| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2022: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2021: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
Mouse fibroblasts secreting each morphogen among five of those were generated and transplanted into the dorsal column of cervical spinal cord in mice to create a gradient of morphogens. Subsequently, a dorsal column lesion of the cervical spinal cord was created, followed by the graft of neural progenitor cells derived from mouse embryonic spinal cord were transplanted into the lesion site. The evaluation of patterning of transplanted neurons demonstrated no apparent changes in positions of grafted neurons, suggesting that the grafted neurons into lesion sites are insensitive to a gradient of morphogens or require a gradient of multiple morphogens to change their locations like developing spinal cord. Although the further experiment is necessary to examine the effect of a gradient of multiple morphogens, the newly developed methodology to create a gradient of soluble factors in spinal cord will advance the technology of tissue engineering in neuroscience.
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