.1002/jez.2595

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Juvenile hormone synthesis and signaling disruption triggering male offspring induction and population decline in cladocerans (water flea): Review and adverse outcome pathway development2022

    • Author(s)
      Toyota Kenji、Watanabe Haruna、Hirano Masashi、Abe Ryoko、Miyakawa Hitoshi、Song You、Sato Tomomi、Miyagawa Shinichi、Tollefsen Knut Erik、Yamamoto Hiroshi、Tatarazako Norihisa、Iguchi Taisen
    • Journal Title

      Aquatic Toxicology

      Volume: 243 Pages: 106058-106058

    • DOI

      10.1016/j.aquatox.2021.106058

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of blue light on the growth of the red swamp cray fish Procambraus clarkii larvae -seasonal and sexual differences2022

    • Author(s)
      Kenji Toyota, Kazuki Usami, Kanta Mizusawa, Tsuyoshi Ohira
    • Journal Title

      Zoological Studies

      Volume: 61 Pages: 3-3

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Light-induced and circadian expressions of melanopsin genes opn4xa and opn4xb in the eyes of juvenile grass puffer Takifugu alboplumbeus2021

    • Author(s)
      Kitahashi Takashi、Kurokawa Daisuke、Ogiso Shouzo、Suzuki Nobuo、Ando Hironori
    • Journal Title

      Fish Physiology and Biochemistry

      Volume: 47 Issue: 2 Pages: 191-202

    • DOI

      10.1007/s10695-020-00901-w

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Higher acclimation temperature affects growth of rohu (Labeo rohita) through suppression of GH and IGFs genes expression actuating stress response2021

    • Author(s)
      Shahjahan Md、Zahangir Md. Mahiuddin、Islam S.M. Majharul、Ashaf-Ud-Doulah Mohammad、Ando Hironori
    • Journal Title

      Journal of Thermal Biology

      Volume: 100 Pages: 103032-103032

    • DOI

      10.1016/j.jtherbio.2021.103032

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Expression dynamics of the genes for the hypothalamo-pituitary-gonadal axis in tiger puffer (Takifugu rubripes) at different reproductive stages2021

    • Author(s)
      Zahangir Md. Mahiuddin、Matsubara Hajime、Ogiso Shouzo、Suzuki Nobuo、Ueda Hiroshi、Ando Hironori
    • Journal Title

      General and Comparative Endocrinology

      Volume: 301 Pages: 113660-113660

    • DOI

      10.1016/j.ygcen.2020.113660

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Gene expression analysis of three homeobox genes throughout early and late development of a feather star Anneissia japonica2020

    • Author(s)
      Omori Akihito、Shibata Tomoko F.、Akasaka Koji
    • Journal Title

      Development Genes and Evolution

      Volume: 230 Issue: 4 Pages: 305-314

    • DOI

      10.1007/s00427-020-00665-6

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] 月周産卵魚クサフグにおけるメラトニン受容体(Mel1b)遺伝子の発現細胞の脳内分布2023

    • Author(s)
      豊増唯、大森紹仁、服部淳彦、安東宏徳
    • Organizer
      日本動物学会第94回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 月のリズムと季節のリズムの分子基盤2023

    • Author(s)
      吉村崇
    • Organizer
      下垂体研究会第37回学術集会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Understanding the mystery of biological clocks: Learning from unique animals to contribute for food production and human health2023

    • Author(s)
      Yoshimura T
    • Organizer
      JAACT2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ウミシダにおける間充織細胞の発生と分化2023

    • Author(s)
      長部 有沙、大森 紹仁
    • Organizer
      日本動物学会第94回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Comparative Crustacean Biology-Application for aquaculture and ecophysiology2023

    • Author(s)
      Kenji Toyota
    • Organizer
      The Philippine Society for Developmental Biology, 15th Annual Convention
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] アカテガニの月周期依存的な繁殖生理機構2023

    • Author(s)
      豊田賢治
    • Organizer
      日本動物学会中部支部大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] アカテガニの月周繁殖リズムの生理機構2023

    • Author(s)
      豊田賢治、 片山秀和、 大平 剛
    • Organizer
      日本甲殻類学会第 61 回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] アカテガニの月周繁殖リズムの生理機構2023

    • Author(s)
      豊田賢治
    • Organizer
      日本睡眠学会第45回定期学術集会・第30回日本時間生物学会学術大会合同大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 月周同調産卵魚クサフグにおける生殖神経内分泌系の周期的調節2022

    • Author(s)
      安東宏徳
    • Organizer
      第36回日本下垂体研究会学術集会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] クサフグ松果体における半日周発現遺伝子の探索2022

    • Author(s)
      Md. Mahiuddin Zahangir,大野佑紀,豊田賢治,安東宏徳
    • Organizer
      第46回日本比較内分泌学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] クサフグの半月周リズムに関する研究2022

    • Author(s)
      片田祐真,Chen Junfeng,沖村光祐,山口大輝,Guh Ying-Jey,中山友哉,丸山迪代,古川祐子,中根右介,山本直之,佐藤良勝,安東宏徳,杉村麻子,田畑和文,佐藤綾人,吉村崇
    • Organizer
      第46回日本比較内分泌学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Prostaglandin E2 synchronizes lunar-regulated beach-spawning in grass puffers.2022

    • Author(s)
      Junfeng Chen, Yuma Katada, Kousuke Okimura, Taiki Yamaguchi, Ying-Jey Guh, Tomoya Nakayama, Michiyo Maruyama, Yuko Furukawa, Yusuke Nakane, Naoyuki Yamamoto, Yoshikatsu Sato, Hironori Ando, Asako Sugimura, Kazufumi Tabata, Ayato Sato, Takashi Yoshimura
    • Organizer
      Sapporo Symposium on Biological Rhythm 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Prostaglandin E2 synchronizes lunar-regulated beach-spawning in grass puffers2022

    • Author(s)
      山口大輝,片田祐真,Chen Junfeng,沖村光祐,Guh Ying-Jey,中山友哉,丸山迪代,古川祐子,中根右介,山本直之,安東宏徳,杉村麻子,田畑和文,佐藤良勝,佐藤綾人,吉村崇
    • Organizer
      第29回日本時間生物学会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 佐渡島の砂泥海岸におけるサドナデシコナマコの微小棲息環境2022

    • Author(s)
      梅田樹,大森紹仁
    • Organizer
      日本動物学会第93回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 甲殻類における幼若ホルモンと眼柄ホルモンの生理機能解明に関する研究2022

    • Author(s)
      豊田賢治
    • Organizer
      第46回日本比較内分泌学会大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] アカテガニの月周性繁殖リズム2022

    • Author(s)
      豊田賢治
    • Organizer
      日本動物学会第93回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] クサフグ前脳における半月周発現遺伝子群:神経ペプチド前駆体遺伝子群と受容体遺伝子群の発現変動パターンの逆相関2021

    • Author(s)
      安東宏徳,Md. Mahiuddin Zahangir,豊田賢治
    • Organizer
      日本動物学会第92回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] クサフグの半月周リズムに関する研究2021

    • Author(s)
      片田祐真, Chen Junfeng, 沖村光祐, Guh Ying-Jey, 中山友哉, 丸山迪代, 山口大輝, 安東宏徳, 佐藤綾人, 吉村崇
    • Organizer
      第28回日本時間生物学会学術大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] アカテガニの半月周性繁殖リズムの生理機構の理解に向けて2021

    • Author(s)
      豊田賢治,近藤裕介,鈴木信雄,大平剛,安東宏徳
    • Organizer
      第59回日本甲殻類学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] クサフグ仔稚魚における自発行動リズムの解析2021

    • Author(s)
      江野脩太,片田祐真,黒川大輔,豊田賢治,飯田碧,吉村崇,安東宏徳
    • Organizer
      第45回日本比較内分泌学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 佐渡島におけるアカテガニの半月周性繁殖リズム2021

    • Author(s)
      豊田賢治,大平剛,安東宏徳
    • Organizer
      第45回日本比較内分泌学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] RNA-seqで探るサケの産卵回遊(母川回帰)の分子神経内分泌機構2021

    • Author(s)
      浦野明央,小沼健,北橋隆史,安東宏徳,福若雅章,伴真俊,兵藤晋
    • Organizer
      第45回日本比較内分泌学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 水族館就職希望の専門学校生を対象としたフィールドワークの実践2021

    • Author(s)
      鈴木倫明,安東宏徳,前田英雅,若林耕治
    • Organizer
      東京大学大気海洋研究所研究集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] クサフグの半月周リズムに関するトランスクリプトーム解析2021

    • Author(s)
      片田祐真,Chen Junfeng,沖村光祐,Guh Ying-Jey,中山友哉,丸山迪代,安東宏徳,吉村崇
    • Organizer
      令和4年度日本水産学会春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 佐渡島沿岸におけるイボカギナマコの分布及び棲息環境の解明2021

    • Author(s)
      梅田樹,中島崇志,大森紹仁
    • Organizer
      第17回棘皮動物研究集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 幼若ホルモンがズワイガニのオスらしさを決める?2021

    • Author(s)
      豊田賢治, 山本岳男, 宮川信一, 井原賢, 森友子, 重信秀治, 大平剛
    • Organizer
      第92回日本動物学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Dim light at night stimulates melatonin production and may influence the expression of the GnRH, GnIH, and kisspeptin genes in the grass puffer2020

    • Author(s)
      Md. Mahiuddin Zahangir、Weronika Palkowska、丸山雄介、服部淳彦、安東宏徳
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヌタウナギにおける2種類の下垂体糖タンパク質ホルモン受容体をコードする候補遺伝子の同定2020

    • Author(s)
      村松万里,小林哲也,内田勝久,安東宏徳,持田弘,岡田令子,鈴木雅一
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] サドナデシコナマコの分布と生殖生態2020

    • Author(s)
      中島崇志,大森紹仁
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2020 Annual Research Report
  • [Book] Handbook of Hormones-Comparative Endocrinology for Basic and Clinical Research, 2nd edition2021

    • Author(s)
      Hironori Ando, Kazuyoshi Ukena, Shinji Nagata
    • Total Pages
      1113
    • Publisher
      Academic Press
    • ISBN
      9780128206492
    • Related Report
      2021 Annual Research Report
  • [Book] Japanese Marine Life: A Practical Training Guide in Marine Biology2020

    • Author(s)
      Yukitoshi Katayama, Takashi Kitahashi, Yukitoshi Katayama, Takashi Kitahashi, Nobuo Suzuki, Tatsuya Sakamoto
    • Total Pages
      383
    • Publisher
      Springer
    • ISBN
      9789811513251
    • Related Report
      2020 Annual Research Report
  • [Remarks] 新潟大学佐渡自然共生科学センター臨海実験所

    • URL

      https://www.sc.niigata-u.ac.jp/sc/sadomarine/

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report 2020 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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