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Associations between plants and bacteria (Sphingomonas spp.) dominated on the anthosphere and their geographic variation

Research Project

Project/Area Number 20H03324
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionResearch Institute for Humanity and Nature (2023)
Kyoto University (2020-2022)

Principal Investigator

Sakai Shoko  総合地球環境学研究所, 研究基盤国際センター, 客員教授 (30361306)

Co-Investigator(Kenkyū-buntansha) 乾 陽子  大阪教育大学, 教育学部, 准教授 (10343261)
辻 かおる  京都大学, 理学研究科, 研究員 (40645280)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2023: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2022: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2021: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywords送粉 / 細菌 / アンプリコンシーケンシング / 微生物叢 / 花圏微生物叢 / スフィンゴモナス属 / 細菌叢 / 花圏微生物 / 雌雄異株 / 種子微生物 / 植物繁殖生態 / 共生関係 / 相利共生
Outline of Research at the Start

植物表面には地下のみならず地上部であっても、膨大な数の微生物が存在する。葉表面には植物の種や遺伝子型に応じた共生微生物が棲息し、耐病性や成長に寄与している。しかし、繁殖器官である花の微生物叢についての知見は、葉に比べると非常に少ない。ところが申請者らは最近、トウダイグサ科アカメガシワ雌花上で、スフィンゴモナス属細菌(以下、スフィンゴモナス)が優占することを見出した。スフィンゴモナスは、葉では、植物の耐病性を高めていることが報告されている。そこで本研究では、アカメガシワ雌花のスフィンゴモナスが、寄主植物の病原生物からの防衛に貢献し、相利共生関係にあることを示す。

Outline of Final Research Achievements

This research focused on Sphingomonas bacteria dominating on the stigmas of female Mallotus japonicus (Euphorbiaceae) and explored their interaction with the host plants. We found that the composition of Sphingomonas on the stigmas changed across flowering seasons at the same sampling site. While the bacterial composition also differed among the sampling sites, there was no clear geographical pattern as anticipated. Sphinogomonas bacteria are also found as seed endophytes of the host plant. Our inoculation experiment suggested that Sphingomonas bacteria on the stigmas affect the endophyte compositions and diversity of the resultant seeds. In summary, this study showed that microbes on flower can impact the next generation through the transmission of seed endophytes, whereas their association with the host plant may be rather stochastic.

Academic Significance and Societal Importance of the Research Achievements

これまで植物と地下微生物の相互作用については、進化・生態学重要性と食物生産上の観点から盛んに調べられて来た。一方、植物繁殖器官上の微生物群集やそれらの繁殖成功への影響は、ほとんど顧みられて来なかった。花は一般に寿命が短く微生物叢は重要でないと考えられてきたこと、種子にはほとんど微生物がいないと考えられてきたことが、その背景にあると考えられる。本研究では、野生植物の花・種子上の微生物と宿主植物の関係に焦点をあて、送粉者によって運ばれる細菌の潜在的インパクト、雄雌の差、年変動や地理的変異を明らかにした。繁殖器官上の微生物は、植物の繁殖成功に大きな影響を与える重要な要素であることが示唆された。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (16 results)

All 2024 2023 2022 2021 2020

All Journal Article (5 results) (of which Open Access: 3 results,  Peer Reviewed: 2 results) Presentation (11 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results)

  • [Journal Article] Partitioning the contribution of bees with different traits and hoverflies to flower-visitor interaction networks2024

    • Author(s)
      Martinez-Nuez Carlos, Sakai Shoko
    • Journal Title

      Ecological Indicators

      Volume: 163 Pages: 112041-112041

    • DOI

      10.1016/j.ecolind.2024.112041

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Idea paper: Extended benefits of pollinator‐mediated microbial dispersal among flowers2022

    • Author(s)
      Takeda Kazuya、Sakai Shoko
    • Journal Title

      Ecological Research

      Volume: 37 Issue: 4 Pages: 481-484

    • DOI

      10.1111/1440-1703.12326

    • Related Report
      2022 Annual Research Report
    • Open Access
  • [Journal Article] Are microbes growing on flowers evil? Effects of old flower microbes on fruit set in a wild ginger with one-day flowers, Alpinia japonica (Zingiberaceae)2022

    • Author(s)
      Jimenez Elvira Nuria、Ushio Masayuki、Sakai Shoko
    • Journal Title

      Metabarcoding and Metagenomics

      Volume: 6

    • DOI

      10.3897/mbmg.6.84331

    • Related Report
      2022 Annual Research Report
    • Open Access
  • [Journal Article] The effects of the floral infection by a bacterial pathogen in a dioecious plant, Mallotus japonicus (Euphorbiaceae)2022

    • Author(s)
      Marre M, Ushio M, Sakai S
    • Journal Title

      Population Ecology

      Volume: - Issue: 4 Pages: 231-239

    • DOI

      10.1002/1438-390x.12110

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contrasting microbial communities on male and female flowers of a dioecious plant, Mallotus japonicus (Euphorbiaceae).2021

    • Author(s)
      Marre M, Ushio M, Sakai S
    • Journal Title

      Environmental DNA

      Volume: - Issue: 3 Pages: 1-9

    • DOI

      10.1002/edn3.271

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Factors Affecting Variation in Seed Bacterial Communities of Wild Tree, Mallotus japonicus2023

    • Author(s)
      Marin Komojiri, Masayuki Ushio, Kazuya Takeda, Shoko Sakai
    • Organizer
      The eDNA Society International Meeting 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of floral bacteria by their context-dependency and host specificity2023

    • Author(s)
      Kazuya Takeda, Shoko Sakai
    • Organizer
      The eDNA Society International Meeting 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ツルニンジン花上細菌叢に花弁表面ワックスが与える影響2023

    • Author(s)
      武田 和也・酒井 章子
    • Organizer
      日本生態学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 野生樹木アカメガシワにおける種子微生物叢の変動要因2023

    • Author(s)
      小茂尻 真凜・潮 雅之・武田 和也・酒井 章子
    • Organizer
      日本生態学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 見過ごされてきた訪花者:花をめぐる細菌とその役割2022

    • Author(s)
      酒井 章子
    • Organizer
      The 2nd Symposium of Plant Microbiota Research Network
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] アカメガシワの種子内生菌2022

    • Author(s)
      小茂尻真凜, 酒井章子
    • Organizer
      日本生態学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 花に見るオスの都合・メスの事情2021

    • Author(s)
      酒井章子
    • Organizer
      植物分類学会講演会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Why are flowers ephemeral? Effects of old flower microbes on fruit set in a wild ginger with one-day flowers, Alpinia japonica (Zingiberaceae)2021

    • Author(s)
      Shoko Sakai
    • Organizer
      Plant Microbiota Research Network (PMRN)(オンライン)
    • Related Report
      2021 Annual Research Report
  • [Presentation] Contrasting microbiomes of male and female flowers of Mallotus japonics2020

    • Author(s)
      Marre Maxime, Masayuki Ushio, Shoko Sakai
    • Organizer
      日本生態学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] アカメガシワ斑点細菌病の花器感染2020

    • Author(s)
      酒井章子・Marre Maxime・潮雅之
    • Organizer
      植物病理学会・関西支部会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Microbes on flowers and pollination2020

    • Author(s)
      Shoko SAKAI
    • Organizer
      環境DNA学会・個体群生態学会合同大会
    • Related Report
      2020 Annual Research Report
    • Invited

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Published: 2020-04-28   Modified: 2025-01-30  

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