Analyses of mechanisms of the trade-off in altitudinal adaptation in relation to phenotypic plasticity

• Project/Area Number: 20H03317
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 45040:Ecology and environment-related
• Research Institution: Tohoku University
• Principal Investigator: Hikosaka Kouki 東北大学, 生命科学研究科, 教授 (10272006)
• Co-Investigator(Kenkyū-buntansha): 花田 耕介 九州工業大学, 大学院情報工学研究院, 教授 (50462718)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2022: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2021: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2020: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: 標高適応 / 低温耐性 / F2雑種解析 / ゲノムワイド関連解析 / 局所適応 / ゲノム比較 / エコタイプ / 自然変異 / ストレス応答 / 遺伝子解析 / 局所進化 / 遺伝子変異 / 温度適応 / 標高傾度 / 環境応答 / 標高 / 種内変異 / 選択 / ゲノム解析

Outline of Research at the Start

多数のF2個体をストレス環境あるいは現地の環境で育成し、特徴的な表現型（高い/低いストレス耐性、高い/低い生存率・高い/低い成長速度など）をもつ個体の全ゲノム解析を行う。QTL解析により、それぞれの表現型に貢献する遺伝子を特定する。初年度は人工気象機における育成と現地への移植実験を開始し、二年度・三年度に表現型データの解析とゲノム解析を行い、遺伝子を特定する。

Outline of Final Research Achievements

We studied traits and genome of high and low altitudinal ecotypes of Arabidopsis halleri to find traits and genes related to altitudinal adaptations and trade-offs for their background. We produced many F2 hybrids of high and low latitudinal ecotypes and analyzed their tolerance to freezing temperature and photoinhibition at low temperatures. We found that high altitudinal ecotype is more tolerant to both freezing temperature and photoinhibition at low temperatures. We performed the genome-wide association analysis for the traits and detected several genes that are correlated with freezing or photoinhibition tolerance. We also found a trade-off between tolerance to photoinactivation of photosystem II and rate of recovery in photoinhibited photosystem II.

Academic Significance and Societal Importance of the Research Achievements

標高適応に関連した低温耐性がエコタイプ間で違うことを明らかにしたとともに、関連する遺伝子の特定に成功した。これらの知見は、温暖化を含む生物の温度応答の理解を深めるとともに、低温耐性作物の作出に貢献する可能性がある。

Research Products

• [Journal Article] Heterotic growth of hybrids of <i>Arabidopsis thaliana</i> is enhanced by elevated atmospheric CO₂ (2024)
  • Author(s): Mishio Masako、Sudo Emi、Ozaki Hiroshi、Oguchi Riichi、Fujimoto Ryo、Fujii Nobuharu、Hikosaka Kouki
  • Journal Title: American Journal of Botany Volume: 111 Issue: 4
  • DOI: 10.1002/ajb2.16317
  • Peer Reviewed / Open Access
• [Journal Article] Competitive asymmetry in a forest composed of a shade-tolerant species depends on gap formation (2023)
  • Author(s): Yamakawa Masahiro、Onoda Yusuke、Kurokawa Hiroko、Oguro Michio、Nakashizuka Tohru、Hikosaka Kouki
  • Journal Title: Forest Ecology and Management Volume: 549 Pages: 121442-121442
  • DOI: 10.1016/j.foreco.2023.121442
  • Peer Reviewed / Open Access
• [Journal Article] Does selection occur at the intermediate zone of two insufficiently isolated populations? A whole-genome analysis along an altitudinal gradient (2022)
  • Author(s): Yoshida Naofumi、Morinaga Shin-Ichi、Wakamiya Takeshi、Ishii Yuu、Kubota Shosei、Hikosaka Kouki
  • Journal Title: Journal of Plant Research Volume: 136 Issue: 2 Pages: 183-199
  • DOI: 10.1007/s10265-022-01429-1
  • Peer Reviewed
• [Journal Article] Predicting diversity changes in subalpine moorland ecosystems based on geometry of species distributions and realistic area loss (2022)
  • Author(s): Makishima Daichi、Ishii Naohiro、Sutoh Rui、Goto Akihito、Kawai Yutaka、Taniguchi Hayami、Uchida Kei、Shimazaki Masaya、Nakashizuka Tohru、Suyama Yoshihisa、Hikosaka Kouki、Sasaki Takehiro
  • Journal Title: Journal of Vegetation Science Volume: 33 Issue: 5
  • DOI: 10.1111/jvs.13150
  • Peer Reviewed
• [Journal Article] Plant and microbial community composition jointly determine moorland multifunctionality (2022)
  • Author(s): Sasaki Takehiro、Ishii Naohiro I.、Makishima Daichi、Sutou Rui、Goto Akihito、Kawai Yutaka、Taniguchi Hayami、Okano Kunihiro、Matsuo Ayumi、Lochner Alfred、Cesarz Simone、Suyama Yoshihisa、Hikosaka Kouki、Eisenhauer Nico
  • Journal Title: Journal of Ecology Volume: 110 Issue: 10 Pages: 2507-2521
  • DOI: 10.1111/1365-2745.13969
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Enhanced growth rate under elevated CO2 conditions was observed for transgenic lines of genes identified by intraspecific variation analyses in Arabidopsis thaliana (2022)
  • Author(s): Oguchi Riichi、Hanada Kousuke、Shimizu Minami、Mishio Masako、Ozaki Hiroshi、Hikosaka Kouki
  • Journal Title: Plant Molecular Biology Volume: 110 Issue: 4-5 Pages: 333-345
  • DOI: 10.1007/s11103-022-01265-w
  • Peer Reviewed
• [Journal Article] The latitudinal and altitudinal variations in the biochemical mechanisms of temperature dependence of photosynthesis within Fallopia japonica (2021)
  • Author(s): Machino Satoshi、Nagano Soichiro、Hikosaka Kouki
  • Journal Title: Environmental and Experimental Botany Volume: 181 Pages: 104248-104248
  • DOI: 10.1016/j.envexpbot.2020.104248
  • Peer Reviewed
• [Journal Article] Intraspecific variations in leaf traits, productivity and resource use efficiencies in the dominant species of subalpine evergreen coniferous and deciduous broad‐leaved forests along the altitudinal gradient (2021)
  • Author(s): Hikosaka Kouki、Kurokawa Hiroko、Arai Takahisa、Takayanagi Sakino、Tanaka Hiroshi O.、Nagano Soichiro、Nakashizuka Tohru
  • Journal Title: Journal of Ecology Volume: 109 Issue: 4 Pages: 1804-1818
  • DOI: 10.1111/1365-2745.13603
  • Peer Reviewed
• [Journal Article] Variations in leaf economics spectrum traits for an evergreen coniferous species: Tree size dominates over environment factors (2020)
  • Author(s): Liu Zhili、Hikosaka Kouki、Li Fengri、Jin Guangze
  • Journal Title: Functional Ecology Volume: 34 Issue: 2 Pages: 458-467
  • DOI: 10.1111/1365-2435.13498
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Temperature-related cline in the root mass fraction in East Asian wild radish along the Japanese archipelago (2020)
  • Author(s): Ishizuka Wataru、Hikosaka Kouki、Ito Motomi、Morinaga Shin-Ichi
  • Journal Title: Breeding Science Volume: 70 Issue: 3 Pages: 321-330
  • DOI: 10.1270/jsbbs.18201
  • NAID: 130007866575
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access
• [Journal Article] Plant size, environmental factors and functional traits jointly shape the stem radius growth rate in an evergreen coniferous species across ontogenetic stages (2020)
  • Author(s): Liu Zhili、Hikosaka Kouki、Li Fengri、Zhu Liangjun、Jin Guangze
  • Journal Title: Journal of Plant Ecology Volume: 14 Issue: 2 Pages: 257-269
  • DOI: 10.1093/jpe/rtaa093
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dividing the pie: A quantitative review on plant density responses (2020)
  • Author(s): Postma Johannes A.、Hecht Vera L.、Hikosaka Kouki、Nord Eric A.、Pons Thijs L.、Poorter Hendrik
  • Journal Title: Plant, Cell & Environment Volume: 44 Issue: 4 Pages: 1072-1094
  • DOI: 10.1111/pce.13968
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Remote sensing of photosynthesis and stress responses in leaves and vegetations (2023)
  • Author(s): Kouki Hikosaka
  • Organizer: International Biology Conference (IBOC) 2023
  • Int'l Joint Research / Invited
• [Presentation] ハクサンハタザオの低温耐性の標高間変異：F2集団の形質・ゲノムから探る遺伝的背景 (2023)
  • Author(s): 吉田直史, 森長真一, 石井悠, 白井一正, 久保田渉誠, 花田耕介, 彦坂幸毅
  • Organizer: 日本生態学会第70回全国大会
• [Presentation] ハクサンハタザオの低温ストレス耐性の標高間変異:エコタイプ間比較および F2 集団の観察から見えてくる遺伝的背景 (2022)
  • Author(s): 吉田直史,森長真一,彦坂幸毅
  • Organizer: 東北植物学会 第 12 回大会 (宮城大会)
• [Presentation] ハクサンハタザオの高標高、低標高エコタイプにおける光合成の温度応答特性 (2022)
  • Author(s): 桑田直樹，吉田直史，彦坂幸毅
  • Organizer: 東北植物学会 第 12 回大会 (宮城大会)
• [Presentation] リモートセンシングパラメータと葉光合成速度の関係 (2021)
  • Author(s): 彦坂幸毅, 辻本克斗
  • Organizer: 第68回日本生態学会大会
• [Presentation] Remote sensing of photosynthesis (2020)
  • Author(s): Kouki Hikosaka
  • Organizer: Astrobiology center symposium 2020
  • Int'l Joint Research / Invited
• [Presentation] Analysis of the genetic background of invasive species along latitude by using SNP information (2020)
  • Author(s): 瀋 毅恒，尾崎 洋史，吉田 直史，森長 真一，彦坂 幸毅
  • Organizer: 日本植物学会第84回大会
• [Presentation] 光阻害が起こった葉における光合成・クロロフィル蛍光・ 光化学反射指数(PRI) (2020)
  • Author(s): 彦坂幸毅
  • Organizer: 日本植物学会第84回大会
• [Remarks] 東北大学生命科学研究科機能生態分野
  • URL: http://www.biology.tohoku.ac.jp/lab-www/hikosaka_lab/
• [Remarks] 彦坂幸毅のページ
  • URL: http://www.biology.tohoku.ac.jp/lab-www/hikosaka_lab/hikosaka/index.html