| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2022: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2021: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2020: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Outline of Final Research Achievements |
This research aimed to understand the mechanisms of cell assembly development and formation by imaging using hippocampal place cells as a model. The study extended a calcium imaging of deep brain neural circuits in behaving animals that the principal investigator of this study had established in the past to imaging of developing mice, which is technically more challenging. We have developed two-photon calcium imaging of the mouse hippocampus with fluorescent-labeled specific pyramidal cell populations during development. We have also succeeded in establishing important fundamental technologies such as gene transfer in the neonatal rat hippocampus, construction of a virtual reality system for postnatal rats and fluorescent membrane voltage imaging using an sCMOS camera and a custom microscope.
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