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Deciphering functional significance of newly generated neurons at postnatal stage investigated by multi-photon 4D imaging

Research Project

Project/Area Number 20H04122
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 59040:Nutrition science and health science-related
Research InstitutionKyoto University

Principal Investigator

Sakamoto Masayuki  京都大学, 生命科学研究科, 特定准教授 (00777865)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2021: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2020: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords嗅覚 / 2光子イメージング / カルシウムセンサー / 顆粒細胞 / カルシウムイメージング / 4Dイメージング / 嗅球 / 高次脳機能 / 生後発達期
Outline of Research at the Start

ヒトを含めた哺乳類の生後発達期の脳においても神経幹細胞が存在し、ニューロン新生が起こっている。ところが、これら新生ニューロンが嗅覚依存的な学習過程において、どのように機能しているかについてはよく分かっていない。本研究では、神経活動を計測するイメージング技術を用いて、新生ニューロンの機能について、回路レベルで明らかにする。本研究により、生後発達期におけるニューロン新生の理解を飛躍的に深化させ、健康な脳を維持するための予防法・治療法の技術基盤の確立を目指す。

Outline of Final Research Achievements

Neurogenesis occurs continuously in the mammalian postnatal brain, including humans, and newborn neurons are incorporated into the neural circuit in the olfactory bulb as granule cells. However, it is not well understood how these neurons respond to odor stimuli. In this study, we analyzed the function of granule cells using calcium imaging. First, we established an in vivo 4D imaging technique to monitor activity from different layers of the olfactory bulb. Then, we performed in vivo calcium imaging of granule cells and found that their activity was attenuated by the repeated presentation of the same odor stimulus. On the other hand, the activity of granule cells increased as learning progressed during the go/no-go olfactory discrimination task.

Academic Significance and Societal Importance of the Research Achievements

自閉症をはじめとする発達障害のメカニズムの解明および治療法の確立は最重要課題のひとつとなっているが、これら多くの病態について、生後発達期~若年成人期におけるニューロン新生低下による高次脳機能障害が示唆されている。したがって、生後発達期に産まれたニューロンの機能について、単一ニューロンレベルあるいはネットワークレベルで理解することは、ニューロン新生の基礎的理解にとどまらず、発達障害などの脳機能障害の治療法の確立し、健康・医療の質の向上ために重要であると考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (17 results)

All 2023 2022 2021 2020

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (12 results) (of which Int'l Joint Research: 3 results,  Invited: 11 results)

  • [Journal Article] A multicolor suite for deciphering population coding in calcium and cAMP<i>in vivo</i>2023

    • Author(s)
      Yokoyama Tatsushi、Manita Satoshi、Uwamori Hiroyuki、Tajiri Mio、Imayoshi Itaru、Yagishita Sho、Murayama Masanori、Kitamura Kazuo、Sakamoto Masayuki
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2023.01.06.522686

    • Related Report
      2022 Annual Research Report
    • Open Access
  • [Journal Article] In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice.2022

    • Author(s)
      Sakamoto M, Ota K, Kondo Y, Okamura M, Fujii H, Bito H.
    • Journal Title

      Star Protocols

      Volume: 3 Issue: 2 Pages: 101421-101421

    • DOI

      10.1016/j.xpro.2022.101421

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo2022

    • Author(s)
      Sakamoto Masayuki, Inoue Masatoshi, Takeuchi Atsuya, Kobari Shigetaka, Yokoyama Tatsushi, Horigane Shin-ichiro, Takemoto-Kimura Sayaka, Abe Manabu, Sakimura Kenji, Kano Masanobu, Kitamura Kazuo, Fujii Hajime, Bito Haruhiko
    • Journal Title

      Cell Reports Methods

      Volume: 2 Issue: 2 Pages: 100168-100168

    • DOI

      10.1016/j.crmeth.2022.100168

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Imaging Voltage with Microbial Rhodopsins2021

    • Author(s)
      Zhang Xiao Min、Yokoyama Tatsushi、Sakamoto Masayuki
    • Journal Title

      Frontiers in Molecular Biosciences

      Volume: 8 Pages: 733829-733829

    • DOI

      10.3389/fmolb.2021.738829

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparative Studies of the Fluorescence Properties of Microbial Rhodopsins: Spontaneous Emission Versus Photointermediate Fluorescence2020

    • Author(s)
      Kojima Keiichi、Kurihara Rika、Sakamoto Masayuki、Takanashi Tsukasa、Kuramochi Hikaru、Zhang Xiao Min、Bito Haruhiko、Tahara Tahei、Sudo Yuki
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 124 Issue: 34 Pages: 7361-7367

    • DOI

      10.1021/acs.jpcb.0c06560

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] Imaging voltage and biochemical signaling in vivo2022

    • Author(s)
      Masayuki Sakamoto
    • Organizer
      Merocyanine 540/Flash meeting
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fluorescent biosensors for probing neuronal activity and biochemical signaling2022

    • Author(s)
      Masayuki Sakamoto
    • Organizer
      Cell Biology, Developmental Biology, and Systems Biology Course Meeting
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 神経活動可視化のための蛍光プローブの開発と応用2022

    • Author(s)
      坂本雅行
    • Organizer
      東京理科大学 第11回パラレル脳研究部門勉強会・セミナー
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Fluorescent biosensors for probing neuronal activity and biochemical signaling2022

    • Author(s)
      Masayuki Sakamoto
    • Organizer
      Hamburg University - Kyoto University Joint Symposium “Cellular mechanisms of learning and memory”
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 神経活動可視化のための蛍光プローブの開発と応用2022

    • Author(s)
      坂本雅行
    • Organizer
      第五回これからの神経回路研究の会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Engineering microbial rhodopsins for genetically encoded voltage indicators2021

    • Author(s)
      Masayuki Sakamoto
    • Organizer
      第44回日本神経科学大会JNS-CAN合同シンポジウム「Understanding of synaptic and neural circuits through advanced optical technologies.」
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 膜電位感受性プローブを用いた神経活動計測2021

    • Author(s)
      坂本雅行
    • Organizer
      第64 回日本神経化学会大会シンポジウム「先端的イメージング技術で読み解く高次脳機能の仕組み」
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 膜電位感受性プローブを用いた神経活動計測2021

    • Author(s)
      坂本雅行
    • Organizer
      第42 回生体膜と薬物の相互作用シンポジウム
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Characterizing and engineering microbial rhodopsins for genetically encoded voltage indicator2020

    • Author(s)
      Masayuki Sakamoto, Xiao Min Zhang, Rika Kurihara, Keiichi Kojima, Yuki Sudo, Haruhiko Bito
    • Organizer
      遺伝研研究会「哺乳類脳の機能的神経回路の構築メカニズム」
    • Related Report
      2020 Annual Research Report
  • [Presentation] 膜電位感受性プローブの開発とその応用2020

    • Author(s)
      坂本雅行
    • Organizer
      鹿児島大学医歯学総合研究科セミナー
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Imaging voltage in neurons with genetically encoded indicators.2020

    • Author(s)
      Masayuki Sakamoto
    • Organizer
      生理研記憶研究会 “Synapse and System Plasticity of Learning and Memory”
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genetically encoded voltage indicators and their application.2020

    • Author(s)
      Masayuki Sakamoto
    • Organizer
      遺伝研研究会「哺乳類脳の機能的神経回路の構築メカニズム」
    • Related Report
      2020 Annual Research Report
    • Invited

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Published: 2020-04-28   Modified: 2024-01-30  

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