Functional analysis of small intestinal epithelial organoid-based transplant graft

• Project/Area Number: 20H03746
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 55010:General surgery and pediatric surgery-related
• Research Institution: Keio University
• Principal Investigator: SUGIMOTO Shinya 慶應義塾大学, 医学部(信濃町), 助教 (20626387)
• Co-Investigator(Kenkyū-buntansha): 袴田 陽二 日本獣医生命科学大学, 獣医学部, 教授 (00218380) ; 小林 英司 慶應義塾大学, 医学部(信濃町), 特任教授 (00245044)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2022: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2021: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2020: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: 短腸症候群 / 小腸移植 / 幹細胞 / 細胞移植 / 組織修復 / 上皮置換 / 潰瘍性大腸炎 / 炎症性腸疾患 / オルガノイド / 体性幹細胞 / 小腸疾患 / 再生機構 / 臓器再生 / 潰瘍性大腸炎関連腫瘍 / 細胞競合 / 吸収機能

Outline of Research at the Start

腸管上皮幹細胞をオルガノイドとして永続的に体外培養可能となり，研究ツールとしてのみならず，再生医療への応用も期待されている．とりわけ，十分な治療法がなく命を落とす患者の多い難治性小腸疾患に対する革新的な治療法の開発が望まれている．本研究では，ラットおよびヒト小腸上皮オルガノイドを用いて，大腸上皮を小腸上皮オルガノイドで置換することで小腸上皮化大腸を創出し，その機能解析を行うことを目的とする．

Outline of Final Research Achievements

Combining the organoid transplantation technique with surgery, we developed a technique to create small intestinalized colon grafts by removing a portion of colonic epithelium from rats and transplanting organoids derived from the small intestine. Functional analysis of intestinal organoids, human small intestinal epithelium transplanted into mouse colon, and small intestinalized colon grafts in rats was performed. We successfully demonstrated the small intestinal function of the epithelium constructed by small intestinal epithelial organoids and confirmed the concept of organoid-based therapy for a small animal model of short bowel syndrome.

Academic Significance and Societal Importance of the Research Achievements

小腸上皮は移植した先の組織の構造を変化させ、小腸としての機能を発揮しうることが明らかになった。大腸の上皮を小腸オルガノイドにより置換した小腸化大腸を移植するという新しい治療戦略は、今回コンセプトが確認された小動物（マウス、ラット）と比較してサイズが大きなヒトにおいても応用可能と考えられる。今後、自己の小腸オルガノイドと大腸組織を用いた自家移植を行うことで、免疫抑制剤を必要としない新規治療法の開発へとつながることが期待される。

Research Products

• [Journal Article] Water Does Not Mix with Lipids: Water-assisted Colonoscopy (2023)
  • Author(s): Sugimoto Shinya、Takabayashi Kaoru、Kanai Takanori
  • Journal Title: Internal Medicine Volume: 62 Issue: 4 Pages: 665-666
  • DOI: 10.2169/internalmedicine.0053-22
  • ISSN: 0918-2918, 1349-7235
  • Year and Date: 2023-02-15
  • Peer Reviewed / Open Access
• [Journal Article] Usefulness of texture and color enhancement imaging in assessing mucosal healing in patients with ulcerative colitis (2023)
  • Author(s): Hayashi Yukie、Takabayashi Kaoru、Kato Motohiko、Tojo Anna、Aoki Yasuhiro、Hagihara Yuya、Yoshida Kosuke、Yoshimatsu Yusuke、Kiyohara Hiroki、Sugimoto Shinya、Nanki Kosaku、Mikami Yohei、Sujino Tomohisa、Mutaguchi Makoto、Kawaguchi Takaaki、Hosoe Naoki、Yahagi Naohisa、Ogata Haruhiko、Kanai Takanori
  • Journal Title: Gastrointestinal Endoscopy Volume: 97 Issue: 4 Pages: 759-766.e1
  • DOI: 10.1016/j.gie.2022.11.019
  • Peer Reviewed / Open Access
• [Journal Article] DEVELOPMENT AND APPLICATION OF WATER-AIDED COLONOSCOPY (2023)
  • Author(s): 水上 健、杉本 真也
  • Journal Title: GASTROENTEROLOGICAL ENDOSCOPY Volume: 65 Issue: 1 Pages: 19-28
  • DOI: 10.11280/gee.65.19
  • ISSN: 0387-1207, 1884-5738
  • Peer Reviewed
• [Journal Article] Efficacy of Calcineurin Inhibitors for Induction of Remission in Intestinal Beh?et’s Disease (2022)
  • Author(s): Kawaguchi Takaaki、Fukata Masayuki、Omori Teppei、Kiyohara Hiroki、Sugimoto Shinya、Nanki Kosaku、Sujino Tomohisa、Mikami Yohei、Kanai Takanori
  • Journal Title: Crohn's & Colitis 360 Volume: 4 Issue: 3 Pages: 1-10
  • DOI: 10.1093/crocol/otac017
  • Peer Reviewed / Open Access
• [Journal Article] Effectiveness and Durability of Ustekinumab Therapy With or Without Immunomodulators for Ulcerative Colitis Patients in Japan (2022)
  • Author(s): Aoki Yasuhiro、Sujino Tomohisa、Kawaguchi Takaaki、Sugimoto Shinya、Shimada Fumie、Yoshimatsu Yusuke、Kiyohara Hiroki、Nanki Kosaku、Mikami Yohei、Takabayashi Kaoru、Hosoe Naoki、Ogata Haruhiko、Iwao Yasushi、Kanai Takanori
  • Journal Title: Crohn's & Colitis 360 Volume: 4 Issue: 2 Pages: 1-10
  • DOI: 10.1093/crocol/otac010
  • Peer Reviewed / Open Access
• [Journal Article] <i>In Vivo</i> Intestinal Research Using Organoid Transplantation (2022)
  • Author(s): Sugimoto Shinya、Kobayashi Eiji、Kanai Takanori、Sato Toshiro
  • Journal Title: The Keio Journal of Medicine Volume: 71 Issue: 4 Pages: 73-81
  • DOI: 10.2302/kjm.2022-0019-IR
  • ISSN: 0022-9717, 1880-1293
  • Peer Reviewed / Open Access
• [Journal Article] CXCL16 inhibits epithelial regeneration and promotes fibrosis during the progression of radiation enteritis (2022)
  • Author(s): Cui Yanmei、Wu Haiyong、Liu Zhihang、Ma Tenghui、Liang Wenfeng、Zeng Qingzhi、Chen Daici、Qin Qiyuan、Huang Binjie、Wang Michael Hu、Huang Xiaoyan、He Yanjiong、Kuang Yingyi、Sugimoto Shinya、Sato Toshiro、Wang Lei
  • Journal Title: The Journal of Pathology Volume: 259 Issue: 2 Pages: 180-193
  • DOI: 10.1002/path.6031
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of Quiescent LGR5+ Stem Cells in the Human Colon (2022)
  • Author(s): Ishikawa K, Sugimoto S, Oda M, Fujii M, Toshimitsu K, Sawada K, Shimokawa M, Saito M, Kawasaki K, Ishii R, Taniguchi K, Sato T.
  • Journal Title: Gastroenterology. Volume: 163 Issue: 5 Pages: 1391-1406
  • DOI: 10.1053/j.gastro.2022.07.081
  • Peer Reviewed / Open Access
• [Journal Article] Texture and color enhancement imaging in combination with indigo carmine dye spraying to highlight the border of flat ulcerative colitis-associated neoplasia (2022)
  • Author(s): Takabayashi Kaoru、Kato Motohiko、Sugimoto Shinya、Yahagi Naohisa、Kanai Takanori
  • Journal Title: Gastrointestinal Endoscopy Volume: 95 Issue: 6 Pages: 1273-1275
  • DOI: 10.1016/j.gie.2022.02.011
  • Peer Reviewed
• [Journal Article] 【内視鏡データリファレンスブック2022】臓器別 大腸 潰瘍性大腸炎関連腫瘍 (2022)
  • Author(s): 岩男 泰, 杉本 真也, 高林 馨, 三上 洋平, 金井 隆典
  • Journal Title: 消化器内視鏡 Volume: 34 Pages: 735-740
• [Journal Article] 潰瘍性大腸炎に伴うdysplasiaに対する内視鏡治療はどのように考えるのか? (2022)
  • Author(s): 高林 馨, 杉本 真也, 吉松 裕介, 清原 裕貴, 三上 洋平, 筋野 智久, 河口 貴昭, 加藤 元彦, 細江 直樹, 岩男 泰, 矢作 直久, 金井 隆典, 緒方 晴彦
  • Journal Title: 消化器・肝臓内科 Volume: 12 Pages: 158-164
• [Journal Article] 直腸扁平上皮化と潰瘍性大腸炎関連腫瘍の領域拡大における上皮置換の寄与 (2022)
  • Author(s): 杉本 真也, 岩男 泰, 金井 隆典
  • Journal Title: 消化器病学サイエンス Volume: 6 Pages: 186-189
• [Journal Article] 潰瘍性大腸炎関連発がんの臨床 (2022)
  • Author(s): 杉本 真也, 金井 隆典
  • Journal Title: 別冊Bio Clinica Volume: 11 Pages: 47-52
• [Journal Article] Epithelium Replacement Contributes to Field Expansion of Squamous Epithelium and Ulcerative Colitis?Associated Neoplasia (2022)
  • Author(s): Sugimoto Shinya、Iwao Yasushi、Shimoda Masayuki、Takabayashi Kaoru、Sato Toshiro、Kanai Takanori、Keio IBD Collaborators
  • Journal Title: Gastroenterology Volume: 162 Issue: 1 Pages: 334-337
  • DOI: 10.1053/j.gastro.2021.09.051
  • Peer Reviewed / Open Access
• [Journal Article] Organoid vs In Vivo Mouse Model: Which is Better Research Tool to Understand the Biologic Mechanisms of Intestinal Epithelium? (2022)
  • Author(s): Sugimoto Shinya、Sato Toshiro
  • Journal Title: Cellular and Molecular Gastroenterology and Hepatology Volume: 13 Issue: 1 Pages: 195-197
  • DOI: 10.1016/j.jcmgh.2021.06.027
  • Peer Reviewed / Open Access
• [Journal Article] Rebuttal to: In Vivo Studies Should Take Priority When Defining Mechanisms of Intestinal Crypt Morphogenesis (2022)
  • Author(s): Sugimoto Shinya、Sato Toshiro
  • Journal Title: Cellular and Molecular Gastroenterology and Hepatology Volume: 13 Issue: 1 Pages: 5-5
  • DOI: 10.1016/j.jcmgh.2021.09.010
  • Peer Reviewed / Open Access
• [Journal Article] Organoid Medicine for Inflammatory Bowel Disease (2022)
  • Author(s): Wakisaka Yusuke、Sugimoto Shinya、Sato Toshiro
  • Journal Title: Stem Cells Volume: 40 Issue: 2 Pages: 123-132
  • DOI: 10.1093/stmcls/sxab020
  • Peer Reviewed / Open Access
• [Journal Article] 腸管上皮オルガノイド (2022)
  • Author(s): 杉本 真也
  • Journal Title: 消化器病学サイエンス Volume: 6 Pages: 49-49
• [Journal Article] オルガノイドを用いた臓器転用アプローチによる短腸症候群治療 (2022)
  • Author(s): 杉本 真也
  • Journal Title: 再生医療 Volume: 21 Pages: 38-41
• [Journal Article] 原著論文 ヒト腸管オルガノイドを用いた市販アルコール系製品のヒトノロウイルスの不活性化効果の評価 (2021)
  • Author(s): 戸高 玲子、藤本 陽、芳賀 慧、股野 麻未、杉本 真也、佐藤 俊朗、片山 和彦
  • Journal Title: 感染制御と予防衛生 Volume: 5 Issue: 2 Pages: 46-54
  • DOI: 10.34449/J0108.05.02_0046-0054
  • ISSN: 2433-4030
  • Year and Date: 2021-12-15
  • Peer Reviewed
• [Journal Article] Nasal delivery of single-domain antibody improves symptoms of SARS-CoV-2 infection in an animal model (2021)
  • Author(s): Haga Kei、Takai-Todaka Reiko、Matsumura Yuta、Song Chihong、Takano Tomomi、Tojo Takuto、Nagami Atsushi、Ishida Yuki、Masaki Hidekazu、Tsuchiya Masayuki、Ebisudani Toshiki、Sugimoto Shinya、Sato Toshiro、Yasuda Hiroyuki、Fukunaga Koichi、Sawada Akihito、Nemoto Naoto、Murata Kazuyoshi、Morimoto Takuya、Katayama Kazuhiko
  • Journal Title: PLOS Pathogens Volume: 17 Issue: 10 Pages: 1009542-1009542
  • DOI: 10.1371/journal.ppat.1009542
  • Peer Reviewed / Open Access
• [Journal Article] Demarcated redness associated with increased vascular density/size: a useful marker of flat-type dysplasia in patients with ulcerative colitis (2021)
  • Author(s): Ikebata Akiyoshi、Shimoda Masayuki、Okabayashi Koji、Uraoka Toshio、Maehata Tadateru、Sugimoto Shinya、Mutaguchi Makoto、Naganuma Makoto、Kameyama Kaori、Yahagi Naohisa、Kanai Takanori、Kitagawa Yuko、Kanai Yae、Iwao Yasushi
  • Journal Title: Endoscopy International Open Volume: 09 Issue: 04 Pages: E552-E561
  • DOI: 10.1055/a-1352-2709
  • Peer Reviewed / Open Access
• [Journal Article] Water-assisted colonoscopy: an international modified Delphi review on definitions and practice recommendations (2021)
  • Author(s): Cadoni Sergio、Ishaq Sauid、Hassan Cesare、Falt Pemysl、Fuccio Lorenzo、Siau Keith、Leung Joseph W.、Anderson John、Binmoeller Kenneth F.、Radaelli Franco、Rutter Matt D.、Sugimoto Shinya、Muhammad Humayun、Bhandari Pradeep、Draganov Peter V. et al.
  • Journal Title: Gastrointestinal Endoscopy Volume: 93 Issue: 6 Pages: 1411-1420.e18
  • DOI: 10.1016/j.gie.2020.10.011
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Direct derivation of human alveolospheres for SARS-CoV-2 infection modeling and drug screening (2021)
  • Author(s): Ebisudani Toshiki、Sugimoto Shinya、Haga Kei、Mitsuishi Akifumi、Takai-Todaka Reiko、Fujii Masayuki、Toshimitsu Kohta、Hamamoto Junko、Sugihara Kai、Hishida Tomoyuki、Asamura Hisao、Fukunaga Koichi、Yasuda Hiroyuki、Katayama Kazuhiko、Sato Toshiro
  • Journal Title: Cell Reports Volume: 35 Issue: 10 Pages: 109218-109218
  • DOI: 10.1016/j.celrep.2021.109218
  • Peer Reviewed / Open Access
• [Journal Article] Wnt Signaling Shapes the Histologic Variation in Diffuse Gastric Cancer (2021)
  • Author(s): Togasaki K, Sugimoto S, Ohta Y, Nanki K, Matano M, Takahashi S, Fujii M, Kanai T, Sato T.
  • Journal Title: Gastroenterology Volume: 160 Issue: 3 Pages: 823-830
  • DOI: 10.1053/j.gastro.2020.10.047
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An organoid-based organ-repurposing approach to treat short bowel syndrome (2021)
  • Author(s): Sugimoto S, Kobayashi E, Fujii M, Ohta Y, Arai K, Matano M, Ishikawa K, Miyamoto K, Toshimitsu K, Takahashi S, Nanki K, Hakamata Y, Kanai T, Sato T.
  • Journal Title: Nature Volume: 592 Pages: 99-104
  • DOI: 10.1038/s41586-021-03247-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mucosal concentrations of N-acetyl-5-aminosalicylic acid is related to endoscopic activity in ulcerative colitis patients with mesalamine. (2020)
  • Author(s): Fukuda T, Naganuma M, Takabayashi K, Hagihara Y, Tanemoto S, Nomura E, Yoshimatsu Y, Sugimoto S, Nanki K, Mizuno S, Mikami Y, Fukuhara K, Sujino T, Mutaguchi M, Inoue N, Ogata H, Iwao Y, Abe T, Kanai T.
  • Journal Title: J Gastroenterol Hepatol. Volume: 35 Issue: 11 Pages: 1878-1885
  • DOI: 10.1111/jgh.15059
  • Peer Reviewed / Open Access
• [Journal Article] Organoid Derivation and Orthotopic Xenotransplantation for Studying Human Intestinal Stem Cell Dynamics (2020)
  • Author(s): Shinya Sugimoto, Masayuki Fujii, Toshiro Sato
  • Journal Title: Methods in Molecular Biology Volume: 2171 Pages: 303-320
  • DOI: 10.1007/978-1-0716-0747-3_21
  • ISBN: 9781071607466, 9781071607473
  • Peer Reviewed / Open Access
• [Journal Article] Linking human intestinal scaffolds and organoids to combat intestinal failure (2020)
  • Author(s): Masayuki Fujii, Shinya Sugimoto, Toshiro Sato
  • Journal Title: Nature Medicine Volume: 26 Issue: 10 Pages: 1517-1518
  • DOI: 10.1038/s41591-020-1096-9
  • Peer Reviewed
• [Journal Article] An Organoid Biobank of Neuroendocrine Neoplasms Enables Genotype-Phenotype Mapping (2020)
  • Author(s): Kawasaki K、Toshimitsu K、Matano M、Fujita M、Kudo Atsushi、Kitagawa Y、Kanai T、Nakagawa H、Sato T, et.al.
  • Journal Title: Cell Volume: 183 Issue: 5 Pages: 1420-1435
  • DOI: 10.1016/j.cell.2020.10.023
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 【消化管症候群(第3版)-その他の消化管疾患を含めて-】空腸、回腸、盲腸、結腸、直腸(上) 炎症性疾患 炎症性腸疾患 総論 (2020)
  • Author(s): 杉本 真也, 金井 隆典
  • Journal Title: 日本臨床 Volume: 消化管症候群III Pages: 113-116
• [Journal Article] -薬剤関連消化管病変の臨床的特徴- 青黛による虚血性腸病変 (2020)
  • Author(s): 杉本 真也, 長沼 誠, 福田 知広, 吉松 裕介, 緒方 晴彦, 岩男 泰, 金井 隆典
  • Journal Title: 胃と腸 Volume: 55 Pages: 908-915
• [Presentation] 小腸移植と再生医療のクロストーク (2023)
  • Author(s): 杉本真也
  • Organizer: 第22回日本再生医療学会総会
  • Invited
• [Presentation] 潰瘍性大腸炎関連腫瘍の自然史の観察に基づくサーベイランス内視鏡戦略 (2023)
  • Author(s): 脇坂悠介, 杉本真也, 高林馨, 高田祐明, 細江直樹, 緒方晴彦, 金井隆典
  • Organizer: 第19回日本消化管学会総会学術集会 (GI Week 2023)
• [Presentation] Dose escalation of oral 5-aminosalicylic acid for ulcerative colitis with Mayo endoscopic subscore of one improves long-term prognosis: a one-year multicentre, open-label, randomized controlled trial (2023)
  • Author(s): Yasuhiro Aoki, Tomohiro Fukuda, Hiroki Kiyohara, Ayumi Yokoyama, Atsushi Nakazawa, Yusuke Yoshimatsu, Shinya Sugimoto, Kosaku Nanki, Yohei Mikami, Kayoko Fukuhara, Shinta Mizuno, et al.
  • Organizer: 18th Congress of European Crohn’s and Colitis Organization (ECCO 2023)
  • Int'l Joint Research
• [Presentation] Intestinal research with replacement of colonic epithelium by organoid transplantation (2022)
  • Author(s): Shinya Sugimoto, Takanori Kanai, Toshiro Sato
  • Organizer: The 108th General Meeting of the Japanese Society of Gastroenterology
  • Int'l Joint Research / Invited
• [Presentation] 液流動による細胞分化誘導と小腸絨毛形成への応用 (2022)
  • Author(s): 杉本 真也, 金井 隆典, 佐藤 俊朗
  • Organizer: 第108回日本消化器病学会総会
• [Presentation] 潰瘍性大腸炎合併腫瘍に対するESD前鑑別診断の検討 (2022)
  • Author(s): 髙林 馨, 岩男 泰, 杉本 真也, 三上 洋平, 細江 直樹, 緒方 晴彦, 金井 隆典
  • Organizer: 第1回Interventional IBD研究会
• [Presentation] Generation of Functional Small Intestine Tissue by Organoid Transplantation (2022)
  • Author(s): Shinya Sugimoto
  • Organizer: The 10th Annual Meeting of Asian Organization for Crohn’s & Colitis
  • Int'l Joint Research / Invited
• [Presentation] Investigation of endoscopic chronological evolution of ulcerative colitis-associated neoplasia (2022)
  • Author(s): Yusuke Wakisaka, Shinya Sugimoto, Yasushi Iwao, Kaoru Takabayashi, Yusuke Yoshimatsu, Hiroki Kiyohara, Takaaki Kawaguchi, Tomohisa Sujino, Naoki Hosoe, Haruhiko Ogata, Yohei Mikami, Takanori Kanai
  • Organizer: The 10th Annual Meeting of the Asian Organization for Crohn's & Colitis
  • Int'l Joint Research
• [Presentation] Evaluation of diagnostic criteria for Sporadic neoplasm in ulcerative colitis (2022)
  • Author(s): Kaoru Takabayashi, Yasuhiro Aoki, Yusuke Yoshimatsu, Hiroki Kiyohara, Shinya Sugimoto, Yohei Mikami, Tomohisa Sujino, Takaaki Kawaguchi, Naoki Hosoe, Yasushi Iwao, Haruhiko Ogata, Takanori Kanai.
  • Organizer: The 10th Annual Meeting of the Asian Organization for Crohn's & Colitis
  • Int'l Joint Research
• [Presentation] Successful Combination Therapy with Infliximab and Cyclosporine A for Refractory Myelodysplastic Syndrome associated Small Intestinal Ulcers: A case report (2022)
  • Author(s): Shogo Sunaga, Hiroki Kiyohara, Kurato Miyazaki, Ryosuke Kasuga, Kaoru Takabayashi, Naoki Hosoe, Takaaki Kawaguchi, Yohei Mikami, Kosaku Nanki, Yusuke Yoshimatsu, Shinya Sugimoto, Tomohisa Sujino, Yasushi Iwao, Haruhiko Ogata, Takanori Kanai
  • Organizer: The 10th Annual Meeting of the Asian Organization for Crohn's & Colitis
  • Int'l Joint Research
• [Presentation] Effectiveness and Durability of Ustekinumab Therapy With or Without Immunomodulators for Ulcerative Colitis Patients (2022)
  • Author(s): Yasuhiro Aoki, Tomohisa Sujino, Takaaki Kawaguchi, Shinya Sugimoto, Fumie Shimada, Yusuke Yoshimatsu, Hiroki Kiyohara, Kosaku Nanki, Yohei Mikami, Kaoru Takabayashi, Naoki Hosoe, Haruhiko Ogata, Yasushi Iwao, Takanori Kanai
  • Organizer: The 10th Annual Meeting of the Asian Organization for Crohn's & Colitis
  • Int'l Joint Research
• [Presentation] Short-term Efficacy of Calcineurin Inhibitors for Intestinal Behcet’s Disease (2022)
  • Author(s): Takaaki Kawaguchi, Masayuki Fukata, Teppei Omori, Hiroki Kiyohara, Shinya Sugimoto, Kosaku Nanki, Tomohisa Sujino, Yohei Mikami, Takanori Kanai
  • Organizer: The 10th Annual Meeting of the Asian Organization for Crohn's & Colitis
  • Int'l Joint Research
• [Presentation] Serum LRG as a novel biomarker of disease and endoscopic activity in patients with Crohn’s disease (2022)
  • Author(s): Yoshiaki Takada, Hiroki Kiyohara, Yohei Mikami, Fumie Shimada, Yuta Matsubara, Ryoya Sakakibara, Yasuhiro Aoki, Masataka Ichikawa, Yusuke Wakisaka, Yuya Hagihara, Shun Tanemoto, Satoko Umeda, Yusuke Yoshimatsu, Keiko Ono, Emi Irie, Shinya Sugimoto, Kosaku Nanki, et al.
  • Organizer: The 10th Annual Meeting of the Asian Organization for Crohn's & Colitis
  • Int'l Joint Research
• [Presentation] Hematologic features in thiopurine treated patients with heterozygote of NUDT15 R139C (2022)
  • Author(s): Ryoya Sakakibara, Hiroki Kiyohara, Yusuke Yoshimatsu, Shinya Sugimoto, Yohei Mikami, Takaaki Kawaguchi, Takanori Kanai
  • Organizer: The 10th Annual Meeting of the Asian Organization for Crohn's & Colitis
  • Int'l Joint Research
• [Presentation] Prognosis in the treatment of the 2nd line anti-TNF inhibitor in patients with ulcerative colitis (2022)
  • Author(s): Hiroki Kiyohara, Takaaki Kawaguchi, Yohei Mikami, Yusuke Yoshimatsu, Shinya Sugimoto, Tomohisa Sujino, Kaoru Takabayashi, Naoki Hosoe, Yasushi Iwao, Haruhiko Ogata, Takanori Kanai
  • Organizer: The 10th Annual Meeting of the Asian Organization for Crohn's & Colitis
  • Int'l Joint Research
• [Presentation] 消化管オルガノイド移植の新潮流 (2022)
  • Author(s): 杉本 真也
  • Organizer: 第43回日本炎症・再生医学会
  • Invited
• [Presentation] UC関連腫瘍診療における肛門直腸移行部観察の意義 (2022)
  • Author(s): 杉本真也, 高林馨, 金井隆典
  • Organizer: 第64回日本消化器病学会大会（JDDW 2022）
• [Presentation] 内視鏡Mayoスコア1の臨床的寛解潰瘍性大腸炎患者に対する経口5-ASA製剤の増量は臨床的再燃を抑制する (2022)
  • Author(s): 福田知広, 青木康浩, 清原裕貴, 横山 歩, 吉松裕介, 杉本真也, 南木康作, 三上洋平, 福原佳代子, 水野慎大, 筋野智久, 牟田口 真, 高林 馨, 諸星雄一, 中澤 敦, 細田泰雄, 緒方晴彦, 岩男 泰, 長沼 誠, 金井隆典
  • Organizer: 第13回日本炎症性腸疾患学会学術集会
• [Presentation] 平坦型潰瘍性大腸炎関連腫瘍の色素散布内視鏡像の特徴 (2022)
  • Author(s): 高林 馨, 岩男 泰, 杉本 真也, 緒方 晴彦, 金井 隆典
  • Organizer: 第115 回日本消化器内視鏡学会関東支部例会
• [Presentation] 自然整復した成人の特発性小腸重積症の一例 (2022)
  • Author(s): 韓可, 吉松裕介, 杉本真也, 清原裕貴, 三上洋平, 筋野智久, 河口貴昭, 高林馨, 細江直樹, 緒方晴彦, 金井隆典
  • Organizer: 日本消化器病学会 関東支部第372回例会
• [Presentation] 潰瘍性大腸炎に対する Texture and Color Enhancement Imaging（TXI）併用内視鏡的活動性評価の有用性 (2022)
  • Author(s): 林 由紀恵, 高林 馨, 吉松 裕介, 清原 裕貴, 杉本 真也, 三上 洋平, 筋野 智久, 細江 直樹, 緒方 晴彦, 金井 隆典
  • Organizer: 第40回日本大腸検査学会総会
• [Presentation] 原因不明の消化管狭窄を来たし、審査腹腔鏡で診断し得た二例 (2022)
  • Author(s): 水島 一郎, 三上 洋平, 河口 貴昭, 岡田 はるか, 須永 将梧, 津軽 開, 戸ヶ崎 和博, 杉本 真也, 清原 裕貴, 南木 康作, 平田 賢郎, 浜本 康夫, 筋野 智久, 高林 馨, 細江 直樹, 緒方 晴彦, 金井 隆典
  • Organizer: 第368回日本消化器病学会関東支部例会
• [Presentation] Proof-of-principle investigation for organoid-based hybrid intestine in a rat short bowel syndrome model (2021)
  • Author(s): Shinya Sugimoto, Eiji Kobayashi, Toshiro Sato
  • Organizer: 17th Congress of the Intestinal Rehabilitation & Transplant Association (CIRTA 2021)
  • Int'l Joint Research
• [Presentation] 小腸上皮オルガノイドによる再生研究 (2021)
  • Author(s): 杉本 真也
  • Organizer: 第42回日本炎症・再生医学会
  • Invited
• [Presentation] Development of a treatment for short bowel syndrome using small intestinal organoids (2021)
  • Author(s): Shinya Sugimoto, Eiji Kobayashi, Masayuki Fujii, Yuki Ohta, Keiko Ishikawa, Kosaku Nanki, Yoji Hakamata, Takanori Kanai, Toshiro Sato
  • Organizer: The 16th Congress of European Crohn’s and Colitis Organisation (ECCO 2021)
  • Int'l Joint Research
• [Presentation] 小腸領域におけるオルガノイドを用いた再生医療開発 (2021)
  • Author(s): 杉本 真也、金井 隆典、佐藤 俊朗
  • Organizer: 第63回 日本消化器病学会大会（JDDW 2021）
• [Presentation] オルガノイドを用いた短腸症候群治療のコンセプト検証 (2021)
  • Author(s): 杉本 真也
  • Organizer: 第12回日本炎症性腸疾患学会学術集会
• [Presentation] 微小変化型ネフローゼ症候群に合併した重症IBD-unclassifiedの一例 (2021)
  • Author(s): 海江田 祐太, 清原 裕貴, 宮崎 蔵人, 三上 洋平, 河口 貴昭, 南木 康作, 筋野 智久, 高林 馨, 杉本 真也, 細江 直樹, 緒方 晴彦, 岩男 泰, 金井 隆典
  • Organizer: 日本消化器病学会関東支部第366回例会
• [Presentation] IBDにおける適切なモニタリング法の選択 クローン病の疾患活動性モニタリング法としての、血清LRG値の有用性の検討 (2021)
  • Author(s): 高田 祐明, 三上 洋平, 青木 康浩, 市川 将隆, 脇坂 悠介, 萩原 裕也, 種本 俊, 梅田 智子, 吉松 裕介, 杉本 真也, 清原 裕貴, 南木 康作, 筋野 智久, 河口 貴昭, 高林 馨, 細江 直樹, 緒方 晴彦, 金井 隆典
  • Organizer: 第39回大腸検査学会総会
• [Presentation] Serum Leucine-rich alpha 2 glycoprotein as a novel surrogate marker of endoscopic activity in patients with Crohn’s disease (2021)
  • Author(s): Ryoya Sakakibara, Hiroki Kiyohara, Hiroaki Takada, Shinya Sugimoto, Kosaku Nanki, Yohei Mikami, Tomohisa Sujino, Kaoru Takabayashi, Takanori Kanai
  • Organizer: The 9th Annual Meeting of the Asian Organization for Crohn's and Colitis (AOCC 2021)
  • Int'l Joint Research
• [Presentation] Incidence and risk factors of venous thromboembolism in inpatients with inflammatory bowel disease (2021)
  • Author(s): Yasuhiro Aoki, Hiroki Kiyohara, Kosaku Nanki, Yohei Mikami, Takaaki Kawaguchi, Tomohisa Sujino, Shinya Sugimoto, Kaoru Takabayashi, Kayoko Fukuhara, Naoki Hosoe, Haruhiko Ogata, Yasushi Iwao, Takanori Kanai
  • Organizer: The 9th Annual Meeting of the Asian Organization for Crohn's and Colitis (AOCC 2021)
  • Int'l Joint Research
• [Presentation] Continuous intravenous cyclosporine therapy improves short term prognosis in patients with severe ulcerative colitis refractory to multiple molecular target therapies (2021)
  • Author(s): Yuta Matsubara, Hiroki Kiyohara, Yasuhiro Aoki, Tomohiro Fukuda, Kosaku Nanki, Yohei Mikami, Takaaki Kawaguchi, Tomohisa Sujino, Shinya Sugimoto, Kaoru Takabayashi, Kayoko Fukuhara, Naoki Hosoe, Haruhiko Ogata, Yasushi Iwao, Makoto Naganuma, Takanori Kanai
  • Organizer: The 9th Annual Meeting of the Asian Organization for Crohn's and Colitis (AOCC 2021)
  • Int'l Joint Research
• [Presentation] UC関連腫瘍に対する内視鏡診断と治療 低分化腺がん・印環細胞がん成分を伴うUC関連腫瘍の検討 (2021)
  • Author(s): 杉本 真也, 岩男 泰, 金井 隆典
  • Organizer: 第102回 日本消化器内視鏡学会総会（JDDW 2021）
• [Presentation] IBD発癌の諸問題・基礎～臨床まで 血管増生を背景とした境界のある発赤は潰瘍性大腸炎関連平坦型dysplasia発見の有用なマーカーである (2021)
  • Author(s): 池端 昭慶, 下田 将之, 岡林 剛史, 茂田 浩平, 清島 亮, 松井 信平, 杉本 真也, 矢作 直久, 金井 隆典, 北川 雄光, 金井 弥栄, 岩男 泰
  • Organizer: 第76回日本大腸肛門病学会学術集会
• [Presentation] COVID -19流行下における炎症性腸疾患の治療満足度に関する調査研究 (2021)
  • Author(s): 原田 沙也加, 河口 貴昭, 清原 裕貴, 杉本 真也, 吉松 裕介, 南木 康作, 三上 洋平, 筋野 智久, 金井 隆典
  • Organizer: 第12回日本炎症性腸疾患学会学術集会
• [Presentation] 炎症性腸疾患患者における静脈血栓塞栓症の臨床的検討 (2021)
  • Author(s): 青木 康浩, 清原 裕貴, 南木 康作, 三上 洋平, 河口 貴昭, 筋野 智久, 杉本 真也, 高林 馨, 福原 佳代子, 細江 直樹, 緒方 晴彦, 岩男 泰, 金井 隆典
  • Organizer: 第12回日本炎症性腸疾患学会学術集会
• [Presentation] 腸管オルガノイドによる上皮置換戦略に基づく再生医療 (2021)
  • Author(s): 杉本真也
  • Organizer: 第20回日本再生医療学会総会
  • Invited
• [Presentation] オルガノイドを用いた短腸症候群治療アプローチの開発 (2021)
  • Author(s): 杉本真也
  • Organizer: 第20回日本再生医療学会総会
  • Invited
• [Presentation] 自己残存小腸から作るハイブリット再生小腸グラフトの開発 (2021)
  • Author(s): 杉本真也
  • Organizer: 第33回日本腸管リハビリテーション・小腸移植研究会
  • Invited
• [Presentation] 短腸症候群に対するオルガノイドによる臓器リモデリング (2020)
  • Author(s): 杉本真也、小林英司、袴田陽二、金井隆典、佐藤俊朗
  • Organizer: 第19回日本再生医療学会総会
  • Invited