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Novel method of skin metatranscriptome analysis for predicting the onset of skin disease

Research Project

Project/Area Number 20H03701
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 53050:Dermatology-related
Research InstitutionOsaka University

Principal Investigator

MATSUOKA YUMI  大阪大学, 免疫学フロンティア研究センター, 教授 (10402067)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2022: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2021: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2020: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywordsアトピー性皮膚炎 / トランスクリプトーム / 発症前 / 皮膚細菌叢 / 予防 / 細菌叢 / 発症予測 / マイクロバイオーム / アレルギーマーチ / バイオマーカー / 常在微生物叢 / バイオマーカー探索 / 微生物叢 / メタトランスクリプトーム
Outline of Research at the Start

アトピー性皮膚炎およびその動物モデルのデータから、数理モデルなどの手法を用いて、発症前のバイオマーカー探索を行う。このバイオマーカーはアレルギー疾患予防のための早期介入指標となりうると考えられる。また、そのバイオマーカーが疾患においてどのような役割を果たしているのかを培養系および動物モデルなどを用いて解析することで、免疫系と微生物叢の皮膚における相互関係を探索する。

Outline of Final Research Achievements

We performed a metatranscriptomic analysis using amplified RNA from non-invasive swab sampling of human atopic dermatitis, complemented by gene expression data from pre-disease skin in a naturally occurring mouse model of atopic dermatitis obtained from existing databases. This confirmed the alignment between human and mouse transcriptomic data. Building on previous research that linked the skin microbiota of neonates at three days old to the development of atopic dermatitis by age one, we focused on analyzing the neonatal transcriptome. We found many genes significantly differentially expressed between the groups with and without atopic dermatitis onset, which mirrored the expression patterns in disease and healthy groups during the mouse pre-disease stage. Furthermore, we successfully identified a novel target gene X (unpublished) as a potential intervention point in the pre-disease stage for effective disease prevention.

Academic Significance and Societal Importance of the Research Achievements

我々の確立した系では、皮膚生検などの侵襲的な処置を必要としないため、同一部位から経時的に繰り返し、サンプリングを行うことができる。こういった背景を鑑みると、我々が確立した宿主・菌叢メタトランスクリプトームはまさに発症前の皮膚疾患を解析するのに最適な解析法と考えている。本解析法を用い、ADの発症予防のためのバイオマーカーを探索できたことそのものも学術的に非常に意義深いと考えているが、今後疾患での予防効果などを具体的に示すことができれば、治療法の創出にもつながると考えている。

Report

(4 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (9 results)

All 2024 2023 2022 2021 2020 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 8 results,  Open Access: 5 results)

  • [Int'l Joint Research] University of Michigan(米国)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Neonatal skin dysbiosis to infantile atopic dermatitis: Mitigating effects of skin care2024

    • Author(s)
      Aoyama Reika、Nakagawa Seitaro、Ichikawa Yoko、Inohara Naohiro、Yamazaki Yuriko、Ito Tomoka、Sugihira Takashi、Kono Michihiro、Akiyama Masashi、Takahashi Hiroki、Takaya Akiko、Ichikawa Fumitaka、Nakano Taiji、Tanaka Seiko、Koyano Yutaka、Fujimoto Manabu、Nunez Gabriel、Shimojo Naoki、Nakamura Yuumi
    • Journal Title

      Allergy

      Volume: 0 Issue: 6 Pages: 1-5

    • DOI

      10.1111/all.16095

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The role of Staphylococcus aureus quorum sensing in cutaneous and systemic infections2024

    • Author(s)
      Yamazaki Yuriko、Ito Tomoka、Tamai Masakazu、Nakagawa Seitaro、Nakamura Yuumi
    • Journal Title

      Inflammation and Regeneration

      Volume: 44 Issue: 1

    • DOI

      10.1186/s41232-024-00323-8

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pathogenic role of the staphylococcal accessory gene regulator quorum sensing system in atopic dermatitis2023

    • Author(s)
      Tamai Masakazu、Yamazaki Yuriko、Ito Tomoka、Nakagawa Seitaro、Nakamura Yuumi
    • Journal Title

      Frontiers in Cellular and Infection Microbiology

      Volume: 13

    • DOI

      10.3389/fcimb.2023.1178650

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Epidermal clearance of <i>Candida albicans</i> is mediated by IL-17 but independent of fungal innate immune receptors2022

    • Author(s)
      Iwasawa Mari T、Miyachi Hideaki、Wakabayashi Seiichiro、Sugihira Takashi、Aoyama Reika、Nakagawa Seitaro、Katayama Yuki、Yoneyama Mitsutoshi、Hara Hiromitsu、Iwakura Yoichiro、Matsumoto Masanori、Inohara Naohiro、Koguchi-Yoshioka Hanako、Fujimoto Manabu、N??ez Gabriel、Matsue Hiroyuki、Nakamura Yuumi、Saijo Shinobu
    • Journal Title

      International Immunology

      Volume: 34 Issue: 8 Pages: 409-420

    • DOI

      10.1093/intimm/dxac019

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Effects of Dietary Supplementation of Lactococcus lactis Strain Plasma on Skin Microbiome and Skin Conditions in Healthy Subjects?A Randomized, Double-Blind, Placebo-Controlled Trial2021

    • Author(s)
      Tsuji Ryohei、Yazawa Kamiyu、Kokubo Takeshi、Nakamura Yuumi、Kanauchi Osamu
    • Journal Title

      Microorganisms

      Volume: 9 Issue: 3 Pages: 563-563

    • DOI

      10.3390/microorganisms9030563

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Keratinocyte IL-36 Receptor/MyD88 Signaling Mediates <i>Malassezia</i>-Induced IL-17?Dependent Skin Inflammation2021

    • Author(s)
      Miyachi Hideaki、Wakabayashi Seiichiro、Sugihira Takashi、Aoyama Reika、Saijo Shinobu、Koguchi-Yoshioka Hanako、Fujimoto Manabu、N??ez Gabriel、Matsue Hiroyuki、Nakamura Yuumi
    • Journal Title

      The Journal of Infectious Diseases

      Volume: 223 Issue: 10 Pages: 1753-1765

    • DOI

      10.1093/infdis/jiab194

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Interaction between Staphylococcus Agr virulence and neutrophils regulates pathogen expansion in the skin2021

    • Author(s)
      Matsumoto Masanori、Nakagawa Seitaro、Zhang Lingzhi、Nakamura Yuumi、Villaruz Amer E.、Otto Michael、Wolz Christiane、Inohara Naohiro、Nunez Gabriel
    • Journal Title

      Cell Host & Microbe

      Volume: 29 Issue: 6 Pages: 930-940.e4

    • DOI

      10.1016/j.chom.2021.03.007

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Staphylococcus Agr virulence is critical for epidermal colonization and associates with atopic dermatitis development2020

    • Author(s)
      Nakamura Y, Takahashi H, Takaya A, Inoue Y, Katayama Y, Kusuya Y, Shoji T, Takada S, Nakagawa S, Oguma R, Saito N, Ozawa N, Nakano T, Yamaide F, Dissanayake E, Suzuki S, Villaruz A, Varadarajan S, Matsumoto M, Kobayashi T, Kono M, Sato Y, Akiyama M, Otto M, Matsue H, Nunez G, Shimojo N.
    • Journal Title

      Sci Transl Med.

      Volume: 8 Issue: 551 Pages: 4068-4068

    • DOI

      10.1126/scitranslmed.aay4068

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research

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Published: 2020-04-28   Modified: 2025-01-30  

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