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Analysis of Pathophysiology in ICU acquired weakness using proteomics

Research Project

Project/Area Number 20H03780
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 55060:Emergency medicine-related
Research InstitutionOsaka University

Principal Investigator

FUJINO Yuji  大阪大学, 大学院医学系研究科, 教授 (50252672)

Co-Investigator(Kenkyū-buntansha) 今井 由美子  国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 ワクチン・アジュバント研究センター, プロジェクトリーダー (50231163)
吉田 健史  大阪大学, 大学院医学系研究科, 特任講師(常勤) (50725520)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2020: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords急性呼吸不全 / 人工呼吸 / ICU acquired weakness / 筋力低下 / プロテオミクス解析
Outline of Research at the Start

ICU ACQUIRED WEAKNESS (ICU-AW)の原因として呼吸筋の廃用性萎縮、呼吸筋疲労、炎症が考えられている。本研究計画ではICU-AWの病態を解明するために動物を用いて廃用性筋萎縮モデルと呼吸筋疲労(炎症を伴うものと伴わないものの2種類)モデルにおいて横隔膜筋細胞におけるRNA解析と血液におけるプロテオミクス解析を網羅的に行い、ICU-AWにおいて特異的に発現する蛋白質を探索するとともに原因別の発現パターンの違いの評価を行う。動物モデルでの検討と並行してICU-AW患者の血液サンプルを集積し、動物での検討後に解析を行う。患者データを動物モデルでの結果と比較し病態を考察する。

Outline of Final Research Achievements

At the beginning we had a plan to evaluate ICU-AW patients due to usual ARDS. However, most of the patients we recruited are ADRS due to COVID-19. COVID-19 patients usually show stronger respiratory distress than the usual ARDS patients. Therefore, we switched to a new animal model to evaluate the effects of asynchrony between patients' spontaneous breaths and mechanical supports on the development of lung injury. We could have shown asynchrony can promote lung injury and the type of asynchrony affects injury differently. In addition, we could show there are 2 types in COVID-19 patients in terms of ventilatory distribution, which require different strategies. Now we are evaluating the relationship with ICU-AW using blood samples from patients.

Academic Significance and Societal Importance of the Research Achievements

ICU acquired weakness (ICU-AW)の発生原因は多岐に渡るが、人工呼吸器と患者自発呼吸の不同調も原因であることは知られていなかった。近年のCOVID-19蔓延のように多数の呼吸不全患者が発生している状況では筋弛緩を含む適切な判断の有無により死亡者数の大きな違いに繋がる可能性がある。さらにICU-AWのような長期的影響を検討することは国民衛生に大きな意味を持つ。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (11 results)

All 2023 2022 2021

All Journal Article (11 results) (of which Open Access: 7 results,  Peer Reviewed: 6 results)

  • [Journal Article] Association between tracheostomy and survival in patients with coronavirus disease 2019 who require prolonged mechanical ventilation for more than 14 days: A multicenter cohort study2023

    • Author(s)
      Tanaka Aiko、Uchiyama Akinori、Kitamura Tetsuhisa、Sakaguchi Ryota、Komukai Sho、Enokidani Yusuke、Koyama Yukiko、Yoshida Takeshi、Iguchi Naoya、Sobue Tomotaka、Fujino Yuji
    • Journal Title

      Auris Nasus Larynx

      Volume: 50 Issue: 2 Pages: 276-284

    • DOI

      10.1016/j.anl.2022.06.002

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Positive end-expiratory pressure and prone position alter the capacity of force generation from diaphragm in acute respiratory distress syndrome: an animal experiment2022

    • Author(s)
      Firstiogusran Andi Muhammad Fadlillah、Yoshida Takeshi、Hashimoto Haruka、Iwata Hirofumi、Fujino Yuji
    • Journal Title

      BMC Anesthesiology

      Volume: 22 Issue: 1 Pages: 373-373

    • DOI

      10.1186/s12871-022-01921-0

    • Related Report
      2022 Annual Research Report
    • Open Access
  • [Journal Article] Association between early tracheostomy and patient outcomes in critically ill patients on mechanical ventilation: a multicenter cohort study2022

    • Author(s)
      Tanaka Aiko、Uchiyama Akinori、Kitamura Tetsuhisa、Sakaguchi Ryota、Komukai Sho、Matsuyama Tasuku、Yoshida Takeshi、Tokuhira Natsuko、Iguchi Naoya、Fujino Yuji
    • Journal Title

      Journal of Intensive Care

      Volume: 10 Issue: 1 Pages: 19-19

    • DOI

      10.1186/s40560-022-00610-x

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prone Position Minimizes the Exacerbation of Effort-dependent Lung Injury: Exploring the Mechanism in Pigs and Evaluating Injury in Rabbits2022

    • Author(s)
      Yoshida Takeshi、Engelberts Doreen、Chen Han、Li Xuehan、Katira Bhushan H.、Otulakowski Gail、Fujino Yuji
    • Journal Title

      Anesthesiology

      Volume: 136 Issue: 5 Pages: 779-791

    • DOI

      10.1097/aln.0000000000004165

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Individualized ventilatory management in patients with COVID-19-associated acute respiratory distress syndrome2021

    • Author(s)
      Taenaka Hiroki、Yoshida Takeshi、Hashimoto Haruka、Iwata Hirofumi、Koyama Yukiko、Uchiyama Akinori、Fujino Yuji
    • Journal Title

      Respiratory Medicine Case Reports

      Volume: 33 Pages: 101433-101433

    • DOI

      10.1016/j.rmcr.2021.101433

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prone Position Reduces Spontaneous Inspiratory Effort in Patients with Acute Respiratory Distress Syndrome: A Bi-Center Study2021

    • Author(s)
      Yoshida Takeshi、Tanaka Aiko、Roldan Rollin、Quispe Roc?o、Taenaka Hiroki、Uchiyama Akinori、Fujino Yuji
    • Journal Title

      American Journal of Respiratory and Critical Care Medicine

      Volume: - Issue: 11 Pages: 1437-1440

    • DOI

      10.1164/rccm.202012-4509le

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Monitoring the patient for a safe-assisted ventilation2021

    • Author(s)
      Yoshida Takeshi、Fujino Yuji
    • Journal Title

      Current Opinion in Critical Care

      Volume: 27 Issue: 1 Pages: 1-5

    • DOI

      10.1097/mcc.0000000000000788

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 付随する問題 人工呼吸器関連肺傷害(VALI)2021

    • Author(s)
      吉田 健史
    • Journal Title

      救急・集中治療

      Volume: 33 Pages: 1297-1303

    • Related Report
      2021 Annual Research Report
  • [Journal Article] ARDS患者における肺保護換気2021

    • Author(s)
      妙中 浩紀、吉田 健史
    • Journal Title

      人工呼吸

      Volume: 38 Pages: 106-111

    • Related Report
      2021 Annual Research Report
  • [Journal Article] ARDSにおける自発呼吸を考える2021

    • Author(s)
      橋本 明佳、吉田 健史
    • Journal Title

      人工呼吸

      Volume: 38 Pages: 131-136

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 自発呼吸の是非2021

    • Author(s)
      吉田 健史
    • Journal Title

      ICUとCCU

      Volume: 45 Pages: 173-179

    • Related Report
      2021 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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