• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Evolutionary biological studies of initial transitions of gender phenotypes

Research Project

Project/Area Number 20H03299
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 45020:Evolutionary biology-related
Research InstitutionThe University of Tokyo

Principal Investigator

Nozaki Hisayoshi  東京大学, 大学院理学系研究科(理学部), 客員共同研究員 (40250104)

Co-Investigator(Kenkyū-buntansha) 山口 晴代  国立研究開発法人国立環境研究所, 生物多様性領域, 主任研究員 (20722672)
河地 正伸  国立研究開発法人国立環境研究所, 生物多様性領域, 室長 (50260469)
高橋 文雄  東邦大学, 薬学部, 講師 (60332318)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2021: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2020: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords性進化 / 性多様性 / 性決定遺伝子 / 性染色体 / 性決定領域 / ボルボックス類 / 全ゲノム解析 / 両性型 / 緑藻ボルボックス / 雌雄異体種 / バイセクシュアル種 / 進化 / 交配システムの進化 / ホモタリック / ヘテロタリック / 緑藻類 / ボルボックス系列 / プレオドリナ / ボルボックス / 交配システム転換進化 / 性表現進化 / 雌雄性 / ゲノム / 緑藻ボルボックス系列 / 交配システム進化 / Volvox / 性決定領域(SDR)
Outline of Research at the Start

メスとオスの配偶子が同一個体につくられる雌雄同体の自殖種の原型は微細藻類・菌類に認められ、“ヘテロタリック(雌雄異株)”の性表現とは別に、一つの株内に両性の配偶子をつくる”ホモタリック(雌雄同株)”が存在する。本研究では性の進化生物学的研究が進展している「緑藻ボルボックス系列」に着目する。特に、メス・オスに加えてホモタリックの3 種類の性表現をもつホモタリック種への移行段階のプレオドリナの1種、並びにホモタリック種からヘテロタリック種への進化の初期段階に相当することが推測されるボルボックスの1種を材料として、ホモタリック種とヘテロタリック種の相互転換の初期進化の分子遺伝学的基盤を明らかにする。

Outline of Final Research Achievements

Whole genomes of closely related species of Volvox, heterothallic V.reticuliferus and homothallic V. africanus, were generated and analyzed to reveal expanded (ca. 1 Mbp.) sex-determining regions (SDRs) in V. reticuliferus and expanded (ca. 1 Mbp.) SDR-like region (SDLR)in V. africanus. SDLRs were continuous and had male or female origin. Thus, expanded SDR/SDLR seems essential for heterothallic and homothallic sexual reproduction of Volvox. In addition, trioecy (mating system with male, female and bisexual phenotypes) of haploid species was firstly found in the volvocine species Pleodorina starrii. By generating de novo whole genomes of the three phenotypes of P. starrii, molecular genetic bases for evolution of trioecy (drastic reorganization of male and female SDRs of the ancestral heterothallic species) were demonstrated.

Academic Significance and Societal Importance of the Research Achievements

両性の特徴を同時に持つ生物(両性種)がどのように誕生したかを明らかにする目的で、ボルボックスの両性種と近縁種の全ゲノム解析を実施した。その結果、両性種でメスまたはオス側の性染色体の性決定領域の残存が明らかなり、性染色体の両性種における重要性が示唆された。また、3種類の性別(メス、オス、両性型)を持つハプロイド種がボルボックス類のプレオドリナで初めて明らかになった。本種の新規全ゲノム解析から、性染色体のメス・オス特異的遺伝子に大規模な再編成が認められた。3種類の性別が安定的に共存している生物種の存在が示唆され、性別の決定システムと性のダイバーシティーの研究分野に大きく影響することが期待された。

Report

(4 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (41 results)

All 2024 2023 2022 2021 2020 Other

All Int'l Joint Research (4 results) Journal Article (16 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 16 results,  Open Access: 15 results) Presentation (17 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Remarks (4 results)

  • [Int'l Joint Research] Khon Kaen University/Kalasin University(タイ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Khon Kaen Universit/Kalasin University(タイ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Khon Kaen University/Kalasin University(タイ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Donald Danforth Plant Science Center(米国)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Complete mitochondrial and chloroplast DNA sequences of the freshwater green microalga <i>Medakamo hakoo</i>2023

    • Author(s)
      Takusagawa Mari、Misumi Osami、Nozaki Hisayoshi、Kato Shoichi、Maruyama Shinichiro、Tsujimoto-inui Yayoi、Yagisawa Fumi、Ohnuma Mio、Kuroiwa Haruko、Kuroiwa Tsuneyoshi、Matsunaga Sachihiro
    • Journal Title

      Genes & Genetic Systems

      Volume: 98 Issue: 6 Pages: 353-360

    • DOI

      10.1266/ggs.23-00275

    • ISSN
      1341-7568, 1880-5779
    • Year and Date
      2023-12-01
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expanded male sex-determining region conserved during the evolution of homothallism in the green alga Volvox2023

    • Author(s)
      Yamamoto, K., Matsuzaki, R., Mahakham, W., Heman, W., Sekimoto, H., Kawachi, M., Minakuchi, Y., Toyoda, A., Nozaki, H.
    • Journal Title

      iScience

      Volume: 26 Issue: 6 Pages: 106893-106893

    • DOI

      10.1016/j.isci.2023.106893

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evolutionary analysis of MID homologs during the transition from homothallic species to heterothallic species in Volvox sect.Volvox (Chlorophyceae)2023

    • Author(s)
      Yamagishi Shion、Yamamoto Kayoko、Takahashi Kohei、Kawai‐Toyooka Hiroko、Suzuki Shigekatsu、Matsuzaki Ryo、Yamaguchi Haruyo、Kawachi Masanobu、Higashiyama Tetsuya、Nozaki Hisayoshi
    • Journal Title

      Phycological Research

      Volume: 72 Issue: 1 Pages: 46-55

    • DOI

      10.1111/pre.12538

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reorganization of the ancestral sex-determining regions during the evolution of trioecy in Pleodorina starrii2023

    • Author(s)
      Takahashi Kohei、Suzuki Shigekatsu、Kawai-Toyooka Hiroko、Yamamoto Kayoko、Hamaji Takashi、Ootsuki Ryo、Yamaguchi Haruyo、Kawachi Masanobu、Higashiyama Tetsuya、Nozaki Hisayoshi
    • Journal Title

      Communications Biology

      Volume: 6 Issue: 1 Pages: 590-590

    • DOI

      10.1038/s42003-023-04949-1

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo2023

    • Author(s)
      Kato S., Misumi O., Maruyama S., Nozaki H., Tsujimoto-Inui Y., Takusagawa M., Suzuki S., Kuwata K., Noda S., Ito N., Okabe Y., Sakamoto T.,Yagisawa F., Matsunaga T. M., Matsubayashi Y., Yamaguchi H., Kawachi M., Kuroiwa H., Kuroiwa T., Matsunaga S.
    • Journal Title

      Communications Biology

      Volume: 6 Issue: 1 Pages: 89-89

    • DOI

      10.1038/s42003-022-04367-9

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cryopreservation of two species of the multicellular volvocine green algal genus Astrephomene2023

    • Author(s)
      Nozaki Hisayoshi、Mori Fumi、Tanaka Yoko、Matsuzaki Ryo、Yamashita Shota、Yamaguchi Haruyo、Kawachi Masanobu
    • Journal Title

      BMC Microbiology

      Volume: 23 Issue: 1 Pages: 16-16

    • DOI

      10.1186/s12866-023-02767-3

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cryopreservation of vegetative cells and zygotes of the multicellular volvocine green alga Gonium pectorale2022

    • Author(s)
      Nozaki Hisayoshi、Mori Fumi、Tanaka Yoko、Matsuzaki Ryo、Yamaguchi Haruyo、Kawachi Masanobu
    • Journal Title

      BMC Microbiology

      Volume: 22 Issue: 1 Pages: 103-103

    • DOI

      10.1186/s12866-022-02519-9

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Morphology, mating system and taxonomy of Volvox africanus (Volvocaceae, Chlorophyceae) from Thailand2022

    • Author(s)
      Nozaki Hisayoshi、Mahakham Wuttipong、Heman Wirawan、Matsuzaki Ryo、Kawachi Masanobu
    • Journal Title

      Botanical Studies

      Volume: 63 Issue: 1 Pages: 1-1

    • DOI

      10.1186/s40529-022-00332-1

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 湖沼調査から新たなるブレークスルーを目指して2022

    • Author(s)
      野崎久義
    • Journal Title

      藻類 Jpn. J. Phycol.

      Volume: 70 Pages: 1-12

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three sex phenotypes in a haploid algal species give insights into the evolutionary transition to a self-compatible mating system2021

    • Author(s)
      Kohei Takahashi, Hiroko Kawai-Toyooka, Ryo Ootsuki, Takashi Hamaji, Yuki Tsuchikane, Hiroyuki Sekimoto, Tetsuya Higashiyama, Hisayoshi Nozaki
    • Journal Title

      Evolution

      Volume: 75 Issue: 11 Pages: 2984-2993

    • DOI

      10.1111/evo.14306

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid.2021

    • Author(s)
      Matsuzaki R., Suzuki S., Yamaguchi H., Kawachi M., Kanesaki Y., Yoshikawa H., Mori T. and Nozaki H.
    • Journal Title

      BMC Ecology and Evolution

      Volume: 21 Issue: 1 Pages: 11-11

    • DOI

      10.1186/s12862-020-01733-1

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Enigmatic Snow Microorganism, <i>Chionaster nivalis</i>, Is Closely Related to <i>Bartheletia paradoxa</i> (<i>Agaricomycotina</i>, <i>Basidiomycota</i>)2021

    • Author(s)
      Matsuzaki Ryo、Takashima Yusuke、Suzuki Iwane、Kawachi Masanobu、Nozaki Hisayoshi、Nohara Seiichi、Degawa Yousuke
    • Journal Title

      Microbes and Environments

      Volume: 36 Issue: 2 Pages: n/a

    • DOI

      10.1264/jsme2.ME21011

    • NAID

      130008052845

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation2021

    • Author(s)
      Yamashita Shota、Yamamoto Kayoko、Matsuzaki Ryo、Suzuki Shigekatsu、Yamaguchi Haruyo、Hirooka Shunsuke、Minakuchi Yohei、Miyagishima Shin-ya、Kawachi Masanobu、Toyoda Atsushi、Nozaki Hisayoshi
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 22231-22231

    • DOI

      10.1038/s41598-021-01521-x

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The four-celled Volvocales green alga Tetrabaena socialis exhibits weak photobehavior and high-photoprotection ability2021

    • Author(s)
      Tanno Asuka、Tokutsu Ryutaro、Arakaki Yoko、Ueki Noriko、Minagawa Jun、Yoshimura Kenjiro、Hisabori Toru、Nozaki Hisayoshi、Wakabayashi Ken-ichi
    • Journal Title

      PLOS ONE

      Volume: 16 Issue: 10 Pages: e0259138-e0259138

    • DOI

      10.1371/journal.pone.0259138

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Three genomes in the algal genus Volvox reveal the fate of a haploid sex-determining region after a transition to homothallism2021

    • Author(s)
      Kayoko Yamamoto, Takashi Hamaji, Hiroko Kawai-Toyooka, Ryo Matsuzaki, Fumio Takahashi, Yoshiki Nishimura, Masanobu Kawachi, Hideki Noguchi, Yohei Minakuchi, James G. Umen, Atsushi Toyoda, Hisayoshi Nozaki
    • Journal Title

      The Proceedings of the National Academy of Sciences

      Volume: 118 Issue: 21

    • DOI

      10.1073/pnas.2100712118

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A new preferentially outcrossing monoicous species of Volvox sect. Volvox (Chlorophyta) from Thailand2020

    • Author(s)
      Nozaki Hisayoshi、Mahakham Wuttipong、Heman Wirawan、Matsuzaki Ryo、Kawachi Masanobu
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 7 Pages: e0235622-e0235622

    • DOI

      10.1371/journal.pone.0235622

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 細胞質連絡の太いVolvox節2種~琵琶湖よりボルボックス愛をこめて~2024

    • Author(s)
      野崎久義、松﨑令、霜鳥孝一、山口晴代、植木紀子、河地正伸
    • Organizer
      日本藻類学会第48回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Freshwater Algae Bless Us with Exciting Insights into Biology2024

    • Author(s)
      Hisayoshi Nozaki
    • Organizer
      The 9th Asian Pacific Phycological Forum (APPF2024)
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 多細胞性ボルボックス系列全属の凍結保存条件の確立2023

    • Author(s)
      森史,田中陽子,松﨑令,野崎久義,山口晴代,河地正伸
    • Organizer
      日本微生物資源学会第29回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 緑藻 Pleodorina starrii における3性共存進化に伴うゲノム中性決定領域の再編成2023

    • Author(s)
      髙橋昂平, 鈴木重勝, 豊岡博子, 山本荷葉子, 浜地貴志, 大槻涼, 山口晴代, 河地正伸, 東山哲也, 野崎久義
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 松﨑令, 野崎久義, 河地正伸2023

    • Author(s)
      氷雪性オクロモナス (黄金色藻綱) の推定未記載種
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 緑藻ボルボックスの凍結保存株の確立2023

    • Author(s)
      野崎久義、森史、田中陽子、松﨑令、山口晴代、河地正伸
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 4タイプの性表現型をもつ ”ボルボックス・アフリカヌス” の幻の培養株を求め2021

    • Author(s)
      野崎久義・松﨑令・河地正伸
    • Organizer
      日本微生物資源学会第27回大会、オンライン
    • Related Report
      2021 Annual Research Report
  • [Presentation] 湖沼調査から新たなるブレークスルーを目指して2021

    • Author(s)
      野崎久義
    • Organizer
      茨城大学理学部理工学研究科セミナー、オンライン
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] アオミドロ類(接合藻類ストレプト植物門)の接合形態比較及び分子系統解析2021

    • Author(s)
      高野智之・野崎久義・坂山英俊
    • Organizer
      日本植物学会第85回大会、オンライン
    • Related Report
      2021 Annual Research Report
  • [Presentation] アストレフォメネの新規ゲノム解読とボルボックス系列緑藻における非生殖細胞分化の平行進化の解析2021

    • Author(s)
      山下翔大・山本荷葉子・松﨑令・鈴木重勝・山口晴代・廣岡俊亮・水口洋平・宮城島進也・河地正伸・豊田敦・野崎久義
    • Organizer
      日本植物学会第85回大会、オンライン
    • Related Report
      2021 Annual Research Report
  • [Presentation] 群体性ボルボックス目(緑藻類)の恒久的保存を目指して2021

    • Author(s)
      野崎久義・森史・松﨑令・山口晴代・河地正伸
    • Organizer
      日本植物学会第85回大会、オンライン
    • Related Report
      2021 Annual Research Report
  • [Presentation] Three sex phenotypes in a haploid algal species give insights into the evolutionary transition to a self-compatible mating system2021

    • Author(s)
      Takahashi, K., Kawai-Toyooka, H., Ootsuki, R., Hamaji, T., Tsuchikane, Y., Sekimoto, H., Higashiyama, T. & Nozaki1, H.
    • Organizer
      75th Annual Meeting of the Phycological Society of America, Online
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Re-discovery of the third mating system of “Volvox africanus“ from cultures originating from the hometown of dinosaurs in Thailand2021

    • Author(s)
      Nozaki H., Wuttipong, M., Heman, W., Matsuzaki, R. & Kawachi, M.
    • Organizer
      75th Annual Meeting of the Phycological Society of America, Online
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 恐竜の故郷から第3番目の性表現のボルボックス発見2021

    • Author(s)
      野崎久義・Mahakham Wuttipong ・Wirawan Heman ・松﨑令・河地正伸
    • Organizer
      日本植物分類学会第20 回大会、オンライン
    • Related Report
      2020 Annual Research Report
  • [Presentation] 湖沼調査から新たなるブレークスルーを目指して2021

    • Author(s)
      野崎久義
    • Organizer
      日本藻類学会第45回大会、オンライン東京・2021
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 3つの性表現型を持つ緑藻ボルボックス系列 Pleodorina starrii の性決定機構の解析2020

    • Author(s)
      高橋昂平・豊岡博子・大槻涼・浜地貴志・東山哲也・野崎久義
    • Organizer
      日本植物学会第84回大会、オンライン
    • Related Report
      2020 Annual Research Report
  • [Presentation] タイ国産ボルボックス節の他殖性を示す雌雄同体1新種2020

    • Author(s)
      野崎久義・Mahakham Wuttipong ・Wirawan Heman ・松﨑令・河地正伸
    • Organizer
      日本植物学会第84回大会、オンライン
    • Related Report
      2020 Annual Research Report
  • [Remarks] オスの性染色体だけでバイセクシュアル種へ進化する :緑藻ボルボックスの非モデル種の全ゲノム解析で解明

    • URL

      https://www.nies.go.jp/whatsnew/2023/20230622/20230622-2.html

    • Related Report
      2022 Annual Research Report
  • [Remarks] 3タイプの性別を藻類・菌類の同一種内で初めて発見

    • URL

      https://www.s.u-tokyo.ac.jp/ja/press/2021/7451/

    • Related Report
      2021 Annual Research Report
  • [Remarks] Species of algae with three sexes

    • URL

      https://www.u-tokyo.ac.jp/focus/en/press/z0508_00186.html

    • Related Report
      2021 Annual Research Report
  • [Remarks] 雌雄同体の新種誕生におけるメスとオスの性染色体の運命

    • URL

      https://www.s.u-tokyo.ac.jp/ja/press/2021/7373/

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2020-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi